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Exam 5 Acid-Fast Stain Summary & T. rubrum v. mentagrophytes
Tables
30
Biology
Undergraduate 3
04/11/2015

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Term
What is the Growth Rate of the Following:
T. rubrum
Definition
Slow
Term
What is the Growth Rate of the Following:
T. mentagrophytes
Definition
Intermediate
Term
What is the Colony Morphology (Reverse side) of the Following:
T. rubrum
Definition
Cherry-Red
Term
What is the Colony Morphology (Reverse side) of the Following:
T. mentagrophytes
Definition
Rose Brown
Term
What is the Microconidia of the following:
T. rubrum
Definition
Tear-drop
"Birds on the fence"
Term
What is the Microconidia of the following:
T. mentagrophytes
Definition
Grapelike clusters
Term
What is the Urease Result of the following:
T. rubrum
Definition
Negative
Term
What is the Urease Result of the following
T. mentagrophytes
Definition
Positive
Term
What is the Hair Perforation Test Result of the following
T. rubrum
Definition
Negative
Term
What is the Hair Perforation Test Result of the following:
T. mentagrophytes
Definition
Positive
Term
True or False:
Fluorescent stain is more sensitive than the carbolfuchsin stains
Definition
True
Term
True or False:
Rapid growers may stain with the carbolfuchsin stains but not the fluorescent stain
Definition
True
Term
What is the Decolorizer used with Ziehl-Neelson, Kinyoun, and Fluorochrome?
Definition
Acid Alcohol
Term
What is the Counterstain used with Ziehl-Neelson & Kinyoun?
Definition
Methylene Blue
Term
What is the Counterstain used with Fluorochrome?
Definition
Potassium permanganate
Term
What does Potassium permanganate do with the Fluorochrome stain method?
Definition
Quences background
Term
What type of microscope and at what magnification do you use for the following:
Ziehl-Neelson
Definition
Light Microscope
Use oil immersion objective
Term
What type of microscope and at what magnification do you use for the following:
Kinyoun
Definition
Light Microscope
Use oil immersion objective
Term
What type of microscope and at what magnification do you use for the following:
Fluorochrome
Definition
Fluorescent Microscope
Use High Dry Objective
Term
What color is associated with a Positive Appearance when using the Ziehl-Neelson or Kinyoun stain?
Definition
Red
Term
What color is associated with a Negative Appearance when using the Ziehl-Neelson or Kinyoun stain?
Definition
Blue
Term
What color is associated with a Positive Appearance when using the Fluorochrome stain?
Definition
Yellow or Yellow-Orange
Term
What color is associated with a Negative Appearance when using the Fluorochrome stain?
Definition
No fluorescing organisms
Term
Given the Staining Agent give the Stain Method:
Carbolfuchsin with phenol
Slides with stain are heated to force carbolfuchsin into AFB cell walls
Definition
Ziehl-Neelson
Term
Given the Staining Agent give the Stain Method:
Carbolfuchsin with phenol (High concentrations)
Heat not needed
Definition
Kinyoun stain
Term
Given the Staining Agent give the Stain Method:
Auramine O (+/= rhodamine) with phenol
Definition
Fluorochrome stain
Term
Of the most commonly isolated mycobacteria, which is isolated approximately 3-5% of the time?
Definition
M. TB
Term
Of the most commonly isolated mycobacteria, which is isolated approximately 85-87% of the time?
Definition
M. Avium Complex
Term
Of the most commonly isolated mycobacteria, which is isolated approximately 10% of the time?
Definition
M. fortuitum-chelonae-abscessus
Term
What 2 agents are used for Decontamination/concentration of Mycobacterial samples?
Definition
N-acetyl-1-cysteine
2% NaOH
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