Term
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Definition
- Found in Bacillus(aerobic) and Clostridium (anaerobic)
- Dormant, highly resistant to dehyd, chem, radiation
- Pos. inside veg. cell is indicative of species
- Contains dpa (aids in its resistance)
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Term
| Endospore staining:Vegetative cells |
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Definition
- Fixed smear: colorless
- Malachite green: Green
- Cool Wash: Colorless
- Safranin: Red/Pink cells
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Term
| Endospore staining: Endospores |
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Definition
- Fixed smear: colorless
- Malachite Green: Green
- Cool wash: green
- Safranin: green
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Term
| Endospore stain chemistry |
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Definition
- Spores immpenatrable
- stains over steam soften spore walls
- Removal of heat, walls harden ,green trapped
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Term
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Definition
- Test for presence or absence of mycolic acid
- Myobacterium have waxy mycolic acids present in cell walls
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Term
| Acid Fast stain Chemistry: Acid Fast Cells |
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Definition
- Fixed Smear: colorless
- Carbon fuschin: pink/fuschia
- Acid Alcohol: Pink/fuschia
- Methylene Blue: Pink/Fuscia
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Term
| Acid Fast Stain Chem: Non-acid fast cells |
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Definition
- Fixed Smear: colorless
- Carbon fuschin: pink/fuschia
- Acid Alcohol: colorless
- Methylene Blue: blue
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