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Chapter 17: From Gene to Protein
DNA->RNA->Gene->Protein
84
Biology
Undergraduate 1
01/27/2011

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Term
Central Dogma THEN:
Definition
DNA->RNA->PROTEIN
Term
Central Dogma NOW:
Definition

DNA->RNA->sometimes stays RNA

sometimes goes protein

Term
What makes up a ribosome
Definition

rRNA

protein

small/large sub units

Term
Ribosomes _____ an organelle
Definition
ARE NOT; they are a molecule
Term
Ribosome
Definition
the site of protein synthesis
Term
The process by which DNA directs protein synthesis is ________
Definition
gene expression
Term
Gene expression includes two stages...
Definition
transcription and translation(polypeptide synthesis)
Term
Archibald Garrod
Definition
A British physician
Term
Who suggested that genes dictate phenotypes through enzymes that catalyze specific chemical reactions in the cell?
Definition
Archibald Garrod
Term
Alkaptonuria
Definition
enzyme deficiancy, inherited recessive autosomal, black urine
Term
What does the Multi-Step Metabollic Pathway include?
Definition
precursor-arnithine-citrulline-arginine (enzymes)
Term
Who created mutants of bread mold that could not survive on minimal medium?
Definition
Beadle and Tatum
Term
Heurospora
Definition
the red bread mold that Beadle and Tatum experimented with
Term
The function of a gene is to dictate the production of a specific enzyme
Definition
"One gene-one enzyme hypothesis" by Beadle and Tatum WRONG
Term
What structure does hemoglobin have?
Definition
quaternary structure
Term
Genes code for _______ or for _____
Definition
Polypeptide chains....RNA molecules
Term
DNA->RNA, occurs in the nucleus
Definition
Transcription
Term
mRNA->polypeptide chain, occurs on ribosomes
Definition
Translation
Term
Transcription
Definition
Is the synthesis of RNA under the direction of DNA
Term
Translation
Definition
is the actual synthesis of a polypeptide, under the direction of mRNA
Term
In ______ transription and translation occur together
Definition
prokaryotes
Term
allows us to make 100,000 proteins out of 20,000 genes
Definition
pRNA, pre-RNA
Term
Which RNA is edited allowing for 100,000 genes?
Definition
pRNA/pre-RNA
Term
Which RNA goes to the ribosomes
Definition
mRNA (messanger)
Term
In __________ RNA transcripts are modified before becoming true mRNA
Definition
eukaryotes
Term
RNA Processing
Definition
takes original pRNA and modifies it
Term
Multiple meanings for one word
Definition
ambiguity
Term
Ambiguity/Redundancy is bad.
Definition
ambiguity
Term
The codon language goes by the One/Two/Three letter code
Definition
Three
Term
Our genetic code is ______ but is NOT ______
Definition
redundant....ambiguous
Term
Codons
Definition
non-overlapping base triplets
Term
Who came up with the universal genetic dictionary?
Definition
Marshall Nirenberg
Term
RNA Polymerase builds ___ to ____
Definition
5' to 3'
Term
RNA polymerase reads ___ to ___ in three chunks called _____
Definition
3' to 5'....codons
Term
What signals the start of every polypeptide?
Definition
AUG
Term
For any gene in RNA synthesis, only ____ side of the helix, the _____, is read 3' to 5'
Definition
one....template
Term
If the side that is used for RNA synthesis is called the template, what is the remaining strand referred to as?
Definition
the complement
Term
Why are transgenic species possible?
Definition
The genetic code is universal
Term
A _______ is created when genes are transcribed and translated after being transplanted from one species to another
Definition
transgenic species
Term
RNA can be made from more ____
Definition
RNA
Term
The ______ to the template has the correct recipe for the protein in RNA language
Definition
complement
Term
RNA synthesis is catalyzed by _______ which pries the DNA strands apart and hooks together the RNA nucleotides
Definition
RNA polymerase
Term
Noncoding sequence DNA
Definition
is never converted into amino acid language
Term
The ____, ____, and _____ are noncoding sequences.
Definition
promoter, terminator, and introns
Term
Coding sequence DNA
Definition
DNA code that eventually creates polypeptide only in the transcription unit
Term
DNA Replication Needs:
Definition

helicase

3' OH group

primer/primase

Term
RNA Synthesis doesn't need help to:
Definition

pry open molecule(unzip)

3' OH group

primer/primase

Term
What are the three stages of transcription?
Definition
Initiation, elongation, termination
Term
Only this part of the molecule is made into the complementary strand of RNA
Definition
transcription unit
Term
What signal the initiation of RNA synthesis?
Definition
promoters
Term
The _____ box is the location of the promoter region
Definition
TATA
Term
______ continues to untwist the double helix, exposing about 10 to 20 DNA bases at a time for pairing with RNA
Definition
RNA polymerase
Term
In _______ only one protein is on the transcribed unit
Definition
eukaryotes
Term
In ______ up to four proteins are on the transcribed unit
Definition
prokaryotes
Term
What are the three stop codons?
Definition

UAG

UAA

UGA

Term
AUG is the ____ codon
Definition
start
Term
What are on the outskirts of the start and stop codons?
Definition
UTRs
Term
UTRs
Definition
un-translated regions on the outskirts of the start and stop codons
Term
Primary Transcript
Definition
initial molecule, the rough draft
Term
the Primary Transcrip RNA, _____, is modified in the eukaryotic nucleus
Definition
pre-mRNA
Term
The average length of a pre-mRNA molecule is _____nucleotides. The average protein size is ___ amino acids
Definition
8000....400
Term
What does the 5' end of pre-mRNA recieve?
Definition
Nucleotide cap
Term
What does the 3' end of a pre-mRNA recieve?
Definition
a poly-A-tail
Term
The nucleotide cap is made of...?
Definition
guanine and three phosphates
Term
What does the poly-A-tail do?
Definition
allows the pre-mRNA to leave the nucleus
Term
______ (TATA) on the gene
Definition
promoter
Term
____ (start) on transcript
Definition
AUG
Term
RNA Splicing removes ____ and joins ____
Definition
introns...exons
Term
After RNA Splicing occurs what is produced?
Definition
the final transcript! (mRNA)
Term
Spliceosomes
Definition
made up of snRNPs (snurps),RNA splicing enzymes
Term
Why is it important to be made of RNA? ex: enzymes, proteins
Definition
RNA attracts to other DNA/RNA=splicing
Term
Ribozymes
Definition
enzyme with RNA in it
Term
______ are catalytic RNA molecules that function as enzymes and can splice RNA
Definition
ribozymes
Term
Why is the # of possible proteins greater than the # of genes?
Definition
RNA Splicing!
Term
What determines an exon or intron?
Definition
Snurps determine it
Term
Proteins often have a __________
Definition
modular architecture
Term
What are the structural and functional regions in proteins?
Definition
domains
Term

mRNA is read: ____

tRNA is read: ____

Definition

5'-3'

 

3'-5'

Term
tRNA is full of anticodons/codons
Definition
anticodons
Term
How are there only 45 anticodons but 61 codons?
Definition
Sometimes an mRNA molecule will bind with the special base I
Term
tRNA
Definition
transfers amino acid to a ribosome, also translates codes
Term
tRNA carries a specific amino acid at the ____ end
Definition
3'
Term
tRNA code is _______ of mRNA code
Definition
complement
Term
______ causes tRNA to stick together
Definition
complementary base pairing
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