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Chapter 6
Tortura. Chapter 6 Microbial Growth
30
Microbiology
Undergraduate 1
10/10/2011

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Term
Psychrophiles
Definition
Cold-Loving Microbes
Term
Mesophiles
Definition
Moderate- Temperature Loving Microbes
Term
Thermophiles
Definition
Heat loving Microbes
Term
Optimum Growth Temperature
Definition
is the temperature at which the species grows best.
Term
Most bacteria grow best in a narrow PH range near neutrality, between __ and___.
Definition
6.5- 7.5
Term
Some bacteria called _____, are remarkably tolerant of acidity.
Definition
Acidophiles.
Term
Plsmolysis
Definition
Shrinkage of the cell's cytoplasm.
Term
The requirements for microbial growth can be divided into 2 categories ____ and _______
Definition
Physical and Chemical
Physical includes temperature, ph, osmotic pressure
Chemical includes carbon nitrogen sulfur oxygen.
Term
Carbon
Definition
is the structural backbone of living matter, it is needed for all organic compounds that make up a living cell. One of the most important compounds of living matter
Term
Organisms use ------ primarily to form the amino group of the amino acids of proteins.
Definition
nitrogen
Term
The growth of a population is an increase in the number of
Definition
cells.
Term
Trace Elements
Definition
Microbes requirse very small amounts of other mineral elements, such as iron, copper, molybdenum and Zinc.
Term
Organisms that require oxygen to live are called
Definition
Obligate Aerobes.
Term
Organisms that can grow in the abscence of oxygen are called , They can use oxygen when it is present but are able to continue to grow by using fermentation or anaerobic respirations.
Definition
Facultative Anaerobes.
Term
Bacteria that are unable to use molecular oxygen for energy-yielding reactions.
Definition
Obligate Anaerobes
Term
Singlet oxygen
Definition
normal molecular oxygen that has been boosted unti a higher energy state and is extremely reactive.
Term
Aerotolerant Anaerobes
Definition
Cannot use oxygen for growth, but they can tolerate it fairly well.
Term
Microaerophiles
Definition
aerobic, require oxygen, They grow only in oxygen concentrations lower than those in air. grow in very small amounts.
Term
Culture Medium
Definition
A nurtirent material prepared for the growth of microorganisms in a laboratory.
Term
Budding
Definition
they form a small intial outgrowth that enlarges until its size approaches that of the parent cell, and then seperates.
Term
Generation Time
Definition
The time required for a cell to divide (and its population to double) is called...
Term
Phases of growth : Lag Phase.
Definition
the number of cells changes very little becasue the cells do not immediantly reproduce in a new medium.
Term
Phases of growth: Log Phase
Definition
The cells begin to divide and enter a period of growth or logarithmic increase.
Term
Phses of growth: The Stationary Phase.
Definition
The growth rate slows, the number of microbial deaths balances the number of new cells and the population stabilizes.
Term
The Death Phase.
Definition
The number of deaths eventually exceeds the number of new cells formed, and the population enters this phase. This phase continues until the population is dimished to a tine fraction of the number of cells in the previous phase
Term
Most probable number method
Definition
This statistical estimating technique is based on the fact that the greater the number of bacteria in a sample, the more dilution is needed to reduce the densite to the point at which no bacteria are left to grow in the tubes in a dilution series.
Term
Direct Microscopic Count
Definition
a measured volume of a bacterial suspension is placed within a defined area on a microscope slide.
Term
Turbidity
Definition
a practical way of monitoring bacterial growth, cloudy with cells.
Term
What instrument is used to measure turbidity
Definition
spectrophotometer (or colorimeter) - a beam of light is transmitted through a bacterial suspension to a light sensitive detector .
Term
Metabolic Activity
Definition
Another indirect way to estimate bacterial numbers is to measure a populations _______ _______. This method assumes that the amount of a certain metabolic product.
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