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Exam 5 Superficial & Cutaneous Mycoses
Fungi
66
Biology
Undergraduate 3
04/12/2015

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Term
Name 4 Superficial mycoses MOs
Definition
Malassezia furfur
Hortaea werneckii
Piedraia hortae
Trichosporon beigelii
Term
Which Superficial mycoses MO has KOH prep = "Spaghetti & Meatballs"
Definition
Malassezia furfur
Term
Malassezia furfur is attributed to what type of Ringworm?
Definition
Tinea versicolor / Pityriasis versicolor
Term
What does Malassezia furfur require to grow?
Definition
Fatty acids
Term
What can you supplement your Sabs/other media with to get Malassezia furfur to grow?
Definition
Sterile olive oil
Term
What are the 2 special media used for Malassezia furfur?
Definition
GYP-S
Dixon's agar
Term
Tinea niger is caused by which MO?
Definition
Hortaea werneckii
Term
Black Peidra is caused by which MO?
Definition
Piedraia hortae
Term
White Piedra is caused by which MO?
Definition
Trichosporon beigelii
Term
Which superficial mycoses produces a hard dark brown nodule on shaft?
Definition
Black Piedra
Term
Which superficial mycoses produces a nodule that is easy to crush and appears yellow or pale brown?
Definition
White Piedra
Term
What is Tinea niger/Hortaea werneckii in nature?
Definition
Saprophyte
Term
How would you diagnose Hortaea werneckii?
Definition
KOH
Term
Can you diagnose Piedraia hortae microscopically?
Definition
Yes
Term
Which superficial mycoses infects axillary, facial, genital, and scalp hair?
Definition
Trichosporon beigelii / White Piedra
Term
What are the 3 genera of Dermatophytes causing infections?
Definition
Microsporum
Epidermophyton
Trichophyton
Term
True or False:
Dermatophytes are involved in the destruction of the skin, hair, and nails
Definition
True
Term
Define the following:
Anthropophilic
Definition
Prefers to grow on humans rather than other animals of in the soil
Term
Define the following:
Zoophilic
Definition
Preferential host is an animal other than humans
Term
Where would you find the following:
Tinea capitis
Definition
Head
Term
Where would you find the following:
Tinea corporis
Definition
Body
Term
Where would you find the following:
Tinea unguium
Definition
Nails
Term
Where would you find the following:
Tinea cruris
Definition
Groin
Term
Where would you find the following:
Tinea pedis
Definition
Feet
Term
Where would you find the following:
Tinea barbae
Definition
Beard
Term
Dermatophytes: Hair - Certain fungi produce _____ which under Woods lamp fluoresces
Definition
Metabolite (pteridine)
Term
What is characteristic of Microsporum infection?
Definition
Metabolite (pteridine)
Term
Define the following:
Ectothrix
Definition
Arthroconidia found outside of hair shaft
Term
Define the following:
Endothrix
Definition
Arthroconidia found inside hair shaft
Term
Define the following:
Favus
Definition
Septate hyphae in hair
Term
What 2 types of media would you use for a Dermatophyte?
Definition
Dermatophyte test medium (DTM)
Sabouraud dextrose agar w/antibiotics
Term
How long do you incubate DTM?
Definition
14 days
Term
At what temperature do you incubate DTM?
Definition
RT
Term
DTM - Yellow indicator becomes _____ if dermatophyte grows
Definition
Red
Term
What can DTM inhibit?
Definition
Bacteria & Some Saprophytic Molds
Term
What are the 2 antibiotics associated with Sabouraud dextrose agar?
Definition
Cycloheximide
Chloramphenicol
Term
Describe the Macroconidia if present for Trichophyton
Definition
Smooth/Pencil thin, long, septate
Term
In general how can you ID Trichophyton?
Definition
Use Vitamin Requirements to ID
Term
What type of media should you use for Trichophyton?
Definition
Agar +/= specific nutrient thiamine/casein/inositol
Term
Explain the Hair Perforation Test to ID T. mentagrophytes
Definition
1. Expose hair filaments to dermatophyte for 4 weeks
2. Examine with LPCB
3. Examine for wedge shaped perforations
Term
Will T. mentagrophytes be positive or negative for wedge shaped perforations in the hair perforation test?
Definition
Positive
Term
Which MO is associated with Black dot ringworm?
Definition
T. tonsurans
Term
What is the leading in children?
Definition
T. tonsurans
Term
Which Trichophyton is associated with the following:
Causes a variety of lesions in cattle & humans
Most often seen in farmers
Definition
T. verrucosum
Term
Which Trichophyton is associated with the following:
Produces a black dot type of tinea capitis
Violet Reverse side of colony
Definition
T. violaceum
Term
What is the color of the reverse side of the T. violaceum species?
Definition
Violet
Term
What does T. schoenleinii cause?
Definition
Favus
Term
Describe a Favic type of hair infection
Definition
Hyphae inside hair shaft
Term
Are Microconidia rare or very much present in T. schoenleinii?
Definition
Rare
Term
Describe the appearance of the hyphae of T. schoenleinii
Definition
Knobby & club-shaped at terminal end
Term
What morphology is characteristic of T. schoenleinii?
Definition
Antler hyphae or Favic chandeliers
Term
Describe the Macroconidia of Microsporum
Definition
Large
Spindle-shaped
Rough-walled
Thick walls
Term
Describe the Microconidia of Microsporum
Definition
Rare
Term
Which MO is the most common cause of ringworm in dogs & cats in the U.S.A.?
Definition
M. canis
Term
What is the color of the Reverse of colony of M. canis?
Definition
Bright Yellow
Term
Describe the Macroconidia of M. canis
Definition
Spindle-shaped with tapered ends
Echinulate (warty projections)
Term
How do you usually ID M. audouinii
Definition
Exclusion of other dermatophytes
Term
True or False:
M. audouinii Conidia are rare and the Macroconidia, if present, are bizarre shaped
Definition
True
Term
True or False:
M. gypseum rarely causes human/animal infection
Definition
True
Term
Where can M. gypseum be found?
Definition
In the soil
Term
Describe the Macroconidia of M. gypseum
Definition
Spindle-shapes macroconidia
Ends NOT Tapered
Term
What is the one species of Epidermophyton?
Definition
E. floccosum
Term
What is E. floccosum sensitive to?
Definition
The cold
Term
What should you do/not do for Epidermophyton specimens submitted for dermatophyte culture?
Definition
NOT Refrigerate
Term
Describe the Microconidia of Epidermophyton
Definition
Absent
Term
Describe the Macroconidia of Epidermophyton / E. floccosum
Definition
Beaver-Tail (Club-shaped)
Rounded at tip
Thin-Walled
Multiseptate
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