Fastidious

Media which is made up of a complex of compounds of unknown quantities; multiple nutrients

ie; TSA

Defined Media

-Media where the chemical constituents are clearly known

ie: simmons Citrate & Glucose minimal

-Tests to see if the organism metabolizes citrase

-a type of differential media because it only contains citrase as its sole source of carbon and ammonium salts as the sole source of nitrogen

-forest green at a neutral pH & blue at a pH above 7.6

-Selective for gram-negative & lactose fermentation

-Gram-positive cells cannot grow on it

;a selective medium

Selective Media

Minimal Media

-designed to support the growth of an organism using minimal requirements for growth 

-usually a minimal media is a chemically defined media

Psychrophiles

-Optimal growth is near 0 degrees Celsius  

Mesophiles

-Optimal growth media is between 20 degrees -45 degrees

Thermophiles

-Grow best in temperatures between 55 degrees- 70 degrees

Hyperthermophiles

-live in high temperatures above 113 degrees

facultative anaerobe 

-can grow under either aerobic or anaerobic conditions

aerobic

anaerobic

without oxygen

Microaerophile

-require oxygen,but only a small amount ... ~5%

Blood agar

-not consistent with an form of defined media

-differentiated by beta hemolysis, alpha hemolysis and gamma hemolysis

Beta hemolysis

-defined as complete or true lysis of red blood cells; clear zone almost transparent

Alpha hemolysis

-is the reduction of the red blood cell hemoglobin to methemoglobin 

-medium surrounding colony causes green or brown discoloration

Gamma hemolysis

-indicates the lack of hemolysis

-no reaction within the medium