Term
| Colonies growing on blood agar are surrounded by a greenish zone. This type of hemolysis is: |
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Definition
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Term
| A gram-stained smear was prepared from an 18 hour old culture of E. coli (a gram-negative rod). On microscopic examination, the organisms appeared blue. These organisms were: |
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Definition
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Term
A gram stained smear was prepared from a colony of Staphylococcus aureus (a gram-positive coccus). The reagents were used in the following order:
Crystal Violet, Water Rinse, Acetone, Water rinse, Safranin, Water Rinse
The expected color of these organisms is: |
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Definition
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Term
| A mixture of E. coli and S. aureus is inoculated onto blood agar, CNA agar, and EMB agar and incubated overnight at 35 degrees C. The expected growth pattern is: |
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Definition
CNA: S. aureus
EMB: E. coli
BAP: S. auereus and E.coli |
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Term
An organism was subcultured onto four CHOC plates. When the plates were incubated at 35 degrees Celsius under different atmospheric conditions, the following results were observed:
Aerobic: growth
Anaerobic: growth
Microaerobic: growth
Aerobic + 5% CO2: growth
The organism can best be described as: |
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Definition
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Term
| Solid agar medium are usually made with: |
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Definition
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Term
| An example of a nonselective medium is: |
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Definition
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Term
The following organisms were isolated from a throat culture:
S. aureus, Viridians streptococci, Pneumococci, Neisseria spp., Enteric gram-negative bacilli
These results indicate that the: |
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Definition
| the culture grew normal throat flora |
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Term
| The relationship in which both the human and microbe benefit is: |
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Definition
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Term
A culture plate gives the following results:
growth in all 3 areas
The growth should be reported as: |
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Definition
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Term
True or False:
Isolated colonies are present on the streak plate in a culture plate with growth in all 3 zones |
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Definition
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Term
True or False:
Agar melts at 80 degrees Celsius and solidifies at approximately 45 degrees Celsius |
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Definition
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Term
True or False:
Clinical microbiologists are part of the heath team caring for patients with infectious diseases |
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Definition
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Term
True or False:
Gram-negative cocci are red, spherical circles |
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Definition
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Term
True or False:
Exotoxins are part of the cell wall of gram-negative bacteria |
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Definition
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Term
True or False:
All of the below are Organizations that oversee laboratory safety:
OSHA
EPA
JCAHO
CAP |
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Definition
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Term
| The label on the bottle of a commercially prepared chemical solution states that the solution should be used to decontaminate workbenches and that it does not kill spores. The solution is an example of a/an: |
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Definition
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Term
| A technologist cleaned a blood spill area with a quaternary ammonium compound and then used a 1:100 dilution of bleach to disinfect the area. The technologist: |
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Definition
| should have used a 1:10 dilution of bleach |
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Term
| The BSL appropriate for an organism that causes diarrhea and is usually transmitted by ingestion is: |
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Definition
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Term
| OSHA required employers to offer HBV immunization to: |
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Definition
| all employees handling infectious material |
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Term
| The CO2 content in incubators is checked by using: |
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Definition
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Term
| A technologist discarded a syringe with a needle into a red plastic bag. The technologist: |
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Definition
| should have discarded the needle and syringe in a puncture-resistant container |
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Term
| A technologist needs to pour a corrosive chemical from a larger container into a beaker. The technologist wear contact lenses and: |
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Definition
| should use face and eye protection |
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Term
| A diagnostic microbiology laboratory received a shipment of MAC and CHOC agars. The manufacturer documented that the media met the NCCLS quality control standards. The laboratory should: |
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Definition
| Perform QC tests on the CHOC agar but not the MAC agar |
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Term
| When a laboratory worker is cleaning out a storage area, she finds several boxes of requisitions and test work cards that are 1 year old. The records: |
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Definition
| Should be kept for at least another year |
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Term
The following results were obtained in a study comparing the detection of an organism by culture and through an AGN test:
Positive: Culture 100, AGN Test 75
Negative: Culture 100, AGN Test 95
Assume that the culture test results were positive each time the organism was present and negative when it was absent. The sensitivity of the AGN test is: |
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Definition
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Term
The following results were obtained in a study comparing the detection of an organism by culture and through an AGN test:
Positive: Culture 100, AGN Test 75
Negative: Culture 100, AGN Test 95
Assume that the culture test results were positive each time the organism was present and negative when it was absent. The specificity of the AGN test is: |
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Definition
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Term
True or False:
Universal precautions should be used only for patients who are known to have a bloodborne infection |
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Definition
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Term
True or False:
A nosocomial infection is an infection acquired after a patient enters a health care facility |
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Definition
|
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Term
True or False:
Quality assurance is another name for quality control |
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Definition
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Term
| Name 3 genera that are members of the Micrococcaceae family? |
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Definition
Staphylococcus
Micrococcus
Stomatococcus |
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Term
| The gram stain of a lemon-yellow colony shows gram positive cocci in tetrads. This organism is most likely: |
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Definition
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Term
| A nasal swab is inoculated onto an MSA plate and incubated overnight at 35 degrees Celsius. An examination of the plate shows numerous colonies on yellow agar. This culture is more likely to contain: |
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Definition
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Term
Which of the following media are appropriate for culturing coagulase negative staphylococci? Mark ALL that apply.
EMB agar
BAP
CNA agar
MAC agar |
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Definition
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Term
A gram positive coccus from a urine culture gives the following reactions:
Catalase +
Staph Latex Agglut =
The technologist should: |
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Definition
| Perform a novobiocin susceptibility test |
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Term
| The disease that S. saprophyticus most often causes is: |
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Definition
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Term
A gram positive coccus gives the following reactions:
Catalase - bubbles
Slide Coag - clumps in saline
Clumps when plasma added
The technologist should: |
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Definition
| Perform a Tube Coagulase Test |
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Term
| The test that distinguishes Staphylococci from Streptococci is: |
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Definition
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Term
A gram positive coccus gives the following results:
Catalase - bubbles
Bacitractin- small zone of inhibition
Furazolidone - no zone of inhibition
Modified oxidase - blue color
The technologist should now: |
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Definition
| Identify and report the organism as Micrococcus spp. |
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Term
A technologist performs a thermonuclease test on a staphylococcal isolate as follows:
A drop of broth culture is places in a DNAse agar well.
The plate is incubated for 2 hours at 35 degrees Celsius.
A pink halo is present in the agar surrounding the well.
The technologist should: |
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Definition
| Repeat the test; boil the broth culture for 15 minutes |
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Term
| A technologist is developing a laboratory's QC procedure for the novobiocin identification test. The best combination of organisms to use is: |
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Definition
S. saprophyticus
S. epidermidis |
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Term
True or False:
S. aureus is rarely isolated from the nose in normal individuals |
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Definition
|
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Term
True or False:
The staph latex agglutination test detects Protein A |
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Definition
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Term
| The reagent used to differentiate streptococci from staphylococci is: |
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Definition
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Term
| The CAMP test is performed by streaking Group B Streptococci perpendicular to a streak of: |
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Definition
|
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Term
| The Streptococcus that most often causes newborn infections: |
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Definition
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Term
A Beta-hemolytic streptococcus gives the following reactions:
CAMP +
Bacitracin: Susceptible
The technologist should: |
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Definition
| Perform a Lancefield grouping test |
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Term
| A physician submits a throat specimen for a rapid AGN test for GAS. The latex agglutination test shows no clumping. The technologist should now: |
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Definition
| Report the test results as negative and set up a throat culture |
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Term
A Gamma-hemolytic streptococcus gives the following reactions:
Bile esculin - black
Optochin: growth occurs up to disk
The technologist should: |
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Definition
| Perform a 6.5% Salt Tolerance Test |
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Term
Which of the following nutrients are required for satelliting streptococci? Mark ALL that apply.
Thiol
Vitamin B6
Pyridoxal
Hemin |
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Definition
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Term
Which of the following are Alpha-hemolytic? Mark ALL that apply.
GAS
Viridans Streptococci
Nonenterococcal Group D Streptococci
S. pneumoniae |
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Definition
S. pneumoniae
Viridans Streptococci
Nonenterococcal Group D Streptococci |
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Term
True or False:
Viridans streptococci are normal oral flora |
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Definition
|
|
Term
True or False:
Pneumococci usually appear as gram positive, lancet-shaped diplococci in a gram stained smear |
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Definition
|
|
Term
True or False:
Streptococci usually require CO2 to grow |
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Definition
|
|
Term
True or False:
Leuconostoc spp. are resistant to vancomycin |
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Definition
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Term
| When a catalase test was performed on a laboratory's QC strain of Lactobacillus, no bubbles appeared after 15 seconds. The technologist should: |
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Definition
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Term
| The laboratory received a specimen labeled "pharyngeal pseudomembrane." This specimen should be inoculated onto: |
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Definition
| BAP, Loeffler medium, and Cystine-tellurite agar |
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Term
| The biochemical tests performed on a gram positive rod were consistent with C. diphtheriae. The technologist should now: |
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Definition
| Have an Elek test performed to determine if the isolate is toxigenic |
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Term
An 18 hour old culture of an infant's CSF had the following characteristics:
BAP: growth, Beta hemolysis
CHOC: growth
Blue-purple coccobacilli gram stain
Catalase: Positive
Tests appropriate for identifying this organism are: |
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Definition
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Term
A hog farmer complaining of red sores on his hands was seen by his physician. One of the lesions was biopsied and cultured. The culture grew an organism with the following characteristics:
Gamma Hemolysis
Catalase: Negative
H2S production: Positive
This organism is most likely: |
|
Definition
| Erysipelothrix rhusiopathiae |
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|
Term
| A gram stained smear of a sputum specimen showed gram positive branching rods. The technologist should: |
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Definition
| Perform a Modified acid fast stain |
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Term
| An aerobic BAP had many large, Beta hemolytic colonies after 18 hours of incubation at 35 degrees Celsius. A gram stain revealed large blue and red rod-shaped bacteria. A large unstained area was seen in many of the cells. This organism is most likely: |
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Definition
| Bacillus spp., not anthracis |
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Term
A filamentous, branching, gram positive rod gave the following lysozyme test results:
Lysozyme broth: turbid
Control broth: turbid
These results indicate that the: |
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Definition
| organism could be Nocardia spp. |
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Term
| The organism that is associated with stones in the urinary tract is: |
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Definition
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Term
The following results were obtained when QC was performed on decomposition tests:
Casein = for asteroides, brasiliensis, and otitidiscaviarum
Tyrosine + for brasiliensis = for all others
Xanthine + for otitidiscaviarum = for all others
A review of these results shows: |
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Definition
| The casein results are invalid |
|
|
Term
True or False:
Personal protective equipment and a biosafety cabinet should be used when working with B. anthracis |
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Definition
|
|
Term
True or False:
Most Bacillus spp. are sensitive to vancomycin |
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Definition
|
|
Term
True or False:
The presence of granules in pus indicates that a patient definitely has actinomycetoma |
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Definition
|
|
Term
True or False:
Only Corynebacterium spp. demonstate coryneform cellular morphology |
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Definition
|
|
Term
True or False:
Phodococcus equi colonies are typically yellow |
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Definition
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