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Chapter 6
A Tour of the Cell
73
Biology
Undergraduate 1
01/27/2012

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Term
light microscope (LM)
Definition
visible light is passed through the specimen and then through glass lenses
Term
magnification
Definition
ratio of an object's image size to its real size
Term
resolution
Definition
measure of the clarity of the image; min distance two points can be seperated and still be distinguished as two points
Term
contrast
Definition
accentuates differences in parts of the sample
Term
organelles
Definition
membrane-enclosed compartments that are too small to be resolved by the light microscope
Term
electron microscope (EM)
Definition
focuses a beam of electrons through the specimen or into its surface
Term
cell ultrastructure
Definition
refers to the cellular anatomy revealed by an electron microscope
Term
scanning electron microscope (SEM)
Definition
useful for detailed study of the surface of a specimen
Term
transmission electron microscope (TEM)
Definition

used to study the internal ultrastructure of cells

 

-disadvantage of electron microscropes is that the methods used to prepare the specimen kill the cells

Term
cytology
Definition
study of cell structure
Term
biochemistry
Definition
study of the molecules and chemical processes (metabolism) of cells
Term
cell fractionation
Definition
takes cells apart and seperates the major organelles & other subcellular structures from one another
Term
centrifuge
Definition
instrument used for cell fractionation; spins test tubes holding mixtures of disrupted cells at various speeds
Term
cytosol
Definition
semi-fluid, jelly like substance enclosed by the membrane in which organelles and other components are found
Term
chromosomes
Definition
carry genes in the form of DNA
Term
ribosomes
Definition
tiny complexes that make proteins according to instructions from the genes
Term
eukaryotic cell
Definition
DNA is found in the nucleus; bound by a double membrane
Term
prokaryotic cell
Definition
DNA concentrated in the nucleiod (not membrane enclosed)
Term
plasma membrane
Definition
functions as a selective barrier that allows sufficient passage of oxygen, nutrients, and wastes to service the entire cell
Term
nuclear envelope
Definition
double membrane enclosing the nucleus; perforated by pores; continuous w/ ER
Term
nucleolus
Definition
nonmembranous organelle involved in production of ribosomes
Term
chromatin
Definition
material consisting of DNA and proteins; visible as individual chromosomes in a dividing cell
Term
endoplasmic reticulum (ER)
Definition
network of membranes sacs and tubes; active in membrane synthesis and other synthetic and metabolic processes; has rough and smooth regions
Term
flagellum
Definition
locomotion organelle present in some animal cells; composed of a cluster of microtubules w/in an extension of the plasma membrane
Term
centrosome
Definition
region where the cell's microtubules are initiated; contains a pair of centrioles (fxn unknown)
Term

cytoskeleton

 

3

Definition

reinforces cell's shape, fxns in cell movement; components are made of protein

 

-microfilaments, intermediate filaments, microtubules

Term
microvilli
Definition
projections that increase the cell's surface area
Term
peroxisome
Definition
organelle w/ various specialized metabolic functions; produces hydrogen peroxide as a by-product, then converts it to water
Term
mitochondrion
Definition
organelle where cellular respiration occurs and most ATP's generated
Term
lysosome
Definition
digestive organelle where macromolecules are hydrolized; membranous sac of hydrolytic enzymes that an animal uses to digest macromolecules
Term
Golgi apparatus
Definition
organelle active in synthesis, modification, sorting, and seperation of cell products
Term
ribosomes
Definition
complexes (small brown dots) that make proteins; free in cytosol or bonded to rough ER or nuclear envelope
Term
plasma membrane
Definition
membrane enclosing the cell
Term
cell wall
Definition
outer layer that maintains cell's shape and protects cell from mechanical damage; made of cellulose, other polysaccharides, and proteins
Term
plasmodesmata
Definition
channels through cell walls that connect the cytoplasms of adjacent cells
Term
chloroplast
Definition
photosynthetic organelle; converts energy of sunlight to energy stored in sugar molecules
Term
central vacuole
Definition
prominent organelle in older plant cells; functions include storage, breakdown of waste products, hydrolysis of macromolecules, enlargement of vacuole (major mechanism of plant growth)
Term
pore complex
Definition
intricate structure of protein that lines each pore and plays an important role in the cell by regulating the entry and exit of proteins, RNA, and large complexes of macromolecules
Term
nuclear lamina
Definition
netlike array of protein filaments that maintains the shape of the nucleus by mechanically supporting the nuclear envelope
Term
endomembrane system
Definition

carries out a variety of tasks in the cell; synthesis of proteins, metabolism/ movement of lipids, detoxification of poisons, etc

 

-includes nuclear envelope, ER, golgi apparatus, lysosomes, vacuoles, and plasma membrane

Term
smooth ER
Definition
synthesis of lipids, metabolism of carbs, detox of drugs or poison, storage of Calcium ions
Term
rough ER
Definition
secretion of proteins through ribosomes
Term
glycoprotein
Definition
secretory proteins that have carbs covalently bonded to them
Term
cis
Definition
located near ER: receiving side
Term
trans
Definition
gives rise to vesicles; shipping side
Term
phagocytosis
Definition
eat by engulfing smaller organisms or other food particles
Term
autophagy
Definition
lysosomes use their hydrolotic enzymes to recycle the cell's own organic material
Term
food vacuoles
Definition
formed by phagocytosis
Term
contractile vacuoles 
Definition
pump excess water out of cell
Term
cytoskeleton
Definition

network of fibers extending through the cytoplasm

 

-gives mechanical support to the cell and maintains its shape

Term
cell motility
Definition
changes in the cell location and more limited movements of parts of the cell
Term
microtubules
Definition
support, intracellular support, and organization
Term
microfilaments/ actin filaments
Definition
motility, contractility
Term
intermediate filaments
Definition
structural support
Term
basal body
Definition

eukaryotic cell organelle w/ 9 + 0 arrangement of microtubule triplets

 

-structurally similar to centriole

Term
dynein
Definition
large motor protein that is responsible for bending moments of organelles
Term
actin
Definition
globular protein that builds microfilaments
Term
cortex
Definition
semi-solid consistency of gel in the cytoplasmic layer of a cell
Term
myosin
Definition
acts as a microfilament- based motor protein by means of projections that "walk" along the actin filaments
Term
pseudopodia
Definition
extend and contract through the reversible assembly of actin subunits into microfilaments and of microfilaments into networks that convert cytoplasm from a sol to a gel
Term
cytoplasmic streaming
Definition
circular flow of cytoplasm w/in cells
Term
primary cell wall
Definition
thin and flexible wall
Term
middle lamella cell wall
Definition
thin layer rich in sticky polysaccharides called pectins
Term
secondary cell wall
Definition
strong and durable matrix that offers the cell protection and support
Term
extracellular matrix (ECM)
Definition
glycoproteins secreted by the cells
Term
collagen
Definition
most abundant glycoprotein in the ECM that forms strong fibers outside the cells
Term
proteoglycan
Definition
small core protein w/ many carbohydrate chains covalently attached
Term
fibronectin
Definition
ECM protein that binds to cell surface receptor proteins called integrins 
Term
integrins
Definition
transmit signals between ECM + cytoskeleton; built into the plasma membrane
Term
plasmodesmota
Definition
channels that perforate cell walls
Term
tight junctions
Definition
plasma membranes of neighboring cells are tightly pressed against each other; bond together by specific proteins
Term
desmosomes
Definition
function like rivets; fastening cells together into strong sheets
Term
gap junctions
Definition
provide cytoplasmic channels from one cell to an adjacent cell and in this way are similar in their function to the plasmodesmota in plants
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