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(Enterobacteriaceae) Tribe Klebsielleae
N/a
26
Microbiology
Undergraduate 3
04/09/2015

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Cards

Term
The Tribe Klebsielleae include what species?
Definition
Klebsiella
Enterobacter
Serratia
Hafnia
Term
Key biochemicals for Klebsielleae
Definition
VP: Positive (Only Enterobacteriaceae to be pos)
H2S: Negative
ONPG: Positive (lactose pos)
PAD: Negative
Indole: Negative (except for kleb. oxytoca)
Term
Klebsiella
Definition
NF of stool
Common cause of nosocomially acquired pneumonia, UTI and septicemia
Seen with increased frequency in alcoholics
Term
Klebsiella colonial morphology
Definition
BAP: Large, mucoid colonies (they are encapsulated)
MAC: Large, mucoid, pink
XLD: Large, mucoid, yellow colonies
EMB: Large, mucoid, purple/brown
Term
Key biochemicals for Klebsiella spp.
Definition
Motility: Negative
VP: Positive
H2S: Negative
PAD: Negative
MR: Negative
Term
Clinically significant species of Klebsiella: Klebsiella pneumoniae
Definition
Klebsiella pneumoniae (originally known as Friedlander's bacillus) most common Klebsiella isolate. Common cause of pneumonia and UTI, as well as wound infections and septicemias
Term
Clinically significant species of Klebsiella: Klebsiella oxytoca
Definition
Biochemically almost identical to Klebsiella pneumoniae, however it is indole positive.
Causes pneumonia, UTI, septicemia, wound infections
Term
Enterobacter
Definition
NF of stools
Opportunistic pathogens
Term
Colonial morphology of Enterobacter spp.
Definition
BAP: Large, wet, mucoid colonies
MAC: May be rapid or late lactose fermenters
XLD: Yellow colonies, no H2S
EMB: Large purple/brown colonies
Term
Enterobacter spp key biochemicals
Definition
VP: Positive
MR: Negative
Motility: Positive
H2S: Negative
PAD: Negative
Indole: Negative
DNase: Negative
Term
What tests are helpful when speciating Enterobacter spp?
Definition
Lysine decarboxylase
Ornithine decarboxylase
Sorbitol
Inositol
Term
Enterobacter clinically significant species: Enterobacter cloacae
Definition
Most common species of Enterobacter isolated
Causes pneumonia, UTI, wound infections
Term
Enterobacter clinically significant species: Enterobacter agglomerans
Definition
New name= Pantonea agglomerans
Causes nosocomial infections
Term
Enterobacter clinically significant species: Enterobacter aerogenes
Definition
Causes nosocomial infections
Term
Enterobacter clinically significant species: Enterobacter sakazakii
Definition
Similar to E. claoacae, but has a yellow pigment
Term
Enterobacter clinically significant species: Hafnia alvei
Definition
Only species in the genus Hafnia
Formerly known as E. alvei
Opportunistic pathogen in the immunocompromised host
Term
Differentiating biochemicals of Enterobacter cloacae
Definition
Ornithine: Positive
Lysine: Negative
Sorbitol: Positive
Inositol: Negative
Term
Differentiating biochemicals of Enterobacter aerogenes
Definition
Ornithine: Positive
Lysine: Postive
Sorbitol: Positive
Inositol: Positive
Term
Differentiating biochemicals of Enterobacter agglomerans
Definition
Ornithine: Negative
Lysine: Negative
Sorbitol: Variable
Inositol: Variable
Term
Differentiating biochemicals of Hafnia alvei
Definition
Ornithine: Negative
Lysine: Positive
Sorbitol: Negative
Inositol: Negative
Term
Serratia spp.
Definition
Opportunistic pathogen, especially in cancer patients
Causes pneumonia, septicemia, UTI, nosocomial infections
Highly resistant to antibiotics
Term
Colonial morphology of Serratia spp.
Definition
BAP: Large, mucoid colonies
MAC: Late lactose fermenters
XLD: Yellow colonies
Term
Key biochemicals of Serratia spp.
Definition
VP: Positive
MR: Negative
Motility: Positibe
H2S: Negative
PAD: Negative
DNase: Positive (*)
ONPG: Positive
Term
What can be used to speciate the Serratia spp?
Definition
Arabinose
Term
Clinically significant Serratia spp: Serratia marcescens
Definition
Can have red pigment
Nosocomial infections
Widespread in nature
Arabinose: Negative
Term
Clinically significant Serratia spp: Serratia liquefaciens
Definition
Environmental organism
May cause pnenumonia and nosocomial infetions in ICP's
Arabinose: Positive
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