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Details

FST 104 L Exam 1
Lab Exam
11
Microbiology
Undergraduate 1
04/25/2015

Additional Microbiology Flashcards

 


 

Cards

Term
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Definition

SS agar Test

Contains Brilliant Green and Bile Salt - Slective against G+

 

Lactose Fermenters - Magenta

Non Fermenters - Pale

 

http://rci.rutgers.edu/~microlab/applied%20micro/schedulelinks/salmonellashigella.htm

Term
[image]
Definition

Triper Sugar Iron Agar

 

Glucose Fermentors - Red Slant, Yellow Butt

Lactose and/or Sucrose - All Yellow

H2S Production - Black

Term
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Definition

Lysine Decarboxylase Broth

 

Use: Diffentiation of Salmonella

Positive Result: Purple (lyssine Decaboxylase Present)

Negitive Resut: Yellow 

 

Produces: Cadaverine

Term
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Definition

Brilliant Green Lactose Bile Test

Use: Colifrorm Detection

Selects: G+ bacteria that ferment lactose

Enezyme: Formate Haydrogenlyase -> Gas Present

 

Term
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Definition

MacConkey Agar

Use: Isolation of Enterobacteriaceae

Positive: Magenta Color - Lactose fermentor - E Coli

Yelow: Enterics which do not ferment lactose

Term
[image]
Definition

Phenol Red Broth + Dulcitol (PRD)

Use: Differentiaion of Salmonella

Fermentation of Dulcitol -> Yellow Color

Gas Formation + Yellow = Salmonella

Term
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Definition

Bismuth Sulfite Agar

  • Isolalation of Salmonella
  • Enzyme: Sulfite Reductase
  • Brilliant Green - Slelective against G+
  • Salmonela Produces H2S from sulfite, creates elemental bismuth to make a metalic sheen. 
  • Positive Reation: Brown and Sheen
Term
[image]
Definition

Eosin Methylene Blue Agar

  • Coliform Detection
  • Dark Colonies/Metalic -> Lactose Fermenters
  • Pale/Light Colonies -> Non Lactose Fermenters
  • Gram + -> Usualy do not Grow
Term
[image]
Definition

Simmons Citrate Agar

  • Organisms who only consume citrate as a carbon source can grow
  • Positive Result is Blue
  • Salmonella can consume citrate, E Coli Cannot
Term
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Definition

Mannitol Yolk Prlymyxin Agar - MYP

  • Inhibits growth of G-
  • Use: Selection of Bacillus Species
  • Enzyme: Lecithinase ->Cloudiness around colony
  • Manitol Fermentation - Bacillus -> Yellow Colonies
  • Selection and Diffentiation
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