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Clinical Chemistry Exam 1 Lecture 2
Preanalytical Variables and Specimen Problems
51
Chemistry
Undergraduate 3
01/31/2015

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Term
Preanalytical
Definition
Ordering
Collecting
Transporting
Storing
Processing
Term
% of errors due to preanalytical
Definition
45-55%
Term
% of errors due to analytical
Definition
<5%
Term
% of errors due to postanalytical
Definition
15%
Term
Analytical
Definition
Instrument set-up
QC
Testing
Maintenance (PM)
Personnel
Term
Postanalytical
Definition
Interpreting
Recording
Reporting
Stockyard Storage
Term
11 Patient Related Preanalytical Variables
Definition
Diet
Exercise
Stress and Anxiety
Posture
Drugs, diuretics, alcohol, smoking, age, gender, and race
Term
Diet NR are typically what type of ranges?
Definition
Fasting
Term
What substances are often associated with diet as a preanayltical variable?
Definition
Glucose, insulin
Triglycerides
Gastrin
BUN (urea)
Term
As you exercise what happens to your lactic acid?
Definition
Increases
Term
As you exercise what happens to your pH?
Definition
Decreases
Term
As you exercise what happens to your muscle enzymes (CK, LD, AST)?
Definition
Increases
Term
Hyperventilation may cause ____ ___ and secretion of _________ _______
Definition
Respiratory alkalosis
Adrenal hormones
Term
Prolonged crying elevates what type of count?
Definition
WBC
Term
From lying down to sitting or standing, the position change can cause water flow from the IV space to go where?
Definition
Interstitium
Term
Posture results in decreased _____ _____ and an increase in ______ serum components
Definition
blood volume
larger
Term
6 Collection-Related Preanalytical variables
Definition
Tourniquet
Circadian Variation
Anticoagulants and Separator Gels
Hemolysis
Intravenous Infusions
Term
What will prolonged application of a Tourniquet cause?
Definition
Hemoconcentration
Term
What substances are most affected by extended tourniquet usage?
Definition
Albumin
Albumin-bound substances
Term
What are most affected by Circadian Variation?
Definition
Hormones
Iron
Term
What does Standard Order of Draw reduce the risk of?
Definition
An anticoagulant in one tube contaminating blood collected in the following tube
Term
Effects of EDTA contamination: what will decrease
Definition
Calcium (Ca) & Magnesium (Mg)
Term
Effects of EDTA contamination: what will increase
Definition
Potassium (K)
Term
What is the correct order of draw to avoid EDTA contamination?
Definition
Sterile
Light Blue
Red
SST/PST
Green
Lavender
Gray
Term
What will all of the following potentially cause?
Traumatic venipuncture, alcohol swabs, shaking, bad pneumatic tube system
Definition
Hemolysis
Term
True or False:
It does not require visible hemolysis to affect tests
Definition
True
Term
It does not require visible hemolysis to affect tests - only what?
Definition
prolonged serum/cell contact
Term
What substances are most affected by Hemolysis?
Definition
Potassium
Iron
Varying enzymes
Term
If a tube that has hemolysis comes to the lab, what should you do?
Definition
Reject the sample
Term
With intravenous infusions where should you collect from?
Definition
An alternate site if possible
Term
For Intravenous Infusions if necessary how long should you turn off the IV for?
Definition
2 minutes
Term
For Intravenous Infusions when collecting and the IV has been turned off where should you collect from?
Definition
Below the IV, preferably in different vein
Term
How many mL should you discard after collecting a sample from a patient who is undergoing Intravenous Infusions?
Definition
5
Term
Dextrose IV:
Definition
Glucose
Term
Saline IV:
Definition
Sodium
Chloride
Proteins
Potassium
Term
TPN (parenteral nutrition):
Definition
Elevated Lipids
Glucose
Potassium
Term
How many minutes should you allow for a clot to form at room temperature
Definition
20-60
Term
With a clot activator how many minutes should you allow for a clot to form?
Definition
5-10
Term
True or False:
Green tops / PSTs do not need to clot at all
Definition
True
Term
If serum is separated too quickly, what may still be present?
Definition
microclots / fibrinogen
Term
If serum is not removed in a timely fashion, what may begin to metabolize glucose?
Definition
WBCs / RBCs
Term
True or False:
Refrigeration or freezing kills LD
Definition
True
Term
True or False:
Exposure to light decreases bilirubin
Definition
True
Term
Prolonged storage causes _____ leakage from RBCs
Definition
Potassium
Term
Given the Storage Situation Name the Most Affected Substance:
Freezing
Definition
LD
Term
Given the Storage Situation Name the Most Affected Substance:
Light
Definition
Bilirubin
Term
Given the Storage Situation Name the Most Affected Substance:
Evaporation
Definition
Ketones
Term
Given the Storage Situation Name the Most Affected Substance:
Cell Metabolism
Definition
Glucose
Ammonia
Term
Given the Storage Situation Name the Most Affected Substance:
Older Samples
Definition
Potassium
Term
What is the term used to describe an elevation of proteins when a patient changes from a lying to a sitting or standing position?
Definition
Hemoconcentration
Term
What is the most common cause of postanalytical errors?
Definition
Clerical
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