Shared Flashcard Set

Details

Exam 3 - Vibrio, Campylobacter, and Related MOs
Exam 3 - Vibrio, Campylobacter, and Related MOs
73
Biology
Undergraduate 3
03/07/2015

Additional Biology Flashcards

 


 

Cards

Term
What is the gram stain for Vibrio?
Definition
GNB
Term
What is the gram stain for Campylobacter?
Definition
GNB
Term
Is Vibrio species Oxidase positive or negative?
Definition
Positive
Term
Is Campylobacter species Oxidase positive or negative?
Definition
Positive
Term
Which serotypes of Vibrio cholerae are known to cause epidemic cholera?
Definition
O1
O139
Term
What is the normal habitat for Vibrio species?
Definition
Aquatic environments
Term
True or False:
Most Vibrios are halophilic
Definition
True
Term
Is V. cholerae halophilic?
Definition
No
Term
True or False:
All vibrios can grow in low (1%) NaCl
Definition
True
Term
True or False:
Most Vibrio species are motile
Definition
True
Term
Is the following intestinal, extraintestinal, or both?
V. cholerae
Definition
Both Intestinal & Extraintestinal
Term
Is the following intestinal, extraintestinal, or both?
V. parahaemolyticus
Definition
Both Intestinal & Extraintestinal
Term
Is the following intestinal, extraintestinal, or both?
V. vulnificus
Definition
Both Intestinal & Extraintestinal
Term
Is the following intestinal, extraintestinal, or both?
V. alginolyticus
Definition
Extraintestinal
Term
How are Vibrios generally transmitted?
Definition
Eating contaminated food (especially seafood) or water.
Contact with water
Term
Which MO will cause the patient to have Rice-water stool?
Definition
Vibrio cholerae
Term
What does the Vibrio cholerae toxin stimulate the secretion of?
Definition
Water
Electrolytes
Term
Epidemics of Cholera are usually caused by what serotype of V. cholerae?
Definition
O1
Term
True or False:
Most Vibrio species will grow on BAP
Definition
True
Term
True or False:
Most Vibrio species will grow on MAC
Definition
True
Term
True or False:
Cholera-like diarrhea (milder) is usually by non-O1 and non-0139 V. cholerae
Definition
True
Term
True or False:
Extraintestinal infections due to V. cholerae(Bacteremia, wound infections, and ear infections) are usually caused by non-O1 in immunocompromised patients
Definition
True
Term
Which of the Vibrios that we went over in class are probably the least pathogenic?
Definition
V. alginolyticus
Term
What type of diarrhea is most commonly due to V. parahaemolyticus?
Definition
Watery Diarrhea
Term
What type of Vibrio species causes Primary septicemia through gastrointestinal route, wound infections, and gastoenteritis
Definition
V. vulnificus
Term
Patients who have V. vulnificus often have what types of underlying disease?
Definition
Liver
Term
Does V. vulnificus have a High or Low Morality rate?
Definition
High Morality
Term
What are the 2 appropriate specimens for Vibrio specimen collection?
Definition
Fecal specimens & other infected body sites
Term
True or False:
Transport media is often needed for Vibrio species because they are very susceptible to damage
Definition
True
Term
What type of motility is characteristic of V. cholerae?
Definition
Darting or Shooting-Star
Term
True or False:
When Vibrio species are cultured to other enteric media (EMB, SS) there may be growth, but it will be poor if at all
Definition
True
Term
TCBS Agar is selective and differential for what type of MO?
Definition
Vibrio
Term
Of the Vibrios covered in class, what type are sucrose fermenters and have yellow colonies?
Definition
V. cholerae
V. alginolyticus
Term
Of the Vibrios covered in class, what type are sucrose nonfermenters and have blue-green colonies?
Definition
V. vulnificus
V. parahaemolyticus
Term
APW, Alkaline Peptone Water, is an enrichment media for what type of MO?
Definition
Vibrio
Term
What will you subculture APW to?
Definition
TCBS
Term
0/129 is a susceptibility test for what type of MO?
Definition
Vibrio
Term
True or False:
V. vulnificus is susceptible to both the 10 micrograms disk and 150 micrograms disk of 0/129 run on heart infusion agar
Definition
False
Term
True or False:
V. cholera is susceptible to both the 10 micrograms disk and 150 micrograms disk of 0/129 run on heart infusion agar
Definition
True
Term
Vibrio colonies are usually present after incubation overnight at what conditions?
Definition
35 degrees Celsius
Air
Term
What type of MOs string when mixed with 0.5% bile?
Definition
Vibrio
Term
True or False:
All Vibrios ferment glucose
Definition
True
Term
Identify the following:
Oxidase +
Yellow colonies on TCBS
Agglutination with O1 antiserum
Definition
Presumptive V. cholerae
Term
What is the gram stain for Aeromonas species?
Definition
GNB
Term
What is the habitat of Aeromonas species?
Definition
Fresh and Salt Water
Term
How are Aeromonas species transmitted?
Definition
contaminated food, water, or cold-blooded animals
Term
What are diseases commonly caused by Aeromonas species?
Definition
Gastroenteritis & extraintestinal infections; wounds usually associated with trauma and water
Term
Are Aeromonas species Oxidase Positive or Negative?
Definition
Positive
Term
True or False:
All Aeromonas species ferment glucose (some also ferment lactose).
Definition
True
Term
Are Aeromonas species halophilic or nonhalophilic?
Definition
Non-halophilic
Term
What is the Gram Stain for Campylobacter?
Definition
Curved, GNB
Term
What is the characteristic shape of Campylobacter species?
Definition
S-shape, "Seagull-wing", "fingernail clippings", spirals
Term
Are Campylobacter species aerobic or anaerobic?
Definition
Microaerobic
Term
Are Campylobacter species Oxidase Positive or Negative?
Definition
Positive
Term
Are Campylobacter species Motile?
Definition
Yes
Term
What is the characteristic motility for Campylobacter?
Definition
Darting
Term
How is Campylobacter species transmitted?
Definition
Contaminated food, especially poultry and dairy products.
Animal contact
Term
What is the leading cause of diarrhea worldwide?
Definition
Gastroenteritis
Term
Name the 2 media that are selective for Campylobacter species
Definition
CAMPY-BAP
Skirrow medium
Term
The following is characteristic of what type of environment:
85% N2
5% O2
10% CO2
Definition
Microaerobic
Term
Identify the following:
Oxidase +
Catalase +
Growth at 42 degrees Celsius
Darting motility
Definition
Presumptive Campylobacter species
Term
Identify the following:
Cephalothin - Resistant
Nalidixic Acid - Susceptible
Hippurate - Negative
Definition
Campylobacter coli
Term
Identify the following:
Cephalothin - Resistant
Nalidixic Acid - Susceptible
Hippurate - Positive
Definition
Campylobacter jejuni
Term
Identify the following:
Cephalothin - Susceptible
Nalidixic Acid - Resistant
Growth @ 25 degrees Celsius - Positive
Definition
Campylobacter fetus
Term
Identify the following:
Cephalothin - Susceptible
Nalidixic Acid - Resistant
Growth @ 25 degrees Celsius - Negative
Definition
Helicobacter pylori
Term
True or False:
C. fetus subsp. fetus won't grow on Campy selective media
Definition
True
Term
What media should you use for C. fetus subsp. fetus?
Definition
BAP or CHOC @ 37 degrees Celsius
Term
Which MO causes gastric ulcers?
Definition
Helicobacter pylori
Term
What is the Gram Stain of Helicobacter?
Definition
GN Spiral shaped
Term
What is the environment needed to culture Helicobacter pylori?
Definition
CHOC
Microaerophilic
37 degrees Celsius
Increased humidity
Term
Identify the following:
Oxidase +
Catalase +
RAPID Urease +
Definition
Helicobacter pylori
Term
How is Helicobacter pylori usually identified?
Definition
Nonculture methods
Term
True or False:
Helicobacter pylori is rarely diagnosed by culture methods
Definition
True
Supporting users have an ad free experience!