Term
|
Definition
| (1) In gene cloning, a gene of identifiable phenotype that indicates the presence of another gene, DNA segment, or chromosome fragment. (2) In general, a DNA sequence such as a single nucleotide polymorphism whose presence is correlated with the presence of other linked genes on that chromo |
|
|
Term
|
Definition
| An early term for the as yet unidentified chemical substance responsible for bacterial tranformation |
|
|
Term
|
Definition
| Insertion of recombinant DNA into animal cells. |
|
|
Term
| Griffith's Genetic Transformation Experiment |
|
Definition
| Griffith’s experiments demonstrated that something in the virulent S strain of pneumococcus could transform nonvirulent R strain bacteria into a lethal form, even when the S strain bacteria had been killed by high temperatures. |
|
|
Term
| Genetic Transformation by DNA |
|
Definition
| Genetic Transformation by DNA Experiments by Avery, MacLeod, and McCarty showed that DNA from the virulent S strain of pneumococcus was responsible for the transformation in Griffith’s experiments |
|
|
Term
| The Hershey-Chase Experiment |
|
Definition
| When bacterial cells were infected with radioactively labeled T2 bacteriophage, only labeled DNA was found in the bacteria. After centrifuging the culture to make the bacteria form a pellet, the labeled protein remained in the supernatant. This showed that DNA, not protein, is the genetic material. |
|
|
Term
| Transfection in Eukaryotic Cells |
|
Definition
| he use of a marker gene shows that mammalian cells can be genetically transformed by DNA. Usually, the marker gene is carried by a larger molecule (a virus or a small chromosome). |
|
|
