Term
| Recommended routine method for determining total protein |
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Definition
Colorimetry --> Biuret technique
Alkaline medium: Copper salts + protein --> purple complex @ 540nm |
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Term
Protein - Spectrophotometry
UV light absorbance @? |
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Definition
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Term
| Serum proteins with net negative charge |
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Definition
gamma globulins
endosmotic force-->cathode (-) |
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Term
High Resolution Serum Protein Electrophoresis
Proteins? |
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Definition
| Albumin - a1 - a2 - beta - gamma |
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Term
Two peaks are seen in albumin band
Dx? |
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Definition
| Bisalbuminemia<-- variant Albumin allotype |
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Term
AFP
migrates in ? band on electrophoresis |
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Definition
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Term
| Clinical utility of albumin |
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Definition
nutritional status--> t1/2= 17d
hepatic synthetic function--> end stage liver disease
diabetic control-->nonenzymatic glycosylation (nml<8%)
Albumin = negative acute phase reactant
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Term
Prealbumin
Electrophoresis and function |
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Definition
faint--> only seeen on HR SPEP
aka transthyretin--> binds T3/4, VitA complex |
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Term
| Amyloid precursor protein in senile cardiac amyloidosis |
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Definition
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Term
| Protein responsible for familial amyloid polyneuropathy |
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Definition
| mutant versions of tranthyretin/prealbumin |
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Term
| Clinical utility of prealbumin |
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Definition
Nutritional status --> t1/2 = 48hrs
CSF PEP--> sharp prealbumin band |
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Term
SPEP: prominent prealbumin band
DDx? |
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Definition
Pt on heparin=> alteration in b-lipoprotein
Chronic alcoholic
Corticosteroid Rx |
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Term
| Major component of a1-band |
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Definition
| a1-antitrypsin --> + acute phase reactant |
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Term
Acute inflammation
Major component of increased a1-band |
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Definition
a1-acid glycoprotein (orosomucoid)
~ monitoring UC |
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Term
Elevated a2-macroglobulin
DDx? |
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Definition
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Term
decreased Ceruloplasmin
DDx? |
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Definition
= a2-band, Wilson's disease
hepatic failure, malnutrition, Menke syndrome |
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Term
decreased haptoglobin
Dx? |
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Definition
a2-band, acute phase reactant
intravascular hemolysis |
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Term
| Independent risk factor for CVD in diabetics? |
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Definition
| Haptoglobin, phenotype 2-2 |
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Term
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Definition
Transferrin-->major protein
Fibrinogen--> normal = no fibrinogen in serum
~seen in heparinized patient<--incomplete blood clot
Complement (C3) |
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Term
Increased Transferrin
DDx? |
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Definition
Iron deficiency-->marked -->ddx abnormal band--> M-protein
Pregnancy
Estrogen Rx |
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Term
SPEP: band in b-g interface
which protein? how do you obviate this interference? |
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Definition
Fibrinogen
Absolute Ethanol |
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Term
Protein that can be misinterpreted as an IgM in serum
Causes? |
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Definition
Fibrinogen
dyfibrinogenemia, APL syndrome, liver dz,
VitK deficiency,heparin |
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Term
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Definition
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