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Exam 3
Cellular Division and Inheritance, Genetics, Enzymes, and Cellular Respiration & Photosynthesis
85
Biology
Undergraduate 1
05/01/2014

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Term
Cytokinesis
Definition

The divison of the cytoplasm of a cell after nuclear division. Cytoplasmic division is accomplished by

Cytokinesis.

Term
Chromosome
Definition
Carry the genetic material and this information can be crossed over from generation to generation with no loses and no errors. The prime actors in the division process are the chromsomes, structers made up of protein and DNA that contol cell activities.
Term
Chromatid
Definition
Each chromosome its composed of two parallel strands of equal length held together at some point along their length. These strands are called Chromatids.
Term
Cell Plate
Definition
The structure that forms at the equator of the spindle during early telophase during the dividing of cells of plants  and few green algea.
Term
Tetrad
Definition

Since each chromosome is considered at dyad, each pair of chromosomes contains four chromatids and the joined pair is considered a tetrad. 

The two dyads of a homologous pair of chromosomes joined together during prophase and metaphase of meiosis -- four chromatides and two centromeres -- considered as two chromosomes.

Term
Homologous Chromosome
Definition
Each chromsome should have two chromatids and the chromsomes should be identical in length and in the position of the centromeres making them homologous chromosomes. Pair of the same kind of chromosome in a diaploid cell.
Term
Karyokinesis
Definition
Process of nuclear division. Mitosis and Meiosis are terms that refer to divion of the nucleus or Karyokinesis.
Term
Chromatin
Definition
When the cell is not dividing during interphase, the chromosomes are in the form of chromatin, long thin tangled threads. The chromatin is duplicated once, during interphase.
Term
Centromere
Definition
Each strand of chromatid is held together at some point along their length. The point in which they are joined is called the centromere
Term
Monad
Definition
A chromosome with one single chromatid is a monad, which appear during anaphase after they are seperated and dragged to opposite sides of the poles. Chromosome consisting of a single chromatid and centromere.
Term
Metaphase Plate
Definition
At the end of prophase, the chromosomes are at their shortest and thickest stage. They now line up on a plane equidistant from the two poles. This plane is called the metaphase plate.
Term
Cell Division
Definition
Term
Crossing Over
Definition
During Synapsis, chromosomes may exchange genetic material. This exchange is called crossing-over.
Term
Centriol
Definition
A cytoplasmic organell located outside the nuclear membrane, identical in structure to the basal body: found in animal cells and flagellated cells of other groups; divides and organizes spindle fibers during mitosis and meiosis.
Term
Dyad
Definition
A chromosome consisting of two chromatids helded together by a single centromere is refered to as a dyad. (considered to be one chromosome)
Term
Synapsis
Definition
Early in prophase one during Meiosis, as the chromatin is shortening and thickening into chromosomes, the homologous chromosomes comes together and line up along their entire length. This process is called synapsis.
Term
Sister Chromatides
Definition
The monads in each pair of chromosomes are known as sister chromatids.
Term
The phases of Mitosis and Meosis and the major events of each phase
Definition
Term
Recognize phases of mitosis and meosis from any prepared slides, models
Definition
Term
Be able to compare and contrast mitosis and meiosis
Definition
Term
Be able to apply meiosios to the inheritance of genetic information
Definition
Term
Mitosis
Definition
Is the term applied to the process by which nuclei divide, producing new nuclei identical to the original nucleus
Term
FRUIT FLY GENETICS
Definition
Term
P Generation
Definition
Parents, parental generation. The first generation in a sequence of genetic crosses
Term
F1 and F2 Generation
Definition
"First Filial Generation". The set of offspring produced in the first generation from the parental cross. ("Filial") pertains to sons and daughters.) Similarily F2, F3 . . . Refer to subsequent generations
Term
Gene
Definition
A particular hereditary determiner; a unit of inheritance; a unit of DNA; located in a fixed location (locus) in a chromosome
Term
Locus
Definition
The precise location of a gene on a chromosome (plural = loci)
Term
Allele
Definition
A gene that gives an alternative expression of the same character in conrtrast to other forms of the gene that may appear at a particular locus
Term
Heterozygous
Definition

a condition in which the two genes present at a particular locus are different alleles.

ex. Hh

Term
Homozygous
Definition
condition in which the two genes present at a particular locus are identical (the same allele)
Term
Homozygous Dominant
Definition
Homozygous condition in which both genes at a particular locus are the dominant allele.
Term
Homozygous Recessive
Definition
Homozygous condition in which both genes at a particular locus are the recesive allele.
Term
Geneotype
Definition
The genetic make-up on an individual - shown by symbols
Term
Phenotype
Definition
The physical, behavioral, and chemical forms determined by an individual's gentotype -- described by means of words rather than symbols
Term
Genotypic Ratio
Definition
The relative frequencies of the various possible genotypes to be expected in the offspring of a particular cross
Term
Phenotypic Ratio
Definition
The relative frequencies of the various possible phenotypes to be expected in the offspring of a particular cross.
Term
Monohybrid Cross
Definition
A cross involving a single locus
Term
dihybrid cross
Definition
a cross involving two loci 
( on non-homologous chromosomes)
Term
Dominant
Definition
applied to one memeber of an allelic pair lacking the ability to manifest itself wholly or largely to the exclusion of the expression of the other one
Term
Recessive
Definition
applied to one memeber of an alleic pair lacking the ability to manifest itself when the other or dominant member is present -- produces its phenotype only when homozygous
Term
Diploid
Definition
an organism or cell with two sets of chromosomes, symbolically referred to as "2N" -- in humans, having 46 chromosomes
Term
Haploid
Definition
An organism or cell having only one set of chromomes, symbolically represented as "N" -- in humans, having 23 chromosomes
Term
Gamete
Definition
A mature male or female reproductive cell (sperm or egg)
Term
Know the life cycle of the Drosophila, correctly recognize the phenotypes used in this exersize.
Definition
Term
Be able to correctly formulate null and alternate hypotheses, conduct a chi-square test, determine degrees of freedom, and understand for what type of data this statistical test is appropriate. Know when to accept or reject the null hypothesis in a chi-square test.
Definition
Term
Be able to apply meiosis to the inheritance of genetic information
Definition
Term
ENZYMES
Definition
Term
Active Sites
Definition
The region of an enzyme surface to which a specific set of substrates binds, lowering the activation energy required for a particular chemical reaction and so facilitating it.
Term
Competitive Inhibitor
Definition
An inhibitor that binds to the same active site as an enzymes substrate, there by competeing with the substrate
Term
Enzyme Kinetics
Definition
Term
Enzyme Denaturation
Definition
is a qualification on an enzymatic reaction and the factors that influence the rate of the reaction.
Term
Non-competive/allosteric inhibitor
Definition
An inhibitor that binds to a location other than the active site of an enzyme, changing the enzymes shape so that it cannot bind to the substrate.
Term
What are enzymes and coenzymes? How do they function in cellular metabolism?
Definition
Enzyme is a protein that is capable of speeding up specific chemical reactions by lowering the required activation energy. Coenzyme a non-protein organic molecule such as NAD that plays an accessory role enzyme catalyzed processes, often by acting as a donor or acceptor of electrons.
Term
What is the relationship between an active site and a substrate?
Definition

Substrates bind to the active site

 

Term
What were the enzyme and the substrate used in this expiriment? What was the source of the enzyme and what were the end products?
Definition
Term
Explain the difference between competive and non-competitive inhibition? What is an allosteric site?
Definition
Competitive binds reversably so it cannot be converted to product. Non-competitive binds to a site other than the active site (an allosteric site). When this happens, it decreases the ability of the enzyme to catalyze the raction, thus lowering the reaction velocity.
Term
In this expiriement, what was the function of guaiacol?
Definition
measured the amount of oxygen being produced. Guaiacol is a colorless compund that turns brown in the presence of O2 and can thus be quantified spectromically.
Term
What was the control in this expiriement?
Definition

Guaiacol 0.1ml

Turnip Extract 1.0 ml

pH 7.0 Buffer 8.9 ml

Term

What were (or would be) the effects of and how would you perform the following:

Adding more substrate?

Adding more enzyme?

Changing the temperature?

Changing pH?

Definition
Term
Can you analyze graphs in terms of enzyme activity?
Definition
Term
CELLULAR RESPIRATION AND PHOTOSYNTHESIS
Definition
Term
Catabolic vs. Anabolic
Definition
Term
Anaerobic
Definition

Any process that can occur without oxygen, such as anaerobic fermentation or 

H2S photosynthesis

Term
Chloroplast
Definition
A cell-like organelle present in algea and plants that contain chlorophyll (and usually other pigments) and carries out photosynthesis
Term
Chlorophylls a & b
Definition
The primary type of light absorbing pigment in photosynthesis. Chlorophyll a absorbs light in violet-blue and red ranges of the visible light spectrum; Chlorophyll is an accessory pirment to chlorophyll a, absorbing light in the blue to red-orange range. neither pigment absorbes color in the green ranges.
Term
Thin layer chromatography
Definition
Term
Rf
Definition
Term
Glycolysis
Definition
The Anarobic breakdown of glucose; this enzyme-catalyzed process yeilds two molecules of pyruvate with a net of two molecules of ATP
Term
Aerobic
Definition
Requiring free oxygen; any biological process that can occur in the presence of gaseous oxygen
Term
Grana & Stroma
Definition
Pigements responsible for light absorption as well as the light-dependent reactions are found in the membranes of the chloropast (the grana) and the light-independent reactions are found in the fluid interior of the chloroplast (the stroma)
Term
Carotenoids & Xanthophylls
Definition
Any group of accessory pigments found in plants; in addition to absorbing light energy, these pigments act as antioxidants, scavenging potentialy damaging free radicals. Carontenoids and Xanthophylls are other pigments that participate in photosynthesis.
Term
Fluroescence
Definition
Chlorophyll floreses under ultra violet light
Term
Absorption Spectrum
Definition
The relationship of absorbtin vs. wavelength for a pigment molecule. This indicates which wavelenths are absorbed maximally by a pigment. For example, chlorophyll a absorbes most strongly in the violet-blue and reds of the visible light spectrum
Term
Know the raw materials and end products of gycolysis and aerobic respiration.
Definition
Term
What is facultative anaerobe? An obligate anaerobe?
Definition
Term
What type of respiration did the chick-peas undergo? At what temperature did the respire most rapidly? Why?
Definition
Term
What were the functions of phenolphthalein and sulfuric acid?
Definition
Term
What is the relationship between the amount of carbon dioxide produced and the change in pH?
Definition
Term
Can you calculate the number of micromoles (μmoles) of carbon dioxide produced per ml per hour? How many μmoles of glucose were being consumed per hour by your peas.
Definition
Term
What are the raw materials and end products of photosynthesis? What does photosynthesis accomplish for individual plants and the ecosystem?
Definition
Term
What is the function of chloroplasts and the various pigments in the chloroplast? How did you extract the pigments and measure the absorption spectrum?
Definition
Term
What was the function of CaCO3 in this exersize? What gas production did you measure?
Definition
Term
What part(s) of the visible spectrum is/are absorbed by chlorophyll? Which part(s) is/are absorbed the least (i.e., which is reflected the most)? How do you determine, quantitatively, the answers to the previous two questions?
Definition
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