Term
| When weas the CRISPR/Cas system first used? |
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Definition
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Term
| How is Cas9 directed to sequences? |
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Definition
| by gRNA, which is formed of crRNA-the CRISPR array transcripts, and tracrRNA - which recognizes the repeats |
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Term
| How does the CRISPR system protect against phages in nature? |
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Definition
| When the phage enters the cell, certain cas proteins record ‘protospacers’ in its DNA. These are encoded in DNA near the CRISPR array, with direct repeats and spacers that correlate to those of the phage. The transcripts of this array are called crRNAs, and hybridize with a tracrRNA that recognizes the repeats to form a guideRNA, or gRNA, which targets the cas9 endonuclease, which cuts the virus DNA into pieces, leaving double stranded (DS) breaks. |
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Term
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Definition
| Any sequence flanked by a protospacer adjacent motif (PAM) |
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Term
| How does the DSBs caused by cas9 cause when a) there is donor DNA and b) when there isnt? |
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Definition
Homology directed repair knocks in DNA
NHEJ leads to mutations |
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Term
| What is the big benefit to CRISPR-cas9? |
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Definition
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