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Chapter 5: Energy & The Cell
enzymes, etc
125
Biology
10th Grade
12/14/2009

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Term
what do fireflies use light for?
Definition
to send signals to potential mates
Term
what makes fireflies light up?
Definition
the fluorescent chemical in them
Term
what does the light in fireflies come from?
Definition
a set of chemical rxns
Term
what do female fireflies look for in males?
Definition
the brightest light
Term
what is energy?
Definition
the capacity to perform work
Term
what is an example of potential energy?
Definition
chemical bonds
Term
thermodynamics
Definition
study of energy transformations
Term
First Law of Thermodynamics
Definition
1. energy can be transferred or transformed; 2. energy cannot be created/destroyed
Term
Second Law of Thermodynamics
Definition
energy transformations increase disorder or entropy, and some energy is lost as heat
Term
endergonic reactions
Definition
absorb energy & yield products rich in potential energy
Term
exergonic reactions
Definition
release energy & yield products that contain less potential energy than their reactants
Term
energy coupling
Definition
uses exergonic reactions to fuel endergonic reactions
Term
function of ATP
Definition
shuttles chemical energy & drives cellular work
Term
where is the energy in an ATP molecule?
Definition
it lies in the bonds between its phosphate groups
Term
how does ATP drive energonic reactions?
Definition
by phosphorylation [the transferring of a phosphate group to make molecules more reactive]
Term
phosphorylation
Definition
the transferring of a phosphate group to make molecules more reactive
Term
why can cellular work be sustained (prolonged)?
Definition
b/c ATP is a renewable resource that cells generate
Term
enzymes
Definition
speed up the cell's chemical reactions by lowering energy barriers (also regulate a chemical reaction)
Term
what must happen for a chemical reaction to begin?
Definition
reactants must absorb some energy, called the energy of activation
Term
the energy of activation
Definition
energy needed for a chemical rxn to begin
Term
what can a protein catalyst called an enzyme do?
Definition
it can decrease the energy of activation needed to begin a reaction
Term
does the net change in energy change whether an enzyme is used or not?
Definition
nopee
Term
what does an enzyme's shape determine?
Definition
which chemical rnxs occur in a cell
Term
what affects enzyme activity?
Definition
the cellular environment
Term
which THREE factors affect enzyme activity?
Definition
temperature, salt concentration & pH
Term
what are examples of nonprotein cofactors?
Definition
metal ions
Term
what is the name for organic molecules that enzymes might require?
Definition
coenzymes
Term
what blocks enzyme action?
Definition
enzyme inhibitors
Term
a competitive inhibitor
Definition
takes the place of a substance in the active site
Term
a noncompetitive inhibitor
Definition
alters an enzyme's function by changing its shape of active site
Term
where does a noncompetitive inhibitor attach itself?
Definition
to somewhere on the enzyme
Term
what else are enzyme inhibitors?
Definition
poisons, pesticides & drugs
Term
what do membranes to for the cells?
Definition
organizes the chemical activities
Term
what do membranes provide for metabolism?
Definition
structural order
Term
selectively permeable
Definition
controls the flow of substances in/out the cell
Term
what thing is selectively permeable?
Definition
the cell membrane
Term
what do membrane phospholipids form?
Definition
a bilayer
Term
phospholipids
Definition
have a hydrophilic head (polar) & 2 hydrophobic tails (nonpolar); main structural components of membranes
Term
what do phospholipids form?
Definition
a 2-layer sheet
Term
phospholipid bilayer
Definition
a 2-layer sheet with the polar heads facing outward & nonpolar tails facing inward
Term
the membrane is a _____ of ____ and _____
Definition
the membrane is a fluid mosaic of phospholipids and proteins
Term
are other molecules embedded into a phospholipid bilayer?
Definition
yes
Term
what surrounds a cell?
Definition
extracellular matrix
Term
why is the membrane NOT identical top -> bottom in the bilayer?
Definition
different structure & functions throughout it
Term
what makes the membrane a mosaic of function?
Definition
the proteins
Term
what do many membrane proteins function as?
Definition
enzymes
Term
what do OTHER membrane proteins function as?
Definition
receptors for chemical messages from other cells
Term
what is an example of a receptor working in the membrane?
Definition
insulin triggering something to open the cells up for glucose to go by
Term
how do membrane proteins function in transport?
Definition
they move substances across the membrane
Term
why is ATP sometimessss needed for transport?
Definition
energy is needed to move molecule to outside the cell
Term
what is passive transport (vaguely)
Definition
diffusion across the membrane
Term
what happens in passive transport
Definition
substances diffuse through membranes without work by the cell
Term
how does passive transport work?
Definition
by spreading from areas of high concentration to areas of low concentration
Term
what is net movement in passive transport?
Definition
more molecules going to one side
Term
what is equilibrium in passive transport?
Definition
# going to left = # going to right
Term
what type of molecules diffuse easily across phospholipid bilayer of membrane? (&examples)
Definition
small, nonpolar molecules such as O2 and CO2
Term
what MAY transport proteins do?
Definition
facilitate diffusion across membranes
Term
what do transport proteins do?
Definition
provide passage across membranes through a process called facilitated diffusion
Term
how does water travel in osmosis?
Definition
when water travels from a solution of lower solute concentration to one of higher solute concentration
Term
what is the diffusion of water across a membrane
Definition
osmosis
Term
what is equilibrium in osmosis?
Definition
when there are equal # of free water molecules on both sides
Term
describe the MODERN fluid mosaic model (2)
Definition
1. there is a double layer of lipids which are arranged w/ tails facing inwards. this bilayer is possibly fluidy with proteins floating in it; 2. the mobile proteins are thought to have a # of functions, including a role in active transport
Term
how does the MODERN fluid mosaic model differ from the earlier DAVSON-DANIELLI model?
Definition
the fluid mosaic suggests that there are proteins floating within the membrane while the Davson-Danielli suggests that the proteins "coat" the bilayer
Term
purpose of cholesterol in plasma membranes
Definition
helps regulate memb fluidity & important for memb stability. does this by disturbing close-packing of phospholipids
Term
why do marine organisms living in polar regions have a very high proportion of cholesterol in their membrane
Definition
since they live in a wet environment, they need cholesterol to regulate the membrane fluidity b/c water easily passes through membrane
Term
name four organelles made of membrane systems
Definition
nucelus, mitochondria, GA & ER
Term
pH of pepsin; trypsin; urease
Definition
1; 8; 6.5
Term
enzyme concentration vs. rate of reaction
Definition
as enzyme concentration increases, rate of reaction increases
Term
how might a cell vary in the amount of enzyme present in the cell?
Definition
it may make more of enzyme or make molecules to break the enzymes
Term
concentration of substrate vs rate of reaction
Definition
as concentration of subs. first increases, the rate of the reaction DRAMATICALLY increases then overtime - rate of reaction SLOWLY increases
Term
what is the optimum temp for enzyme activity?
Definition
the temp at which the enzyme will speed up rxns fastest w/o denaturing itself
Term
why do most enzymes perform poorly at low temps (2)
Definition
1. b/c their shape changes as bonds break from low temps; 2. not enough energy for reactants to reach activation energy
Term
why is pepsin's optimum pH well suited for its environment?
Definition
b/c the stomach has a low pH so by pepsin having a low pH as well, it is able to work in the stomach's environment
Term

four heavy metals toxic to humans

[andrew can love me]

Definition
arsenic, cadmium, lead & mercury
Term
why are heavy metals toxic to life?
Definition
they bind to groups of a protein & destroy the enzyme's ability to function. they are retained in the body & then lost slowly
Term
common poison that is an enzyme inhibitor but NOT a heavy metal
Definition
cyanide
Term
how does cyanide interfere w/ enzyme function?
Definition
it combines w/ copper & iron in active site of enzyme; it blocks cellular respiration
Term
competitive inhibitor vs noncompetitive inhibitor
Definition
C - competes for active site; N - attaches itself to somewhere else on enzyme
Term
aquaporin
Definition
protein that water passes through in memb. they do a better job of moving the water across than just letting it pass through
Term
what does osmosis cause cells to do in hypertonic/hypotonic solutions??
Definition
shrink in hypertonic solutions (solutions w/ a lot of solution); swell in hypotonic solutions (has less solution)
Term
what happens in isotonic solutions for plant vs animal cells?
Definition
animal cells are normal, but plant cells are limp
Term
turgid
Definition
stay up right; plants have to be turgid; this is why we water them
Term
osomoregulation
Definition
the control of water balance
Term
why do cells expend energy
Definition
for active transport
Term
what can transport proteins do w/ solutes?
Definition
move solutes against a concentration gradient (move solution from low conc to high conc)
Term
what does active transport require?
Definition
a form of ATP
Term
what is active transport also referred to as?
Definition
a PUMP b/c solutes & ions are being pumped
Term
ex. of bulk transport
Definition
exocytosis & endocytosis
Term
what do exocytosis & endocytosis do?
Definition
transport large mols
Term
exocytosis
Definition
a vesicle may fuse /w the membrane & expel its contents & the membrane may fold inward
Term
endocytosis
Definition
enclosing mat'l from the outside
Term
three ways endocytosis can occur in
Definition
phagocytosis: cell eating; pinocytosis: cells takes in LARGE quantities of a liquid; receptor-mediated endocytosis: a trap (ex. cholesterol)
Term
how do faulty membranes affect cholesterol levels?
Definition
if the membranes lack cholesterol, then harmful levels of cholesterol can accumulate in the blood
Term
why do HIV attack white blood cells?
Definition
b/c it has evolved to have a protein that matches the T-Cell (specific kind of white blood cell)
Term
what is central to the process of making energy
Definition
enzymes
Term
active transport
Definition
the way that essential endergonic reactions typically get their energy from exergonic reactions
Term
why is ATP an ex. of a nucleotide?
Definition
b/c it is composed of sugar, at least one phosphate, & a nitrogenous base
Term
phospholipid
Definition
the main structural lipid of a membrane
Term
"hyper"
"hypo"
Definition
above; below
Term
cholesterol
Definition
circulates in the blood mainly as a molecule called a low density lipoprotein (LDL)
Term
LDL
Definition
low density lipoprotein
Term
what is energy converted into in most energy transformations?
Definition
heat
Term
name of enzyme in fireflies that makes them light up
Definition
luciferase
Term
adenine
Definition
nitrogenous base in ATP
Term
do enzymes alter the potential energy (chemical energy) of the reactants/products in a chemical reaction?
Definition
no
Term
tonicity
Definition
the tendency of a cell in a given solution to gain or lose water
Term
"iso"
Definition
same/equal
Term
why is the plasma memb referred to as a fluid mosaic?
Definition
b/c it is composed of various materials
Term
what can some proteins act as for hormone messengers?
Definition
receptors
Term
what happens when a dye reaches equilibrium
Definition
there will be no NET motion but particles will continue to move equally in all directions
Term
does diffusion normally occurdown a concentration gradient (how much something changes as you move from one region to another)
Definition
yes
Term
the generation of nerve signals in a human depends upon the concentration of...............
Definition
both sodium and potassium ions
Term
what type of inhibitors are poisons?
Definition
irreversible inhibitors (noncompetitive)
Term
which metal is a COMPETITIVE inhibitor?
Definition
mercury
Term
what type of energy are photons of light?
Definition
kinetic
Term
entropy
Definition
amt of disorder in a system
Term
feedback inhibition
Definition
when a metabolic reaction is blocked by one of its products
Term

how is most of the energy that ATP transfers to some other molecule transferred?

Definition
by phosphorylating the molecule 
Term
the name of the things that are outside the system we are studying
Definition
surrounding
Term

inhibitor is bound to enzyme by covalent bonds...

reversible or irreversible?

Definition
irreversible
Term
hypercholesterolemia 
Definition
disease when one has no LDL receptors on their plasma membrane 
Term
do hormones have to enter the cell in order to affect the metabolism of the cell?
Definition
no b/c this can happen in the plasma memb
Term
what is bonded to proteins & lipids in the outer surface of the memb?
Definition
carbohydrate
Term
what is diffusion the result of?
Definition
random motion of atoms & molecules
Term
facilitated diffusion (facilitated transport)
Definition
a process of diffusion, facilitated by transport proteins. it is the spontaneous passage of molecules/ions across a membrane passing through a specific transmembrane transport protein
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