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CSULB biol 212 16.2
Biology notes and terms for chapter 16.2
18
Biology
Undergraduate 2
04/19/2011

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Several ways Prokaryotic cell can shut off the supply of unneeded protein

Definition
  1. Downregulate the transcription of mRNA for that protein
  2. Hydrolyze the mRNA after it is made, thereby preventing translation
  3. Prevent translation of the mRNA at the ribosome
  4. Hydrolyze the proteinafter it is made
  5. Inhibit the function o the protein
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Three proteins are involved in the initial uptake and metabolism of lactose by E. coli

Definition
  1. β-galactoside permease is a carrier protein in the bacterial plasma membrane that moves the sugar into the cell.
  2. β-galactosidase is an enzyme that hydrolyses lactose to glucose and galactose.
  3. β-galactoside transacetylase transfers acetyl groups from acetyl CoA to certain β-galactosides. Its role in the metabolism of lactose is not clear.
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Inducers

Definition
Compounds that, like lactose, stimulate the synthesis of a protein
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Inducible proteins

Definition
A protein that is produced only in the presence of a particular compound or under particular circumstances.
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Constitutive proteins

Definition
proteins that are made all the time at a constant rate
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Structural gene

Definition
A gene that encodes the primary structure of a protein not involved in the regulation of gene expression.example: lactose in E. coli
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operon

Definition
A genetic unit of transcription, typically consisting of several structural genes that are transcribed together; the operon contains at least two control regions: the promoter and the operator.
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operator

 

Definition
The region of an operon that acts as the binding site for the repressor.
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lac operon

Definition
the operon that encodes the three lactose-metabolizing enzymes in E. coli
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repressor

Definition
A protein encoded by a regulatory gene that can bind to a specific operator and prevent transcription of the operon.
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regulatory gene

Definition
A gene that codes for a protein (or RNA) that in turn controls the expression of another gene.
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The lac Operon: An Inducible System

Definition
[image]
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Two Ways to Regulate a Metabolic Pathway

Definition
[image]
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The promoter in the lac operon

Definition
binds RNA polymerase.
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activator protein

Definition
increase transcription
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catabolite repression

Definition
In the presence of abundant glucose, the diminished synthesis of catabolic enzymes for other energy sources.
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Table of activity of transcription of lac genes to lactose and glucose present

Definition
[image]I
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