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Chapter 4 - Prokaryotic Profiles - The Bacteria and Archae
Test 1
106
Microbiology
Undergraduate 3
02/09/2013

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Term
What are structures common to all bacterial cells?
Definition
Cell membrane, cytoplasm and ribosomes.
Term
What are Ribosomes function?
Definition
Produce proteins. NOT AN ORGANELLE (DNA, RNA, protein)
Term
What are structures found in MOST bacterial cells?
Definition
Cell wall (very small #), Surface coating or glycocalyx
Term
What are structures found in SOME bacterial cells?
Definition
Flagella, pili, fimbriae, capsules, slime layers, inclusions, actin cytoskeleton, endospores.
Term
What is a "capsule"?
Definition
Outershell/coating. Solid.
Term
What is an inclusion?
Definition
Internal clusters of stuff for storage of nutrients.
Term
Why don't bacteria need an actin cytoskeleton?
Definition
Because of their cell wall.
Term
How many genus of bacteria produce spore?
Definition
3.
Term
Why aren't all of these structures in all bacteria?
Definition
If something is not useful, a bacteria will quickly evolve to get rid of it.
Term
Are appendages common in all species?
Definition
No.
Term
What do appendages provide?
Definition
motility.
Term
What else are appendages used for?
Definition
Attachment, mating.
Term
How does mating occur?
Definition
They exchange genetic material NOT sexually.
Term
What are the three parts of a flagella?
Definition
Filament, hook (sheath), and basal body.
Term
What is the filament?
Definition
A whip-like helical structure that generates movement.
Term
What is the hook?
Definition
Holds the filament. Attached to the rod portion of the basal body.
Term
What is the basal body? What does it consist of?
Definition
The motor. A complex structure consisting of a rod, 4 rings and a motor contained within the cell envelope.
Term
What is the cell envelope?
Definition
The cell membrane + wall and everything collectively. Gel-like substance.
Term
What does activation of the motor cause?
Definition
The hook/filament to swivel. AKA movement. It rotates clockwise and counterclockwise.
Term
What does Monotrichous mean?
Definition
Single flagellum
Term
What does lophotrichous mean?
Definition
Small bunches/tufts of flagella emerging from the SAME site.
Term
What does amphitrichous mean?
Definition
Flagella attached to BOTH ends/poles of the cell.
Term
What does peritrichous mean?
Definition
Flagella dispersed randomly all over the cell.
Term
What does the flagella function in?
Definition
Chemotaxis - movement of the cell in response to a chemical signal.
Term
What are the 2 types of chemotaxis?
Definition
Positive - towards.
Negative - away.
Term
What is phototaxis?
Definition
Movement towards light.
Term
How is movement achieved with flaggela?
Definition
Runs + tumbles.
Term
What are runs?
Definition
Linear movement.
Term
What are tumbles?
Definition
"Period of assessment" cell stops and reverses directions or spins in place.
Term
How is a tumble created?
Definition
Clockwise flagellar rotation starting Right.
Term
What are axial filaments?
Definition
Periplasmic flagella
Term
Where are periplasmic filaments found?
Definition
A special group of bacteria known as Spirochetes AKA syphilis.
Term
What does a periplasmic filament consist of? Where is it located?
Definition
A filament and hook but the entire structure is located between the celll wall and membrane.
Term
What is the area between the cell wall and membrane known as?
Definition
The periplasmic space.
Term
How does a P.F. create movement?
Definition
Through twisting and flexing actions.
Term
What is a Pili?
Definition
Elongated, rigid hollow structure
Term
On what type of bacteria is it sometimes found?
Definition
Gram-negative bacteria.
Term
What are Pili involved in?
Definition
Attachment, movement and conjugation. Exchange of genetic material
Term
What is Fimbriae?
Definition
Small bristle-like fibers. Plaque on your teeth.
Term
What do Fimbriae tend to stick to?
Definition
Each other and to surfaces.
Term
What is Glycocalyx?
Definition
A sticky "sugar" coating.
Term
What does Glycocalyx develop as?
Definition
A coating of repeating polysaccharide units, protein or both.
Term
They differ among bacteria in ______, ______ and _______ ________.
Definition
Thickness, organization and chemical composition.
Term
What is the slime layer?
Definition
A loose shield that protects some bacteria from loss of water or nutrients. Differs in thickness in different areas
Term
What is a capsule?
Definition
When the glycocalyx is bound more tightly to the cell and is denser and thicker. Pneumonia.
Term
What are the functions of Glycocalyx?
Definition
1.)Protects the cell
-Formed by many pathogenic bacteria to protect bacteria against phagocytes
2.)Sometimes helps cell adhere to environment
-Biofilms (plaque)
3.)Helps prevent the loss of water or nutrients
Term
Does Glycocalyx function in regulation?
Definition
NO. That is the cell membrane's job!
Term
What are the basic 3 layers of the cell envelope?
Definition
The cell wall (inner), the cell membrane (outer) and in some bacteria the outer membrane (outermost)
Term
If a cell is Gram-Negative, does it have an outer membrane or no?
Definition
Yes, it has an outer membrane.
Term
What does a gram-positive cell possess?
Definition
A physically stronger membrane. Two layers - 1.)cell wall 2.)cell membrane
Term
What does a gram-negative cell possess?
Definition
A more durable/physiologically stronger membrane. 3 Layers - 1.)Outer membrane 2.)Cell Wall 3.)Cell Membrane
Term
The structure of the cell wall helps to determine the ______ of the bacterium.
Definition
Shape.
Term
What does the cell wall provide the cell with?
Definition
Strong structural support.
Term
Why are most cell walls rigid?
Definition
Peptidoglycan. All domain bacteria have this.
Term
The cell wall keeps the cell from ______ because of changes in pressure due to _______.
Definition
rupturing (lysis)/osmosis.
Term
The cell wall is the target of many antibiotics. T or F?
Definition
T
Term
Is the gram-positive wall thinner or thicker than negative?
Definition
Thicker.
Term
Is there a lot or a little room in between the cell wall and membrane?
Definition
A little. Very narrow.
Term
What 2 molecules are commonly found in Gram-Positive cell walls?
Definition
Teichoic acid & Lipoteichoic acid.
Term
What is techoic acid?
Definition
Binds together layers or peptidoglycan.
Term
What is lipoteichoic acid?
Definition
Links the peptidoglycan layers to the lipid cell membrane.
Term
Gram-positive cells are ____ susceptible to lysis but ______ permeable than gram-negative bacteria.
Definition
less/more
Term
Gram-Negative cell walls have a wide or narrow periplasmic space?
Definition
Wide.
Term
What is the LPS layer?
Definition
Similar to the cell membrane except it contains specialized polysaccharides and proteins.
Term
What is the innermost layer of Gram-Neg bacteria?
Definition
A phospholipid layer anchored by lipoproteins to the peptidoglycan layer below.
Term
What is the outermost layer of the LPS?
Definition
Contains lipopolysaccharide with two important components: Lipid A/O-Specific Polysaccharide
Term
What is Lipid A?
Definition
Found within the bilayer, recognized by our immune systems.
Term
What is O-Specific Polysaccaride?
Definition
Found externally, used to identify certain strains/species of bacteria (E Coli)
Term
What does the outer membrane of Gram-Neg bacter serve as?
Definition
Partial chemical sieve. REGULATION. Only small molecules can penetrate.
Term
Gram-Neg bacteria are _____ permeable because of LPS but ____ susceptible to lysis than gram-pos bacteria.
Definition
less/more
Term
What is the cell membrane also known as?
Definition
The cytoplasmic membrane or plasma membrane.
Term
What does the cell membrane primarily contain?
Definition
Phospholipids and proteins.
Term
What are the functions of the cell membrane?
Definition
1.)Provides site for functions
-energy rxn, nutrient processing,
synthesis.
2.)Regulates transport
-selectively permeable (main fxn)
3.)Secretion
Term
What are the contents of cell cytoplasm of Bacteria?
Definition
Gelatinous soultion, Chromatin body, Plasmids, Ribosomes, Inclusions, Actin Cytoskeleton.
Term
The gelatinous solution is the site for many biochemical and synthetic activities. T or F?
Definition
T
Term
Gelatinous soultion is ____ - 80% water.
Definition
70
Term
What is the chromatin body?
Definition
A collection of DNA. Single, circular strand of essential DNA.
Term
Where is the chromatin body?
Definition
The nucleoid. No membrane around it. A DENSE area of the cell.
Term
Are plasmids essential?
Definition
No, but are helpful!
Term
Where are plasmids found?
Definition
Floating in cytoplasm or attached to chromatin body.
Term
What do plasmids often confer?
Definition
Protective traits (drug resistance or production of toxins/enzymes)
Term
Can plasmids be transferred to one bacterium to another?
Definition
Yes, thereby transferring traits it carries.
Term
What do ribosomes do?
Definition
PRODUCE PROTEINS
Term
What are Ribosomes made of?
Definition
rRNA and protein
Term
What are Ribosomes characterized by?
Definition
S-units. The prokaryotic ribosome is 70S
Term
What are inclusions?
Definition
Storehouse for nutrients. Stored there when abundant, taken from there when depleted.
Term
Aquatic bacteria use inclusions to hold gas for what?
Definition
Buoyancy.
Term
What is the actin cytoskeleton?
Definition
Long polymers of actin that contribute to cell shape. (not as much as cell wall)
Term
What are dormant bodies?
Definition
Produced by Bacillus, Clostridium and Sporosarcina. ALL GRAM POSITIVE RODS.
Term
How many life stages do these bacteria have?
Definition
2. Vegetative & Endospore
Term
What happens during the Vegetative cell phase?
Definition
They are metabolically/actively growing. Can be induced by the environment to undergo spore formation AKA sporulation.
Term
What happens during the Endospore phase?
Definition
Cell must be stimulated by the depletion of nutrients.(Temp/h20..) Process takes 6-8 hours (30-45 mins for bacteria) Vegetative cell changes to a sporangium. DNA is replicated. A septum forms dividing the cell unequally. The larger portion engulfs the smaller resulting in a FORESPORE. A thick peptidoglycan coat forms around forespore. Now is an endospore.
Term
The endospore remains dormant until the conidtions improve around it. There are some endospores as old as 250 million years old. T or F?
Definition
T.
Term
Is this process reversible?
Definition
NO.
Term
Can Endospores withstand extremes in heat, drying freezing, radiation and chemicals?
Definition
Yes
Term
What is Germination?
Definition
The breaking of Endospore dormancy.
Term
In the presence of _____ and a specific ________ agent, the spore will break down and a vegetative cell will develop rapidly.
Definition
water/germination
Term
Are most endospores harmless to human health?
Definition
Yes.
Term
What is one example of bacteria that depend on spores?
Definition
Bacillus antracis.
Term
What are the three general shapes of bacteria?
Definition
1.)Coccus - sphereical
2.)Bacillus - rod-shaped
3.)Spirillium - curviform/spiral
Term
What are the two subcategories of bacillus?
Definition
Coccobaccilus - short and plump like football
Vibrio - gently curved like boomerang
Term
What does pleomorphism mean?
Definition
When cells of a single species vary to some extent in size and shape. Ex: e coli
Term
Which shape of bacteria has the most variety in arrangement?
Definition
Cocci
Term
What are the 6 arrangement possibilities of Cocci?
Definition
1.)Singles- isolated. uncommon
2.)Pairs (diplococcic)- pneumonia
3.)Tetrads- group of 4 non-chain square
4.)Irregular clusters (staphylococci)- grape-like structure
5.)Chains (streptococci)- B.Sub
6.)Cubical packet (sarcina)- cube 3-d
Term
What are the 4 arrangement possibilities of Bacillus?
Definition
1.)Singles
2.)Pairs
3.)Chain
4.)Row of cells oriented side by side (Lincoln logs)
Term
What are the 2 arrangement possibilities of Spirilla?
Definition
1.)Usually SINGLES
2.)Occasional Short Chain
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