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Enzymes and Protein Isoforms
CHEM 101
40
Biology
Not Applicable
12/01/2011

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Term
Enzymes
Definition
proteins that are a catalyst of biochemical reactions. Enzymes are not permanently changed by the chemical reactions they catalyze
Term
Substrate
Definition
The reactant that binds to an enzyme and it will be converted into a product.
Term
Product
Definition
The final molecule(s) that result from the enzyme catalyzed biochemical reaction.
Term
Reaction rate (velocity)
Definition
The amount of product produced per unit of time.
Term
Active site
Definition
the region of the enzyme where the substrate binds and the biochemical reaction occurs.
Term
Apoenzyme
Definition
Some enzymes require an additional non-protein prosthetic group to function (same as complex protein). The polypeptide portion of such an enzyme is called the apoenzyme.
Term
Cofactor
Definition
(non-protein portion) are ions or smaller organic molecules needed for the activity of specific enzymes. Some cofactors include metal ions such as Ca2+, Mg2+ and Cu2+.
Term
Coenzyme
Definition
(non-protein portion) are organic molecules derived from niacin, riboflavin, and other water soluble vitamins. They are needed for the activity of specific enzymes.
Term
Holoenzyme
Definition
the complete enzyme-cofactor complex that gives full catalytic activity.
Term
Inhibitor
Definition
materials that reduce the catalytic activity of an enzyme.
Term
Zymogen
Definition
inactive enzyme precursor
Term
Isoenzymes (Isozymes)
Definition
structurally related protein products of different genes that catalyze the same biochemical reaction.
Term
Coupled reaction
Definition
biochemical reactions that share a common intermediate.
Term
Transfer (Transferase)
Definition
catalyzes the transfer of groups such as amino, carboxyl, glucosyl, and methyl. This does not include water.
Term
Redox (Oxidorreductase)
Definition
Catalyze oxidation/reduction reactions.
Term
Hydrolytic (Hydrolase)
Definition
Catalyze hydrolysis reactions.
Term
Ligase
Definition
Catalyzes the covalent bonding of two molecules coupled with the hydrolysis of high energy bonds in ATP or similar compund.
Term
Isomerase
Definition
Catalyzes the introconversion of structures or geometric changes in a molecule.
Term
Lyase
Definition
Catalyzes the formation of double bonds or reactions involving double bonds
Term
Amylase
Definition
digests starches to disaccharides (two simple sugars)
Term
Lipase
Definition
further digests dietary fats into fatty acids and glycerol.
Term
Bile salts
Definition
involved in the emulsification process of lipid absorption.
Term
Peptidases
Definition
can either preak specific peptide bonds depending on the amino acid sequence of a protein, or break down a complete peptide to amino acide.
Term
Sodium bicarbonate
Definition
an alkaline secretion which neutralizes the acitid gastric juice as it enters the small intestine.
Term
Competitive inhibitors
Definition
inhibitors that directly compete with the substrate for binding within the enzyme active site.
Term
Noncompetitive inhibitors
Definition
weakly bind to an allosteric regulatory site on the enzyme such as a cofactor binding site. Does not compete with substrate for active site.
Term
Bilirubin
Definition
the principle pigment in bile derived from the breakdown of hemoglobin.
Term
Cirrhosis
Definition
a complication of many liver diseases that is characterized by abnormal structure and function of the liver.
Term
Excretory
Definition
discharge of waste from an organ (Liver).
Term
Secretory
Definition
process by which substances are released fro an organ for a specific purpose.
Term
Hepatitis
Definition
an inflammation of the liver often caused by infections especially viral, radiation, drugs (medications), chemicals, or toxins.
Term
Jaundice
Definition
the yellowish staining of the skin and sclerae (the whites of the eyes) that is caused by high levels in the blood of bilirubin.
Term
Necrosis
Definition
the death of living cells or tissues
Term
Reticuloendothelial System (RES)
Definition
a group of living cells having the ability to take up and sequester inert particles and vital dyes.
Term
Liver
Definition
Largest gland in the body composed of two main lobes, located in the mid to upper abdominal cavity
Term
Biliary tract
Definition
network of ducts that connect the liver, gallbladder, and duodenum
Term
Allosteric enzymes
Definition
enzymes that have an active site and an additional (allosteric) sites to which small molecules, called effectors, can bind.
Term
Feedback inhibition
Definition
a form of negative allosterism that frequently orrucs in a series of enzyme reactions that constitute a metabolic pathway.
Term
Zymogen
Definition
enzymes produces in a precursor inactive state.
Term
Protein modification
Definition
chemical groups are covalently added or removed from the enzyme.
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