Term
| Correctly follow the steps for an acid elution |
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Definition
1. Wash DAT + cells 1x with saline in a 12x75 tube, then wash 4x with 1x working wash solution, if antibody is present wash 8x with 1x working wash solution.
2. Remove final supernatant with pipet and save in labeled 12x75 tube
3. Place 20 drops of washed cells in labeled 12x75 tube
4. Add 20 drops of eluting solution and resuspend
5. Centrifuge at 3400rpm for 60 seconds
6. Transfer supernatant to clean tube
7. Add buffering solution to restore pH
8. Centrifuge to remove debris, transfer to clean tube
9. Perform panel on eluate at 37°C and AHG, use 2 drops from final wash as a control |
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Term
| Discuss the significance of the results obtained |
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Definition
| used to demonstrate HDN, demonstrate positive DAT, produce a single antibody solution, and demonstrate antigen by absorbing antibody |
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Term
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Definition
| cells must be less than 72 hours old, eluate must be returned to normal pH, positive DAT due to complement will not yield a positive eluate, eluates are unstable and should be tested immediately, and amount of eluate depends on amount of antibody bound to red cell. |
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Term
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Definition
| An elution is the removal of antibody that is absorbed onto red cells surfaces either in vivo or in vitro. |
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Term
| What are four possible methods for elutions? |
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Definition
| The four methods for elutions are (1) heat elution at 56°C (2) heat elution at 42-44°C (3) Ether elution and (4) acid elution. |
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