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Exam 5 Mycobacterial Culture Summery Table
Table
28
Biology
Undergraduate 3
04/11/2015

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Term
Given the name of the media, Determine what type of media it is:
Petragnani
Definition
Egg-Based Nonselective
Term
Given the name of the media, Determine what type of media it is:
American Thoracic Society
Definition
Egg-Based Nonselective
Term
Given the name of the media, Determine what type of media it is:
Lowenstein-Jensen
Definition
Egg-Based Nonselective
Term
Given the name of the media, Determine what type of media it is:
Gruft modification of LJ
Definition
Egg-Based Selective
Term
Given the name of the media, Determine what type of media it is:
Mycobactosel LJ
Definition
Egg-Based Selective
Term
Given the name of the media, Determine what type of media it is:
7H10 agar
Definition
Middlebrook Agar Nonselective
Term
Given the name of the media, Determine what type of media it is:
7H11 agar
Definition
Middlebrook Agar Nonselective
Term
Given the name of the media, Determine what type of media it is:
Mitchison's selective 7H11
Definition
Middlebrook Agar Selective
Term
Given the name of the media, Determine what type of media it is:
Mycobactosel-Middlebrook 7H10
Definition
Middlebrook Agar Selective
Term
Given the name of the media, Determine what type of media it is:
7H9 (used by many commerical AFB systems)
Definition
Broth
Term
Given the name of the media, Determine what type of media it is:
7H12
Definition
Broth
Term
Given the name of the media, Determine what type of media it is:
7H13
Definition
Broth
Term
Given the name of the media, Determine what type of media it is:
Dubos Tween albumin
Definition
Broth
Term
What are the M. haemophilum medias?
Definition
CHOC, BAP, LJ with ferric ammonium citrate, or supplemente Middlebrook 7H10
Term
Which organism requires hemin, hemoglobin, or ferric ammonium citrate?
Definition
M. haemophilum
Term
What type of atmosphere improves recovery of a Mycobacterial organism?
Definition
CO2
Term
Middlebrook media must be incubated in what type of atmosphere?
Definition
CO2
Term
What type of lighting condition should Mycobacterial cultures be put in?
Definition
Protect from light
Term
What does Middlebrook media form in light?
Definition
Formaldehyde
Term
At what temperature are Mycobacterial cultures generally incubated in?
Definition
37 degrees Celsius
Term
At what temperature are Mycobacterial cultures incubated in if M. haemophilum, M. marinum, and/or M. ulcerans is/are suspected?
Definition
25 to 33 degrees Celsius
Term
For how long are Mycobacterial cultures incubated for?
Definition
8 weeks
Term
True or False:
You can incubate Mycobacterial cultures for 6 weeks for some commercial AFB systems
Definition
True
Term
What does the following Commercial System Measure?
BACTEC 460
Definition
14CO2 production
Term
What does the following Commercial System Measure?
BACTEC 9000
Definition
O2 changes using a fluorescent sensor
Term
What does the following Commercial System Measure?
MB/BacT
Definition
CO2 production with CO2 sensor & colorimetric sensor
Term
What does the following Commercial System Measure?
ESP Myco System
Definition
Pressure changes (manometric system)
Term
What is the following Commercial System used for?
Septi-Chek AFB
Definition
Biphasic Medium
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