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From Gene to Protein
Campbell Chapter 17: From Gene to Protein
40
Biology
10th Grade
02/16/2011

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Term
auxotroph
Definition
mutant strains unable to synthesize certain essential molecules from minimal ingredients: nutritional mutants
Term
one gene-one polypeptide
Definition
Beadle and Tatum's hypothesis that each gene coded for one polypeptide NOT specifically an enzyme or a protein
Term
transcription
Definition
making a mRNA copy of DNA
Term
mRNA
Definition
messenger RNA; single strand complementary copy of the DNA transcript
Term
translation
Definition
synthesis of a polypeptide on a ribosome
Term
RNA processing
Definition
modification of the mRNA before it leaves the nucleus; intron excision, poly A cap, and 5' cap
Term
primary transcript
Definition
initial (unprocessed) RNA transcript of DNA
Term
triplet code
Definition
3 nucleotide sequence of DNA
Term
template strand
Definition
DNA strand of the double helix that is transcribed into mRNA
Term
codon
Definition
mRNA base triplets
Term
reading frame
Definition
starting to read the mRNA genetic code from the correct starting point so the correct sequence of nucleotides can be turned into the correct amino acids
Term
RNA polymerase
Definition
enzyme the pries the two stranded DNA apart and adds RNA nucleotides to form the mRNA
Term
transcription unit
Definition
stretch of DNA transcribed into an RNA molecule
Term
promoter
Definition
region of DNA were RNA polymerase attaches and initiates transcription
Term
transcription factor
Definition
collection of proteins in eukaryotes that mediates the binding of RNA polymerase and the initiation of transcription
Term
TATA box
Definition
promoter DNA sequence in the initiation complex of transcription
Term
terminator
Definition
RNA base sequence that functions as the termination signal to transcription
Term
5' cap
Definition
modified guanine nucleotide that is attached to the end of a mRNA molecule-protects the mRNA from degradation and helps it attach to a ribosome in the cytoplasm
Term
poly A tail
Definition
30-200 adenine molecules added to the end of a mRNA to prevent degradation in the cytoplasm and help it attach to the ribosome
Term
RNA splicing
Definition
the removal of introns from a mRNA molecule
Term
introns
Definition
non-coding DNA sections; junk DNA
Term
exons
Definition
expressed DNA; codes for a polypeptide
Term
spliceosome
Definition
proteins that remove introns from the mRNA
Term
tRNA
Definition
transfer RNA; carries the amino acids to the ribosome during polypeptide synthesis
Term
anticodon
Definition
nucleotide triplet on the tRNA that is complementary to the codon on the mRNA
Term
wobble
Definition
relaxation of base pairing in the third nucleotide of a codon
Term
aminoaceyl-tRNA synthetase
Definition
enzyme that assists in the joining of amino acids to tRNA molecules
Term
rRNA
Definition
ribosomal RNA; makes up the ribosmes; two subunits, the large and small
Term
A site
Definition
holds the tRNA molecule carrying the next amino acid to be added to the polypeptide chain
Term
P site
Definition
holds the tRNA carrying the growing polypeptide
Term
E site
Definition
discharges tRNA molecules from the ribosome
Term
polyribosomes
Definition
strings of ribosomes
Term
signal peptide
Definition
protein that targets each polypeptide to a specific location
Term
mutations
Definition
changes in the genetic material of a cell
Term
base pair substitution
Definition
replacement of one nucleotide and its partner in the complementary strand with another pair of nucleotides
Term
missense mutations
Definition
altered codons still code for an amino acid and makes sense....just not the correct sense
Term
nonsense mutations
Definition
alterations that change an amino acid codon to a stop signal
Term
frameshift mutations
Definition
occurs when the number of nucleotides inserted or deleted is not a multiple of 3
Term
mutagens
Definition
physical and chemical agents that interact with DNA to cause mutations
Term
Ames test
Definition
test for the mutagenic activity of a chemical
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