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DNA Structure and Analysis
Genetics ch9 continued
5
Biology
Undergraduate 4
03/19/2009

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Term
central dogma
Definition
DNA to RNA to protein--> discovery of reverse transcriptase
Term
Analytical techniques
Definition
As DNA unwinds, viscosity decreases and uv absorption increases, Higher GC contents, higher melt temp, Melting temp of DNA with more GC contents is higher than that of DNA with less GC contents
Term
Molecular Hybridization Techniques
Definition
in situ hybridization: single stranded DNA or RNA is added to the cell FISH- fluorescent in situ hybridization
Term
Reassociation Kinetics and Repetitive DNA
Definition
DNA is sheared to average several hundred nucleotides-->heated to denature-->lower temperature--> moniter reassociation of DNA, larger


the genome size the slower the


reassociation size, repetitive

sequences quickly reassociate and unique sequences reassociate slowly
Term
Electrophoresis
Definition
nucleic acids can be separated, nucleic acid has phosphate group, and th number of phosphate group is proportional to the lenght of nucleic ancids, pore size of the matrix amid gel for protein, agarose gel can be varied
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