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Genetically modified Test 3
on GM foods, cancer, and cloning!
26
Biology
Undergraduate 3
11/05/2008

Additional Biology Flashcards

 


 

Cards

Term
Why Cell division?
Definition

Growth of an organism

replacement of aging cells

reproduction

Term
Recumbent DNA basic steps
Definition

Basic Steps

1. isolate DNA from animal plant

2. cut out gene using a restriction enzyme that leaves sticky ends (areas of nuclotides that are no longer paired)

3. Make a complimentary cut in the plasmid

4. implant gene into plasmid using a ligase (ties nucleotides together)

5. insert the plasmid into the bacteria

6. grow the bacteria in large numbers

7. Harvest the gene products

 

1st case - insulin production for diabetics 

Term
Old and new ways of getting insulin
Definition

Old- from the pancreas of pigs and cattle

new- by genetically engineering them in E. Coli vats (much cheaper)

Term

Stem Cell research

What is it

 

Definition

Cells have the ability of self-renewal (make copies of itself) these stem cells have no other function that to make other cell types that can differenciate into other cell types

undifferentited means that that cell can only have specific range of duties

 

Term

Two types of Stem Cell research

what is the difference

Definition

Adult- multipontent, difficult to find, fully formed, not very controversial, hard to isolate and culture, may have more potential than previously thought

 

Embryonic -  Pluripotent, get them from very early stage of baby (only a few days old), easy to grow, they divide infinitely, but very controversial

Term
Therapeutic cloning?
Definition

Creating your own embryo

cells from foreign source have possible rejection in your body so you make these to match your own embyronic stem cells

you ahve diploid cells with only your genes

you harvest the cells from the blastocysts and you know that they are going to be undifferentiated because they are your own cells

 

opposed people say that it could lead to your own reproductive clone

Term
Technology of fingerprinting
Definition

fingerprinting is used in murder cases

99.9% of our blood is the same but there is .1% that is different in every person

we use it tocompare different genes

junk DNA does not code for anything

Term
SHort tandem repeats
Definition
are repititions of DNA sequences that are different in every person. they vary in size and sequence. It is found in their junk DNA
Term
PCR
Definition
Polymerase chain reaction is used to make several copies of DNA - it is a primer
Term
GM Foods
Definition
Corn and soybeans are found in almost everything! almost half of soybeans are GM and almost a third of corn is GM
Term
Electrophoresis
Definition
a tool used to study many different fragments of DNA. DNA fragments are separated on the bases of size using gel electrophoresis (moving by electricity) a gel with the consistency of jello has small pores, you add your sample to the depressions (wells) and charge it, DNA is negatively charged so you a apply a positive charge to make them move, the fragments that are short reach the bottom and the longer ones spread out throughout the gel
Term
Benefits of GM Foods
Definition
Have their own pesticides and have resistance to herbicides, have more nutrients, grow faster and better, cheaper oral vaccines, resistance to diseases, biodegradable plastics
Term
Downfalls of GM foods
Definition
crops with pesticides resistance can kill wildlife species, super pests, herbicides resistance producing super weeds, increase herbicide use, requiring farmers to buy only a specific type of herbicide
Term
Risks of GM foods
Definition
Food safety because by genetically modifying the organisms we are going to create new allergens
Term
Cancer
Definition
disease of run away cel division
signel cell with damage tht no longer recongnize the proceess that controls its division
Term
Characteristics of Cancer Cells
Definition
Divide without external signals
dont ever stop dividing- they have telomeres but they also have telomerace that rebuilds the telomeres when the cells divide instead of shortening
dont ever become sepcialized
dont respond to check points in the cell cycle
Term
Three stages of Cancers
Definition
Initiations- DNA become damaged, repair can occur, apoptosis, but can lead to irreversble DNA Damage to critical genes Promotin-for it to become a tumor both the promotion and promotion stages occur, larger number of initiated cells the higher risk for cancer, cell is stimulated to divide, promoter agent maybe the same agent or different from the initiation agent, gradual

Progression - cells irreversibly become cancer invade and kill other tissue

Term
Carcinomas
Sarcomas
Leukemia
Definition
Epithelial cells, 80percent of all cancers

supportive or connective tissue

blood cancers

Term
Chances of American man getting cancer?

american woman?

Definition
men 1 in 2

women is 1 in 3

Term
Three most prevalent types of cancer in men
Definition
prostate lung colon
Term
What are the prevalent types of cancer among women
Definition
breast lung colon
Term
Types of treatment
Definition
Radiation, kimotherapy, HER2 therapy (molecules manufactured to bind with HER2)
Term
P53 gene
Definition
viruses can shut it down, genetically modified viruses remove the anti-P53 genes
Term
Cancer cells produce what hormone?
Definition
VEGF which they use to trick the brain into formulating blood vessels in their area (there are new drugs to bind receptors and block VEGF)
Term
transcription
Definition
DNA unzips, just the single gene is transcribed - small area of the DNA is unzipped, the nucleotides pair with the exposed bases on one strand (the RNA polymerase) creating the messenger RNA molecule, Then the trasndcript breaks free of the DNA and the DNA zips back up it is then processed to make a functional messenger RNA
Term
Translation
Definition
Messenger RNA leaves the nucleus and attaches itself to a ribosome, the MRNA is carrying the genetic codes, transfer RNAs transfer the free amino acids and bring them to the ribosome, anticodon regions of these bucleotides are compliments of the condons for MRNA, the peptide bonds form bringing along their amino acids which then form bonds between them creating a of amino acids, then the ribosome moves to the next condon and so forth until the code on the MRNA is translated into the messenger protein
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