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Gene Expression
DNA and RNA
30
Biology
Undergraduate 1
11/29/2007

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Term
Gene
Definition
section/segments of DNA
Term
Expression
Definition
make proteins, transciption and translation;
Term
Central Dogma Theory
Definition
general way of thinking about flow of genetic info.
Term
RNA
Definition
nucleic acid composed of nucleotides
Term
RNA nucleotides
Definition

Have three parts:

 

sugar (ribose)

phosphate

nitrogen base

 

The nitrogen base has two parts:

purines: adenine and guanine

pyrimidines: cytosine and uracil (only in RNA)

Term
polypeptide chains
Definition

sequences of amino acids that correspond to genes;

 

a proteins primary structure;

Term
mRNA
Definition

the only class of RNA that carries the protein-building codes.

 

transcribed genes that specify proteins;

Term
Ribosomal RNA and transfer RNA
Definition
Term
RNA polymerse
Definition
adds ribonucleotides one at a time to the end of a growing strand of RNA.
Term
promoter
Definition
Start signal, a base sequence in DNA to which RNA polymerases bind and prepare for transcription.
Term
Transcription
Definition

the first step in protein synthesis;

 

a sequence of ncleotide bases is exposed in an unwound region of a DNA strand;

 

 

Term
exons
Definition

protein coding base sequences that are interrupted by nncoding sequences (introns);

 

(all introns are snipped off before the transcript reaches the cytoplasm)

Term
alternative splicing
Definition
Term
codons
Definition

bse triplets in mRNA strands;

 

Term
genetic code
Definition
te set of sixty-four different codons;
Term
anticodon
Definition
a ribonucleotide base triplet that can base-pair with a complementary codon in an mRNA transcript, found in tRNA strands.
Term
wobble effect
Definition

happens when tRNA and mRNA strands bind together;

 

the freedom in codon-anticodon pairing at a base;

 

 

 

Term
describe the interactions of tRNA and mRNA
Definition

intereactions between tRNA and mRNA take place at ribosomes. 

 

Only mRNA carries DNA's protein-building instructions from the nucleus into the cytoplasm;

 

tRNA delivers amino acids to ribosomes. Their anticodons base-pair with codons in the order specified by mRNA.

Term
What are the three stages of translation?
Definition
initiation, elongation, and termination
Term
elongation
Definition

a polypeptide chain is synthesized while the mRNA passes between the two ribosomal subunits;

 

i.e. athread being moved through the eye of a

 needle;

 

RNA polymerse adds free complementary nucleotides to DNA strand;

Term
initiation
Definition

RNA polymerse (tRNA) binds to DNA at promoter region;

 

DNA polymerse unravel DNA dbl helix;

Term
Termination
Definition

RNA polymer reaches stop sequence and stops transcription;

 

 

Term
Translation
Definition

process of RNA makind protein;  occurs in the cytoplasm

 

methionine is the 1st amino acid in protein process in humans (20 total);

Term

Mutation

Definition
change in DNA sequence
Term
base-pair substitution
Definition
mismatched nitrogen base pair;
Term
frameshift mutations
Definition

fall into to categories:

 

insertion mutation - addition of nitrogen base

 

deletion mutation- removal of a nitrogen base

Term
transposons
Definition

jump around in the genome;

 

segements of DNA (or sopies) that move spontaneously to a new location in a chromosome or even to a different chromosome.

Term
ionizing radiation
Definition

form of radiation with enough energy to eject electrons from atoms;

 

may cause mutations in DNA if exposed in large doses;

Term
nonionizing radiation
Definition
excites electrons to a higher energy level;
Term
What are the most common types of gene mutations?
Definition
base-pair substitutions, insertions and deletions;
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