Term
|
Definition
| consists of a simple biconvex lens |
|
|
Term
|
Definition
-consists of 2 lens systems
-the most widely used |
|
|
Term
|
Definition
-magnifies the specimen
-produces a real image within the body tube |
|
|
Term
|
Definition
| magnifies the real image in the body tube to produce a virtual image that is seen by the eye while viewing the specimen |
|
|
Term
|
Definition
| calculated by multiplying the (initial magnification of the objective being employed) by (magnification of ocular) |
|
|
Term
|
Definition
| -ability of the lens to show 2 closely adjacent points as both distinct and separate |
|
|
Term
|
Definition
| organic compound which contains a benzene ring plus a chromophore and auxochrome group |
|
|
Term
|
Definition
| anionic in that the chromogen exhibits a negative charge |
|
|
Term
|
Definition
| cationic in that the chromogen exhibits a positive charge |
|
|
Term
| simple staining (direct stain) |
|
Definition
-for visualization of morphological shape (cocci, spiral) and arrangements (chains, clusters)
-to make direct microscopic cell count
-use crystal violet, safranin, carbol fuchsin
-(-) charged cell-wall combine w/ (+) charged methlylene blue ion |
|
|
Term
|
Definition
-separation into groups: gram stain, acid-fast stain
-visualization of structures: flagella, capsule, spore, nuclear stain |
|
|
Term
|
Definition
1. provides contrast b/w microbe and its surrounding background that shows morphological types
2. provides contrast which permits study of internal structures (cellwall, vacuoles) |
|
|
Term
|
Definition
-a differential stain
-divide org into gram (+) and gram (-) |
|
|
Term
|
Definition
| -retain basic dye following decolorization |
|
|
Term
|
Definition
| -cell walls contain more lipids which are soluble in alcohol and acetone |
|
|
Term
| 4 diff solutions in gram stain |
|
Definition
-basic dye, mordant, decolorizing agent, counterstain
-basic dye is crystal violet |
|
|
