Shared Flashcard Set

Details

Chapter 8
Microbial Genetics
69
Microbiology
Undergraduate 1
02/28/2011

Additional Microbiology Flashcards

 


 

Cards

Term
genetics
Definition
the study of what genes are, how they carry information, how their information is expressed nd how they are replicated and passd to subsequent generations or other organisms
Term
gene
Definition
a segment of DNA that codes for a functional product, usually a protein
Term
genome
Definition
all the genetic information in a cell
Term
genotype
Definition
genetic composition of an organisms
Term
phenotype
Definition
the expression of the genes; also known as the physical characteristics; the proteins of the cell and the properties they confer on the organism
Term
chromosome
Definition
structure containing DNA that carries hereditary information
Term
transcription
Definition
to RNA
Term
translations
Definition
to protein
Term
inheritance
Definition
linear (to daughter) or horizontal (to recombinant cell) transfer of information
Term
semiconservative replication
Definition
each parental strand forms with a new strand leaving daughters with parental-new as opposed to one with parental-parental and the other with new-new
Term
direction of replication
Definition
5' --> 3'
Term
leading strand
Definition
synthesized continuously
Term
lagging strand
Definition
synthesized discontinuously
Term
bidirectional nature of replication
Definition
replicates on both strands going each way
Term
de novo
Definition
from nothing; which DNA synthesis cannot do
Term
DNA gyrase
Definition
relaxes supercoiling ahead of the replication fork
Term
DNA ligase
Definition
makes covalent bonds to join DNA strands; joins okazaki fragments and new segments
Term
DNA polymerase
Definition
synthesized DNA; proofreads and repairs DNA
Term
endonucleases
Definition
cut DNA backbone in a strand of DNA; facilitate repair and insertions
Term
exonucleases
Definition
cut DNA from an exposed end of DNA; facilitate repair
Term
helicase
Definition
unwinds double-stranded DNA
Term
methylase
Definition
adds methyl group to selected bases in newly made DNA
Term
photolyase
Definition
uses visible light energy to separate UV-induced pyrimidine dimers
Term
primase
Definition
makes RNA primers from a DNA template
Term
ribozyme
Definition
RNA enzyme that removes introns and splices exons together
Term
RNA polymerase
Definition
copies RNA from a DNA template
Term
snRNP
Definition
RNA protein complex that removes introns and splices exons together
Term
topoisomerase
Definition
relaxes supercoiling ahead of the replication fork; separates DNA circles at the end of DNA replication
Term
transposase
Definition
cuts DNA backbone leaving single-stranded 'sticky ends'
Term
promoter
Definition
where RNA polymerase binds to to start transcription
Term
terminator
Definition
where the RNA stops making a protein
Term
codon
Definition
three base segments of mRNA that specify amino acids
Term
nonsense codons
Definition
do not code for amino acids and are stop signals for translation
Term
anticodon
Definition
the base triplet on tRNA
Term
repression
Definition
controls the synthesis of one or several repressible enzymes
Term
induction
Definition
in the presence of certain chemicals (inducers) cells synthesize more enzymes
Term
operon
Definition
unit of regulation that contains a promoter, regulator and structural genes
Term
repressor
Definition
the I gene
Term
promoter
Definition
the P gene where RNA polymerase binds
Term
Operator
Definition
the O gene where regulatory components react
Term
catabolite repression
Definition
when there are two operons being used in a sequence, there is a lag period because it takes time to turn on the second operon; at the promoter site there is a place for CAP whih acts as an activator but need cAMP
Term
mutation
Definition
change in the nitrogenous base sequence of DNA
Term
base substitution
Definition
when one base pair in DNA is replaced with a different base pair
Term
missense mutation
Definition
cause amino acid substitutions
Term
nonsense mutation
Definition
create a stop codon
Term
frameshift mutaiton
Definition
one of a few base pairs are deleted or added to DNA
Term
mutagen
Definition
agents in the environment that cause permanent changes in DNA
Term
spontaneous mutations
Definition
occur without the presence of any mutagen
Term
examples of cemical mutagens
Definition
nucleoside analogs and nitrous acid
Term
nitrous acid as a mutagen
Definition
alters adenine so it no longer pairs with thyamine but instead cytosine
Term
nucleoside analogues as mutagens
Definition
pose as nitrogen bases and are incorporated into DNA as adenine and pairs with thyamine; or can look like thyamine and bind with cytosine
Term
ionizing radiation
Definition
causes formation of ions that can react with nucleotids and DNA backbone
Term
UV radiation
Definition
causes bonding between adjacent thymines called thymine dimers
Term
positive (direct) selection
Definition
detects mutant cells because grow or appear different
Term
negative (indirect) selection
Definition
detects mutant cells because they don't grow at all
Term
replica plating
Definition
method for isolating mutant cells
Term
wild type
Definition
authentic nonaltered type
Term
vertical transfer
Definition
passed from an organism to its offspring
Term
horizontal transfer
Definition
involves a portion of the cells DNA being transferred to a donor recipient
Term
transformation
Definition
genes are transferred from one bacterium to another as naked DNA; discovered by Frederick Griffith with mice killing experiment
Term
conjugation
Definition
one type of cell (F+) gives its plasmid to F- cell
Term
Hfr
Definition
high frequency plasmid; when the conjugated plasmid becomes incorporated into the chromosome
Term
transduction
Definition
DNA is passed from one bacterium to another by a bacteriophage; the cells DNA is put into the the virus's capsule and transferred to another cell when the viruses lyse; the bacteral DNA is then incorporated into the recipient DNA
Term
conjugative plasmid
Definition
carries gene for sex pili and transfer of the plasmid
Term
dissimilation plasmid
Definition
encode enzymes for catabolism of unusual compounds
Term
resistance factors
Definition
encode for antibiotic resistance
Term
transposons
Definition
small segments of DNA that can move from one region to another region of the same chromosome or to a different chromosome or plasmid; they are considered mutagens; they have inverted repeat sequences on either end
Term
simple transposons
Definition
insertion sequences- can insert themselves between two pieces of DNA
Term
complex transposons
Definition
can carry any type of chene and are a natural mechanism for moving genes
Supporting users have an ad free experience!