Term
What is the Special GS for the following:
Actinomyces |
|
Definition
| Gram positive branching rods (filaments) |
|
|
Term
What is the Special GS for the following:
Capnocytophaga |
|
Definition
|
|
Term
What is the Special GS for the following:
Fusobacterium nucleatum |
|
Definition
|
|
Term
What is the Special GS for the following:
Campylobacter/Helicobacter |
|
Definition
| S-shaped, spirals & seagull wings GNB |
|
|
Term
What is the Special GS for the following:
Gardnerella |
|
Definition
| Gram-variable coccobacilli |
|
|
Term
What is the Special GS for the following:
Cardiobacterium |
|
Definition
|
|
Term
What is the normal habitat of the following:
Leptospira |
|
Definition
|
|
Term
What is the normal habitat of the following:
Bartonella henselae |
|
Definition
|
|
Term
What is the normal habitat of the following:
Pasteurella multocida |
|
Definition
|
|
Term
What is the normal habitat of the following:
Capnocytophaga canimorsus/cynodegmi |
|
Definition
|
|
Term
What is the normal habitat of the following:
Spirillium minor & Streptobacillus moniliformis |
|
Definition
|
|
Term
What is the normal habitat of the following:
Brucella |
|
Definition
|
|
Term
What is the normal habitat of the following:
Campylobacter |
|
Definition
|
|
Term
What is the normal habitat of the following:
Franciscella tularemia |
|
Definition
|
|
Term
What is the normal habitat of the following:
Coxiella |
|
Definition
|
|
Term
What is the normal habitat of the following:
Rickettsia/Ehrlichia/Anaplasma/Borrelia burgdorferi |
|
Definition
|
|
Term
What is the normal habitat of the following:
Borrelia recurrentis |
|
Definition
|
|
Term
What is the normal habitat of the following:
Aeromonas |
|
Definition
|
|
Term
What is the normal habitat of the following:
Legionella |
|
Definition
|
|
Term
What is the normal habitat of the following:
Vibrio |
|
Definition
|
|
Term
What is the normal habitat of the following:
P. aeruginosa |
|
Definition
|
|
Term
| Where is the habitat of HACEK in humans? |
|
Definition
|
|
Term
| Where is the habitat of Capnocytophaga in humans? |
|
Definition
|
|
Term
What is the normal habitat of the following:
Chromobacterium violaceum |
|
Definition
|
|
Term
| Which MO is associated w/ Actinomyces israelii (anaerobe) |
|
Definition
| Aggregatibater actinomycetemcomitans (aerobe) |
|
|
Term
| Which MO is Anaerobic GNB? |
|
Definition
| Bacteroides, Prevotella, Porphyromonas, & Fusobacterium |
|
|
Term
| Which MO is Anaerobic GPC? |
|
Definition
|
|
Term
| Which MO is Anaerobic GNC? |
|
Definition
|
|
Term
| Which MO is Anaerobic Small GPB? |
|
Definition
|
|
Term
| Which MO is Anaerobic Branching GPB? |
|
Definition
|
|
Term
| Which MO is Anaerobic GPB? |
|
Definition
| Eubacterium & Bifidobacterium |
|
|
Term
| Which MO is Anaerobic Box-car GPB? |
|
Definition
|
|
Term
| Which MOs are Microaerobes? |
|
Definition
Campylobacter
Helicobacter |
|
|
Term
| Which MOs are nonfermenters? |
|
Definition
Pseudomonas
Burkholderia
Stenotrophomonas
Acinetobacter
Moraxella |
|
|
Term
Match the following MO with its disease:
Pseudomonas aeruginosa |
|
Definition
| swimmer’s ear, burns, CF patients |
|
|
Term
Match the following MO with its disease:
Burkholderia cepacia |
|
Definition
| CF patients (cepacia syndrome) |
|
|
Term
Match the following MO with its disease:
Burkholderia pseudomallei |
|
Definition
|
|
Term
Match the following MO with its disease:
Vibrio cholerae |
|
Definition
|
|
Term
Match the following MO with its disease:
Helicobacteria pylori |
|
Definition
|
|
Term
Match the following MO with its disease:
Campylobacter fetus subsp fetus |
|
Definition
| bacteremia in immunocompromised patients |
|
|
Term
Match the following MO with its disease:
Campylobacter jejuni & C. coli |
|
Definition
| usually intestinal (diarrhea) |
|
|
Term
Match the following MO with its disease:
Legionella |
|
Definition
| Pontiac fever/Legionnaire’s |
|
|
Term
Match the following MO with its disease:
Brucella |
|
Definition
| brucellosis or undulant fever |
|
|
Term
Match the following MO with its disease:
Kingella kingae |
|
Definition
| joint infection in young children |
|
|
Term
Match the following MO with its disease:
Franciscella tularemia |
|
Definition
| tularemia or rabbit fever |
|
|
Term
Match the following MO with its disease:
HACEK |
|
Definition
|
|
Term
Match the following MO with its disease:
Bordetella |
|
Definition
| pertussis (whooping cough) |
|
|
Term
Match the following MO with its disease:
Gardnerella vaginalis |
|
Definition
|
|
Term
Match the following MO with its disease:
Clostridium perfringens |
|
Definition
|
|
Term
Match the following MO with its disease:
Clostridium difficile |
|
Definition
| antibiotic associated diarrhea/pseudomembranous colitis |
|
|
Term
Match the following MO with its disease:
Clostridium septicum |
|
Definition
| bacteremia associated w/ underlying malignancy |
|
|
Term
Match the following MO with its disease:
Clostridium tetani |
|
Definition
|
|
Term
Match the following MO with its disease:
Clostridium botulinum |
|
Definition
|
|
Term
Match the following MO with its disease:
Mycoplasma pneumoniae |
|
Definition
| primary atypical pneumonia |
|
|
Term
Match the following MO with its disease:
Mycoplasma hominis |
|
Definition
|
|
Term
Match the following MO with its disease:
Ureaplasma |
|
Definition
|
|
Term
Match the following MO with its disease:
Chlamydia trachomatis |
|
Definition
| trachoma, cervicitis, urethritis, inclusion conjunctivitis, neonatal pneumonia, lymphogranuloma venereum |
|
|
Term
Match the following MO with its disease:
Spirillium minor & Streptobacillus moniliformis |
|
Definition
|
|
Term
Match the following MO with its disease:
Leptospira |
|
Definition
|
|
Term
Match the following MO with its disease:
Borrelia recurrentis |
|
Definition
|
|
Term
Match the following MO with its disease:
Treponema pallidum |
|
Definition
|
|
Term
Match the following MO with its disease:
Rickettsia rickettsii |
|
Definition
| Rocky Mountain spotted fever |
|
|
Term
Match the following MO with its disease:
Ehrlichia chaffeensis |
|
Definition
| Human monocytic ehrlichiosis |
|
|
Term
Match the following MO with its disease:
Anaplasma phagocytophilum |
|
Definition
| Human granulocytic anaplasmosis |
|
|
Term
Match the following MO with its disease:
Coxiella burnetii |
|
Definition
|
|
Term
Match the following MO with its disease:
Bartonella quintana |
|
Definition
| trench fever, bacteremia, bacillary angiomatosis |
|
|
Term
Match the following MO with its disease:
Bartonella henselae |
|
Definition
| cat scratch fever, bacteremia, bacillary angiomatosis |
|
|
Term
| What MO needs epithelial cells? |
|
Definition
|
|
Term
What MO(s) is/are attributed to the following special patient characteristic(s):
Cystic fibrosis |
|
Definition
| Burkholderia cepacia; also mucoid P. aeruginosa |
|
|
Term
What MO(s) is/are attributed to the following special patient characteristic(s):
Dogs |
|
Definition
| Bartonella henselae, Pasteurella multocida, & Capnocytophaga canimorsus/cynodegmi |
|
|
Term
What MO(s) is/are attributed to the following special patient characteristic(s):
Ingestion of contaminated seafood/water |
|
Definition
|
|
Term
What MO(s) is/are attributed to the following special patient characteristic(s):
History of liver disease |
|
Definition
|
|
Term
What MO(s) is/are attributed to the following special patient characteristic(s):
Joint infection in young children |
|
Definition
|
|
Term
| Which MO needs to be transported on wet ice? |
|
Definition
|
|
Term
| Which MO(s) need(s) to be transported in special transport media? |
|
Definition
|
|
Term
What is the appropriate/available test for the following MO(s):
Legionella, Francisella, C. trachomatis, & R. rickettsii |
|
Definition
|
|
Term
What is the appropriate/available test for the following MO(s):
Legionella (urine) |
|
Definition
|
|
Term
What is the appropriate/available test for the following MO(s):
Brucella - nonautomated: hold broth cultures 30 days & automated broth cultures hold 10 days |
|
Definition
|
|
Term
What is the appropriate/available test for the following MO(s):
Bordetella & Ehrlichia/Anaplasmosis |
|
Definition
|
|
Term
What is the appropriate/available test for the following MO(s):
C. difficile & C. botulinum |
|
Definition
|
|
Term
What is the appropriate/available test for the following MO(s):
Vibrio 01 & 0139, Francisella, Brucella, Heliobacter, Coxiella, & Bartonella |
|
Definition
|
|
Term
What is the appropriate/available test for the following MO(s):
Mycoplasma pneumoniae |
|
Definition
|
|
Term
What is the appropriate/available test for the following MO(s):
T. pallidum |
|
Definition
|
|
Term
What is the appropriate/available test for the following MO(s):
Gardnerella vaginalis |
|
Definition
|
|
Term
True or False:
The following are considered as Biohazardous:Brucella & Franciscella tularemia |
|
Definition
|
|
Term
True or False:
The following need special decontamination: Legionella |
|
Definition
|
|
Term
| What type of special decontamination does Legionella need? |
|
Definition
|
|
Term
True or False:
Thios need to be boiled before being used |
|
Definition
|
|
Term
| What is the appearance of nonfermenters on MAC/EMB? |
|
Definition
|
|
Term
What does the following MO look like on the following media?
B. fragilis
BBE |
|
Definition
|
|
Term
What does the following MO look like on the following media?
Bordetella
Bordet-Gengou/charcoal horse blood agar (Regan-Lowe) |
|
Definition
|
|
Term
What does the following MO look like on the following media?
Gardnerella
Human blood agar (V agar or HBT agar) |
|
Definition
|
|
Term
What does the following MO look like on the following media?
Clostridium difficile
CCFA |
|
Definition
|
|
Term
What does the following MO look like on the following media?
B. cepacia
BCSA/OFPBL |
|
Definition
|
|
Term
What does the following MO look like on the following media?
V. cholerae/alginolyticus
TCBS |
|
Definition
|
|
Term
What does the following MO look like on the following media?
V. vulnificus/parahaemolyticus
TCBS |
|
Definition
|
|
Term
What is the incubation temperature that is needed for the following:
C for Campylobacter jejuni & C. coli |
|
Definition
|
|
Term
What is the incubation temperature that is needed for the following:
C. fetus & H. pylori |
|
Definition
|
|
Term
What is the incubation environment that is needed for the following:
HACEK group, Capnocytophaga, & Brucella |
|
Definition
|
|
Term
What is the incubation environment that is needed for the following:
Campylobacter & Helicobacter |
|
Definition
|
|
Term
What is the incubation time that is needed for the following:
Anaerobes in GasPak containers at 350 |
|
Definition
| 48 hours before reading plates |
|
|
Term
| How are Chlamydia MOs detected? |
|
Definition
Iodine stain for glycogen inclusion
DFA (monoclonal ABY) |
|
|
Term
| What is the best method for detection for Chlamydia? |
|
Definition
|
|
Term
| What else must be done for detection of Chlamydia if it is a child abuse case? |
|
Definition
|
|
Term
| What type of cells and media are used for a tissue culture for detection of Chlamydia? |
|
Definition
|
|
Term
What disease/illness are attributed to the following MO:
C. pneumonia |
|
Definition
|
|
Term
What disease/illness are attributed to the following MO:
C. psittaci |
|
Definition
|
|
Term
True or False:
Tissue cultures and DFA are commonly used when identifying C. psittachi |
|
Definition
|
|
Term
| Weyant and Associates charts identify MOs on what grounds? |
|
Definition
| Oxidase, growth on MAC, OF results, & motility |
|
|
Term
| Name 4 OF Glucose Fermenters |
|
Definition
| Enterics, Vibro, Aeromonas, & Plesiomonas |
|
|
Term
| Name 1 OF Glucose Oxidizer |
|
Definition
|
|
Term
OF Glucose Test:
Both Tubes Yellow |
|
Definition
|
|
Term
OF Glucose Test:
Yellow in open
Blue-Green in closed |
|
Definition
|
|
Term
OF Glucose Test:
Both Tubes Blue-Green |
|
Definition
| Non-Oxidizer/Assachrolytic |
|
|
Term
|
Definition
| Moraxella & Acinetobacter |
|
|
Term
What type of MO has the following Nitrate test results?
red after adding reagents
A & B (nitrate → nitrite) |
|
Definition
|
|
Term
What type of MO has the following Nitrate test results?
No color change after addition of zinc (nitrate → nitrogen gas) |
|
Definition
|
|
Term
What type of MO has the following Nitrate test results?
red color after addition of zinc (nitrate not reduced) |
|
Definition
|
|
Term
Identify the MO with the following results:
Pyocyanin production (green); oxidase +
Growth at 42; TSI = K/K
OF Glucose = oxidizes |
|
Definition
|
|
Term
Identify the MO with the following results:
SPS = S |
|
Definition
| Peptostreptococcus anaerobius |
|
|
Term
Identify the MO with the following results:
Kana, Vanco, Col, & Bile = R
BBE = black (positive) |
|
Definition
|
|
Term
Identify the MO with the following results:
Clue cells on GS or wet mount
If cultured – β-hemolytic on HBT or V agar; γ-hemolytic on BAP
Starch + |
|
Definition
|
|
Term
Identify the MO with the following results:
Double zone of hemolysis
Reverse CAMP = +
Lecithinase = + |
|
Definition
|
|
Term
Identify the MO with the following results:
Grows on BAP & CHOC at 350
Rapid urease = +
Usually diagnosed by serology, direct histologic examination or urease production |
|
Definition
|
|
Term
Determine whether the following is Oxidase Positive or Oxidase Negative:
Acinetobacter |
|
Definition
|
|
Term
Determine whether the following is Oxidase Positive or Oxidase Negative:
Stenotrophomonas |
|
Definition
|
|
Term
Determine whether the following is Oxidase Positive or Oxidase Negative:
Plesiomonas |
|
Definition
|
|
Term
Determine whether the following is Oxidase Positive or Oxidase Negative:
Aeromonas |
|
Definition
|
|
Term
Determine whether the following is Oxidase Positive or Oxidase Negative:
Campy & Helico |
|
Definition
|
|
Term
Determine whether the following is Oxidase Positive or Oxidase Negative:
Vibrio |
|
Definition
|
|
Term
Determine whether the following is Oxidase Positive or Oxidase Negative:
Burkholderia |
|
Definition
|
|
Term
Determine whether the following is Oxidase Positive or Oxidase Negative:
Moraxella |
|
Definition
|
|
Term
Determine whether the following is Oxidase Positive or Oxidase Negative:
Kingella |
|
Definition
|
|
Term
Determine whether the following is Oxidase Positive or Oxidase Negative:
Eikenella |
|
Definition
|
|
Term
| Is V. cholerae halophilic or nonhalophilic? |
|
Definition
|
|
Term
| Except V. cholerae, are most of the other vibrios halophilic or nonhalophilic? |
|
Definition
|
|
Term
| Which MO appears green on MH agar due to pyocyanin? |
|
Definition
|
|
Term
What is the order for the following MO:
Eikenella |
|
Definition
|
|
Term
What is the order for the following MO:
Pseudomonas aeruginosa |
|
Definition
|
|
Term
What is the order for the following MO:
Myroides odoratum |
|
Definition
|
|
Term
What is the order for the following MO:
Anaerobes |
|
Definition
|
|
Term
Which MO has the following color:
Yellow |
|
Definition
| Flavobacteria & Capnocytophaga |
|
|
Term
Identify the MO that is attributed to the following key characteristic:
Mercury drop colonies |
|
Definition
|
|
Term
Identify the MO that is attributed to the following key characteristic:
Pitting agar |
|
Definition
|
|
Term
Identify the MO that is attributed to the following key characteristic:
Molar tooth colony |
|
Definition
|
|
Term
Identify the MO that is attributed to the following key characteristic:
Sulfur granules |
|
Definition
|
|
Term
Identify the MO that is attributed to the following key characteristic:
Double zone of β-hemolysis |
|
Definition
|
|
Term
Identify the MOs that are attributed to the following key characteristic:
Fluorescence red (GNB) |
|
Definition
| Prevotella & Porphyromonas |
|
|
Term
Identify the MO that is attributed to the following key characteristic:
Fluorescence red (GNC) |
|
Definition
|
|
Term
Identify the MOs that are attributed to the following key characteristic:
darting motility |
|
Definition
| V. cholerae & Campylobacter |
|
|
Term
Identify the MO that is attributed to the following key characteristic:
gliding motility |
|
Definition
|
|
Term
Identify the MO that is attributed to the following key characteristic:
SXT Sensitive |
|
Definition
|
|
Term
Identify the MO that is attributed to the following key characteristic:
Penicillin susceptibility |
|
Definition
|
|
Term
Identify the MO that is attributed to the following key characteristic:
Fried-egg colonies |
|
Definition
|
|
Term
Identify the MO that is attributed to the following key characteristic:
Anaerobic diphtheroids |
|
Definition
|
|
Term
Identify the MO that is attributed to the following key characteristic:
Rice water stool |
|
Definition
|
|
Term
What color does the following indicator strip turn when O2 is present?
Methylene Blue |
|
Definition
|
|
Term
What color does the following indicator strip turn when O2 is present?
Resazurin |
|
Definition
|
|
Term
| How long does it take to achieve anaerobic conditions in an anaerobe jar? |
|
Definition
|
|
Term
| How long do you need to incubate in a GasPak EZ container before opening? |
|
Definition
|
|
Term
| What is the procedure to ID if anaerobe using the Aero-tolerance Test? |
|
Definition
Subculture colonies to CHOC & ANA-BAP
Incubate CHOC in CO2 & ANA-BAP in anaerobic environment
After 2 days check plates for growth of using jars; next day if using glove box |
|
|
Term
| When dealing with anaerobes what type of instrument should you use? |
|
Definition
| platinum, stainless steel loop, or sterile wooden stick |
|
|
Term
| How do you/What do you use to collect anaerobe specimens? |
|
Definition
| Aspirates/Tissues are Great |
|
|
Term
| What is OK to use to collect anaerobe specimens? |
|
Definition
|
|
Term
What type of specimens are the following considered when referring to anaerboes?
Throat/nasopharyngeal/sputum
Feces or bowel contents
Voided urine
Vaginal/cervical swabs |
|
Definition
| Inappropriate specimens (with normal flora) |
|
|
Term
True or False:
Anaerobes - Use special transport media or specimen has to be brought to lab immediately after collection |
|
Definition
|
|
Term
True or False:
Anaerobes - If color (resazurin turns pink) in transport media, it is unacceptable for use |
|
Definition
|
|
Term
| C. difficile is usually diagnosed by ... |
|
Definition
| detecting toxins produced by organism |
|
|
Term
True or False:
The cytotoxicity test was once considered the “gold standard” & is now being replaced by PCR |
|
Definition
|
|
Term
True or False:
The cytotoxicity test is performed in tissue cells |
|
Definition
|
|
Term
| What do the components of the cytotoxicity test include? |
|
Definition
| stool (patient), tissue cells, & anti-toxin |
|
|
Term
Interpret the following:
Stool + Tissue Cells (Human fibroblasts) --> NO CPE |
|
Definition
| No C. difficile toxin present |
|
|
Term
Interpret the following:
Stool + Tissue Cells (Human fibroblasts) --> CPE |
|
Definition
| C. difficile toxin present |
|
|
Term
Interpret the following:
Stool + Antitoxin + Tissue Cells (Human fibroblasts) --> NO CPE |
|
Definition
| C. difficile toxin present |
|
|
