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Exam 3 - Miscellaneous Organisms
Exam 3 - Miscellaneous Organisms
81
Biology
Undergraduate 3
03/08/2015

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Term
True or False:
Mycoplasma/Ureaplasma won't stain with gram stain
Definition
True
Term
What MO causes Primary Atypical Pneumonia?
Definition
Mycoplasma pneumoniae
Term
What MO is characterized by "Fried Egg" colonies?
Definition
Mycoplasma pneumoniae
Term
What MO rises in "Cold agglutinins"?
Definition
Mycoplasma pneumoniae
Term
How do you diagnosis Mycoplasma pneumoniae?
Definition
Serology
PCR
Term
Which MO is tied to Females with postpartum fever?
Definition
Mycoplasma hominis
Term
True or False:
Males with Mycoplasma hominis seldom have illness
Definition
True
Term
Which MO is one of the causes of nongonococcal urethritis in males?
Definition
Ureaplasma urealyticum
Term
True or False:
Ureaplasma urealyticum does not seem to cause illness in females
Definition
True
Term
True or False:
Ureaplasma urealyticum splits urea
Definition
True
Term
Which MO causes Syphilus?
Definition
Treponema pallidum
Term
What are the 2 ways to diagnosis Treponema pallidum?
Definition
Darkfield exam of chancre/rash
Serology
Term
What disease/infection/illness does Leptospira cause?
Definition
Leptospirosis
Term
Where/What is the normal habitat of Leptospira?
Definition
Animal Urine
Term
What is the special media used for suspected Leptospira?
Definition
Fletcher's Agar
Term
What is the incubation environment needed for Leptospira culturing?
Definition
6 weeks in the dark
30 degrees Celsius
Term
What do you use to examine the culture for Leptospira colonies?
Definition
Darkfield Microscopy
Term
Which MO is connected to Relapsing Fever & Louse-borne?
Definition
Borrelia
Term
True or False:
Some species of Borrelia are louse-borne and some species are tick-borne
Definition
True
Term
True or False:
Borrelia is rarely cultured
Definition
True
Term
What is the preferred method of diagnosis for Borrelia?
Definition
Microscopic exam of blood
-may use Giemsa or Wright/darkfield or phase microscopy
Term
With MO is connected to Lyme Disease?
Definition
Borrelia burgdorferi
Term
The following is characteristic of what disease and MO:
Target lesion; Erythema migrans
Definition
Lyme Disease
Borrelia burgdorferi
Term
What is currently the best tool for diagnosis of Lyme Disease?
Definition
Serology
Term
Which MO is connected to Rat Bite Fever?
Definition
Spirillium minus
Term
Which MO, other than Spirillium minus, is connected to Rat Bite Fever?
Definition
Streptobacillus moniliformis
Term
What are the 2 genera of Chlamydiaceae family that we covered in class?
Definition
Chlamydia
Chlamydophila
Term
True or False:
Chlamydiaceae family are intracellular and unable to synthesize ATP
Definition
True
Term
True or False:
Chlamydiaceae family can not be seen by gram stain
Definition
True
Term
The cell wall of the Chlamydiaceae family is similar to that of what type of gram stain?
Definition
GNB
Term
Define Elementary bodies (EB)
Definition
Infectious form of Chlamydia
Term
Define Reticular bodies (RB)
Definition
Non-Infectious form of Chlamydia
Term
What MO(s) is the following the cycle of:
1. EB attaches to the host cell
2. The host cell forms a phagosome & ingest the EB
3. EB reorganizes and forms a RB
4. RB replicated by binary fission
5. RBs reorganize to form EBs
6. The host cell releases the EBs
Definition
Chlamydiaceae family
Term
True or False:
Chlamydia trachomatis is associated almost exclusively with humans
Definition
True
Term
What MO is the following paired with:
Trachoma
-Blindness
-Transmitted by direct contact
Definition
Chlamydia trachomatis
Term
What MO is the following paired with:
Genital - cervicitis, PID, Nongonococcal urethritis
Definition
Chlamydia trachomatis
Term
What MO is the following paired with: Inclusion Conjunctivitis
-Newborns
-Source: Female genital tract
Definition
Chlamydia trachomatis
Term
What MO is the following paired with:
Neonatal pneumonia
Definition
Chlamydia trachomatis
Term
What MO is the following paired with:
Lymphogranuloma venereum (LGV) - Regional lymphadenopathy
Definition
Chlamydia trachomatis
Term
What type of specimen do you need to identify Chlamydia trachomatis?
Definition
Involved epithelial sites
Term
Do you need special transport media for Chlamydia trachomatis?
Definition
Yes
Term
What type of special transport media do you need for Chlamydia trachomatis?
Definition
2SP
Sucrose Phosphate
Term
Does Chlamydia trachomatis need to be kept cold or warm?
Definition
Cold
Term
What type of stain do you use for Chlamydia trachomatis?
Definition
Giemsa stain
Term
You can detect the Chlamydia trachomatis antigen by what method?
Definition
EIA
Term
You must verify the Chlamydia trachomatis antigen by what method?
Definition
Complementary assay
Term
What was once thought to be the Gold Standard for identification of Chlamydia trachomatis?
Definition
Culture
Term
Which MO is cultured with a shell vial and McCoy cells?
Definition
Chlamydia trachomatis
Term
What stain do you use for glycogen inclusion and what MO can you identify?
Definition
Iodine
Chlamydia trachomatis
Term
With MO can you identify with DFA - Monoclonal antibody
Definition
Chlamydia trachomatis
Term
What is the Most sensitive test for detecting Chlamydia trachomatis?
Definition
NAAT
Term
CDC recommends use of NAAT to screen for GC & C. trachomatis; EXCEPT in what type of cases?
Definition
sexual abuse of children
Term
What MO causes 10% of pneumonias worldwide - primarily occurs in children and young adults?
Definition
Chlamydophila pneumonia
Term
Which MO is detected by a tissue culture and visualized with DFA?
Definition
Chlamydophila pneumonia
Term
What is the most common detection method for Chlamydophila pneumonia?
Definition
Serology
Term
What MO causes psittacosis, aka ornithosis aka parrot fever
Definition
Chlamydophila psittaci
Term
What BSL level is Chlamydophila psittaci?
Definition
BSL-3
Term
True or False:
Almost all diagnoses of Chlamydophila psittaci are based on patient history and serology results
Definition
True
Term
What BSL level is Rickettsia rickettsii?
Definition
BSL-3
Term
What direct detection method is the most frequently performed for Rickettsia rickettsii?
Definition
PCR
Term
What serology method do you use for Rickettsia rickettsii?
Definition
Weil-Felix
Term
True or False:
RMSF produces agglutination with Proteus OX-2 & OX-19
Definition
True
Term
True or False:
Specific antibodies for Rickettsia rickettsii may not appear for 1-2 weeks
Definition
True
Term
Which serology method is the most sensitive and specific for Rickettsia rickettsii?
Definition
Indirect Immunofluorescence (IFA)
Term
How are Ehrlicia/Anaplasma transmitted?
Definition
Ticks
Term
Which MO is associated with Human monocytic ehrlichiosis, also known as "Rashless RMSF"?
Definition
Ehrlichia chaffeensis
Term
Which MO causes Hhuman Granulocytic anaplasmosis?
Definition
Anaplasma phagocytophilum
Term
What is the diagnosis method of choice for Ehrlichia/Anaplasma?
Definition
PCR
Term
Which MO causes or is attributed to Q Fever?
Definition
Coxiella burnetii
Term
What is Coxiella burnetii associated with?
Definition
Zoonosis
Slaughterhouses / Animal placentas
Term
How is Coxiella burnetii transmitted?
Definition
Inhalation of infected aerosols
Term
How is Coxiella burnetii diagnosed?
Definition
Serology
Term
Which MO causes or is associated with Trench Fever?
Definition
Bartonella quintana
Term
Which MO is Bacillary angiomatosis - lesions of skin, lymph nodes, and internal organs, in HIV-infected patients associated with?
Definition
Bartonella quintana
Term
Which MO is associated with Cat Scratch Fever?
Definition
Bartonella henselae
Term
Which MO is associated with Bacillary angiomatosis, Bacteremia, and Fever?
Definition
Bartonella henselae
Term
What type of stain is used to diagnosis Bartonella species?
Definition
Warthin-Starry
Term
To culture Bartonella species you incubate in what environment?
Definition
CO2
35 Degrees Celsius
Term
How many day may growth of Bartonella species take?
Definition
9-15 days
Term
True or False:
Bartonella henselae is diagnosed by serologic testing (IFA) + Patient History
Definition
True
Term
What method is used to differentiate Bartonella species?
Definition
PCR
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