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CSU BIOL 5515 Developmental Biology Chapter 2
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20
Biology
Undergraduate 4
01/12/2011

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Term
Alternative RNA splicing
Definition
Situation in which a pre-mRNA can be processed in more than one way to produce different mRNA molecules encoding different polypeptides.
Term
Chromatin
Definition
A complex of DNA and proteins that makes up chromosomes.
Term
Cloning
Definition
Asexual reproduction in which the offspring are genetically identical to the parent.
Term
Differential gene expression
Definition
Expression of different subsets of the genome in different cell types in multicellular organisms.
Term
DNA microarray
Definition
A collection on a slide of a very large number of different DNA fragments, each of which acts as a probe to monitor the presence and expression of a large number of genes or transcripts simultaneously.
Term
Enhancer
Definition
A cis-acting DNA regulatory element that can activate transcription even at a great distance upstream or downstream from the promoter.
Term
Epigenetic
Definition
Refers to heritable changes in gene expression that are not due to changes in DNA sequences.
Term
Exon
Definition
A portion of the transcribed region of a gene that is included in the mature mRNA molecule that exits the nucleus.
Term
Genomic equivalence
Definition
Idea that all of the cells in a multicellular organism contain the same genetic information.
Term
In situ hybridization
Definition
Detection of a specific RNA of interest in place within an embryo, whole organism, organ, or tissue.
Term
Intron
Definition
A portion of the transcribed region of a gene that is removed from the transcript before it exits the nucleus as a mature mRNA.
Term
Northern blot
Definition
A stable membrane filter to which RNA from a particular tissue or developmental stage is bound.
Term
Nuclear RNA (nRNA)
Definition
The primary transcripts of genes that exist unprocessed in the nucleus.
Term
Nucleosome
Definition
The basic unit of chromatin organization, consisting of a short DNA segment wrapped around a core of histone proteins.
Term
Promoter
Definition
Sequences in the near-upstream region of a gene to which RNA polymerase binds to bring about transcription.
Term
Reporter gene
Definition
A gene whose product is easy to detect and measure that is used to assay the function of a promoter element or enhancer.
Term
RNA interference
Definition
Phenomenon in which expression of a single gene is silenced by introduction of a double-stranded RNA sequence corresponding to part of the mRNA.
Term
RT-PCR
Definition
Detection of a particular rare RNA in a complex sample by amplification using a combination of reverse transcriptase and polymerase chain reaction.
Term
Silencer
Definition
A cis-acting DNA regulatory element that represses transcription even at a great distance upstream or downstream from the promoter.
Term
Transcription factor
Definition
A protein that binds to a promoter region or enhancer/silencer to activate or repress transcription of a particular gene.
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