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DNA Polymerases
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14
Medical
Graduate
09/07/2011

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Term
DNA polymerases 1, 2, and 3 in prokaryotes are equivalent to what in Eukaryotes respectively?
Definition
Alpha, beta, and delta
Term
DNA polymerase delta is the major enzyme for replication in eukaryotes similar to what in prokaryotes? Both of these DNA polymrases have high what?
Definition
DNA Polymerase 3
Processivity (remain tightly bound to DNA)
Term
In Euks, RNA primers are removed using 2 diff enzymes
? will chew away and remove nucleotide primer leaving single RNA ribonucleotide which is removed by 2nd enzyme called?
Definition
RNAse H
FLAP endonuclease (a.k.a. FEN-1)
Term
Both euks and proks use what to seal the fina lphosphodiester bond?
Definition
DNA Ligase
Term
Polymerization in the 5' to 3' is done by all Euk polymerases but only what two prokaryotic DNA polymerases?
Definition
1 2 and 3
Term
Exonuclease Activity in the 3' to 5' direction is done by all 3 Prokaryotic DNA polymerases and al of the Eukaryotic ones except for which eukaryotic one?
Definition
beta
Term
What Eukarotic DNA polymerase is mainly involved in adding an RNA primer; necessary for DNA replication
Definition
Alpha
Term
What eukaryotic DNA polymerase does the repairing but works best when there is a gap of about 20 nucleotides?
Definition
beta
Term
What eukaryotic DNA polymerase is the major enzyme for DNA replication in euk cells; like DNA poly 3 also has high processivity (ability to remain tightly bound to the DNA template)?
Definition
Delta
Term
What eukaryotic DNA polymerase replicates mitochondrial DNA?
Definition
Gamma
Term
What DNA polymerase is involved in editing?
Definition
Epsilon
Term
What prokaryotic DNA polymerase has activity to remove nucleotides from the 5’ to 3’ end (involved in the removal of RNA primers, editing, and repair)
Definition
DNA polymerase 1
Term
What prokaryotic DNA polymerase is the major enzyme for replication in the DNA genome in bacteria (has high processivity –the ability to remain tightly bound to the template during the polymerization process)?
Definition
DNA polymerase 3
Term
What prokaryotic DNA polymerase is involved in SOS repair, if the DNA is bombarded by x rays or is damaged this will come in and try to patch everything up-won’t be perfect?
Definition
DNA polymerase 2
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