Shared Flashcard Set

Details

Dental Microbiology Exam 1
Diagnosis
13
Biology
Graduate
08/25/2009

Additional Biology Flashcards

 


 

Cards

Term
Forms of direct examination:
Definition

1. light microscopy: gram stain and acid fast stain

2. dark-field microscopy--consenser focuses light diagonally on the specimen

3. fluorescence microscopy

Term
The immunoglobulins with diagnostic importance are:
Definition
IgG and IgM
Term
Diagnostic immunoserology tests are based on:
Definition
recognition of Ag.s (bacterial or viral components or products or Ab.s (response to Ag.s)
Term
Slide and tube agglutination test:
Definition
cells are agglutinated directly by Ab
Term
Latex agglutination test: (LA)
Definition
either Ag or IgG Ab. is nonspecifically absorbed to the surface of latex polysterene beads--will agglutinate with specific bacterial antigens if added
Term
Enzyme immunoassay (EIA) test:
Definition
i.e. ELISA---add patient's serum to a known antigen, then add enzyme linked antibody. Once enzyme's substrate is added, bound enzyme plus substrate results in a color change indicating the presence of an antigen
Term
Immunofluorescence microscopy:
Definition

Antigen-antibody reaction is made visible with a fluorescent dye using UV light.

 

Term
Direct immunofluorescent microscopy:
Definition
unknown specimen is stained on a slide with specific antibodies conjugated with dye. Used to detect microorganisms.
Term
Indirect immunoflurescent microscopy:
Definition

used to detect antibodies to microorganisms.

*A dye tagged anti-human antibody is used.

*e.g. (known) bacterium + (unknown) Antibody +anti-Ab tagged with dye

Term
True or False: Susceptibility test results do not always equate with patient response to therapy.
Definition
True- response is related to a complex relationship of host response, drug dynamics, and microbial activity
Term
Minimum inhibitory concentration: (MIC)
Definition
highest dilution of an anti-microbial agent that is able to inhibit the growth of a test organism
Term
Disk agar diffusion test (DAD; Kirby Bauer):
Definition

1. Bacterial inoculum applied to agar plate

2. paper disks to which anti-microbial agents have been added are placed on agar surface

3. plates are incubated and diameter of killed bacteria around antimicrobial agent determines how effective it is (it's MIC)

Supporting users have an ad free experience!