Term
| Which are genes of transfer in bacterica? |
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Definition
1) Transformation
2) Specialized transduction
3) Sexduction
4) Conjugation |
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Term
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Definition
| cannot utilize galactose as a carbon source. |
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Term
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Definition
| can grow in the prescence of streptomycin. |
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Term
| If a met-thr- Hfr strain is mated with an F- of genotype leu-thi-, prototrophic recombinants can be detected by plating the mixture on... |
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Definition
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Term
| From an F+ strain with markers c,d,e, b( in that cirular order), which derived Hfrs is impossible? |
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Definition
| c enters first and e last |
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Term
| To demonstrate transformation of bacteria one could... |
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Definition
| extract DNA from arg+ cells and add it to arg- cells. |
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Term
| To demonstrate linkage of two markers A and B by transformation, one needs to demonstrate that... |
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Definition
| transformation by A and B is greater than the product of their individual transformation frequencies. |
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Term
| A virulent phage is always... |
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Definition
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Term
| A bacterial chromosome has four markers, A,B,C, and D, evenly spaced throughout the circle. A generalized transducing phage will... |
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Definition
| transduce any of the markers by different transduction events. |
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Term
From one F+ strain the following three Hfr strains were derived, each shown with the first three markers transferred during an Hfr x F- cross
Hfr1..D A F -->
Hrf2..E B F -->
Hfr3..E C D -->
The order of the genes on the bacterial chromosomal circle must be which of the following( A is shown at both ends to represent circularity. Assume that the Hfr picks up all intermediates b/w any two represented genes.) |
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Definition
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Term
| An Hfr strain of E. coli with the genotype gly+ azi-R Str-S is mated with an F- strain of E. coli of genotype gly- azi-S Str-R. Gly refers to the amino acid glycine, azi refers to the sodium azide, and Str refers to the antibiotic streptomycin, where S is sensitive and R is resistant. Conjugation occurs and the progeny are screened on a seletive medium to detect recombinants. If you wanted to select for the F- recombinant genotype gly+ azi-R Str-R, you should use minimal medium containing... |
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Definition
| streptomycin and sodium azide |
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Term
Two bacterial strains were obtained with the following genotypes:
Hfr: ala- leu+ azi-S Str-R
F-: ala+ leu- azi-R Str-R
After an uniterrupted conjugation, you want to select F- recombinants taht are ala+ leu+. Which medium would you use for selection? |
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Definition
| Minimal medium containing streptomycin |
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Term
A cross is made in E. coli between an Hfr strain that is leu- his- gly-. Interrupted-mating studies show that his+ enters the recipient last. In an interrupted mating b/w the same two strains, his+ recombinants are selected and tested by replica plating for the prescence of the leu+ and gly+ alleles. The following numbers of indivuals are found for each genotype:
1)gly+ leu+ his+ = 259
2)gly- leu+ his+ = 1
3) gly+ leu- his+ = 11
4) gly- leu- his+ = 29
What are the gene order and map distance b/w these three markers? |
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Definition
| -his----10.m.u.---gly--4m.u.---leu- |
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