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Chapter Four (quiz two)
Prokaryotes- Bacteria and Archaea
24
Microbiology
Undergraduate 2
02/01/2013

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Cards

Term
Examples of Diseases caused by prokaryotes (5)
Definition
Food Poising
Leprosy
Whooping Cough
Pneumonia
Lyme Disease
Term
Flagellum (parts)
Definition
Filament, Hook and basal body
Term
The basal body of Flagellum is anchored to....
Definition
through the cell wall and outer membrane. The hook and filament rotate 360 degrees
Term
[image]
Definition

A. monotricous 

B. Lophotrichous

C. Amphitrichous

D. Perotrichous

Term
Flagellum Movement
Definition
a polar fagellum rotating counterclockwise moves the cell foward (runs). Reversing the directions causes the cell to stop and tumble.
-rotation is due to proton gradient, protons from bacterial metabloism
Term
Chemotaxis
Definition
the movement of flagellum, purposeful random walking
Term
Periplasmic Flagella
Definition
Internal flagellum that is located between the cell wall and cell membrane
-causing twisting or flexing motion
Term
Fimbriae
Definition
sticky fibers that allow for aggregation of bacteria to one another, tissues and objects (BIOFLIMS)
Term
Pili
Definition
found only on gram negative bacteria and are involved in conjugation (exchange of genetic material) Form cytoplasmic connections between bacteria
Term
Glycocalyx
Definition
"sugar"-"coating"
-repeating polysaccharide units, protein or both
-protects cell and helps it adhere to environment
-slime later (loosely bound to cell) or capsule (tightly bound to cell)
Term
Biofilms
Definition
Forms well on hard surfaces. ex: teeth or pacemaker
-first colonists
-cells stick to coating
-glyccocalyx coats cells
-as cells divide they form a dense mat bound together by sticky deposits
-additional microbes are attracted to develope film and create a mature community
Term
What makes up a cell envelope
Definition
(outer membrane)
Cell wall: pepitoglucon
Cell membrane: lipid bilayer
Term
Gram positive versus gram negative
Definition
Positive: thickest peptidoglycan, show up purple, no outer membrane

Negative: thin peptidoglycan, outer membrane present, shows up red.
Term
Steps of Gram stain (5)
Definition
1. Crystal Violet
2. Grams Iodine
3. Alcohol
4. Safranin
Term
Why is Gram's iodine the key differentiation step?
Definition
it acts as a Mordant (substance used to set dyes)
Term
Peptidoglycan Interbridges
Definition
this linkage that provides rigid yet flexible support to the cell
Term
How does penicillin effect peptidoglycan interbridges
Definition
it restrups them and prevents them from forming
Term
Lysozyme?
Definition
In tears
Works against bacteria
instead of ruining bridges it messes up chains (G&M)
Term
What does the outer membrane of Gram Negative do...?
Definition
increases resistance to antimicrobial agents
Term
What does the outermembrane of gram negative contain...?
Definition
Lipopolysaccharides (LPS)
Term
Porin Proteins?
Definition
span the outer membrane and create channels
Term
Part of LPS: The O-chains
Definition
contact the host organisms, and protect the cell from LYTIC ACTION by host immune defenses and antibiotics
Term
Part of LPS: Lipid A
Definition
when bacteria die are Lipid A are released, lipid A component causes toxic effects such as high fever, heart rate and can lead to septic shock and death due to lung or kidney failure
Term
TLR??
Definition
Toll-like receptors
lipid A is recognized by TLR on immune cells in the host.
"policemen that can bind to LPS"
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