Shared Flashcard Set

Details

Ch. 1-3
Vocabulary
46
Microbiology
Undergraduate 2
09/25/2011

Additional Microbiology Flashcards

 


 

Cards

Term
Virus
Definition
No cell structure, parasitic, 20nm, DNA or RNA (not both)
Term
Bacterium
Definition
Prokaryotic, no internal compartments, 1-2um, single cell free living
Term
Protist
Definition
Eukaryotic, single cell, micro-millimeters
Term
Fungi
Definition
Eukaryotic, multicellular (except yeast), macroscopic with microscopic spores
Term
Helminths
Definition
Eukaryotic, multicellular, millimeters
Term
1 µm (micro)
Definition
= 10^(-6) m (meter)
= 10^(-3) mm (milli)
Term
1 nm (nano)
Definition
= 10^(-9) m (meter)
= 10^(-6) mm (milli)
Term
1000 nm (nano)
Definition
= 1um (micro)
Term
.001 um (micro)
Definition
= 1 nm (nano)
Term
Light Microscopy
Definition
light, glass lens, 1000X
res = .2 um (limited by wavelength)
Term
Bright field
Definition
fixed, stained, bright background
--light micro--
Term
Dark field
Definition
living, unstained, for shape/morphology
--light micro--
Term
Phase contrast
Definition
living, unstained, for internal detail
--light micro--
Term
3 type of light microscopy
Definition
Bright field
Dark field
Phase contrast
Term
Electron Microscopy
Definition
electron beam, electromagnetic coils
Term
2 types of Electron Microscopy
Definition
SEM
TEM
Term
SEM - Scanning Electron Micro
Definition
-fix, mount, coat in metal--
10,000X -- 3-D image of external detail
res = 3-20 nm
Term
TEM - Transmission Electron Micro
Definition
--fix, embed, cut section, stain--
100,000X - view internal detail
res = 2 nm
Term
Resolution
Definition
Ability to distinguish fine detail & structure
Term
Magnification
Definition
amount of times larger than actual size
Term
Total mag
Definition
= objective lens X ocular lens
Term
Resolution
Definition
= λ/2xNA

na of lens
Term
Simple stain
Definition
shape and arrangement
COLORFUL
Term
Simple stain protocol
Definition
C+ dye to neg cell, stain cell
Term
Differential stains
Definition
Acid fast & Gram stain
Term
Acid fast stain
Definition
C-
Shape, arrangement, presence of mycolic acids in cell wall
Term
Acid fast protocol
Definition
Carbolfuchsin, decolor, counterstain w/ Methylene Blue
Fast=PINK non=BLUE
Term
Gram stain
Definition
Shape, arrangement, biochemical properties
Term
Gram stain protocol
Definition
Crystal violet, mordant (iodine, decolor(ethanol), counterstain (Safranin)
Negative=RED Positive=PURPLE
Term
Bacilli
Definition
ROD
bacillus, diplobacilli, streptobacilli, coccobacillus
Term
Cocci
Definition
ROUND
coccus, diplococci, streptococci, staphylococci
Term
Spirilli
Definition
Vibrio - comma
Spirillum - squiggle
Spirochete - curly
Term
Diplo
Definition
Two
Term
Strepto
Definition
Chain
Term
Staphylo
Definition
Cluster (think grape bunch)
Term
Capsule
Definition
outside filmy layer (OPTIONAL)
made of: polysacc/sugar * polypept/protein
for attachment and prevention of phagocytosis
Term
Cell wall
Definition
2nd layer, 1st if no capsule (REQUIRED FOR BACTERIA)
made of: peptidoglycan/polymer
teichoic acid holds together, for shape (some osmotic control)
Term
Cell membrane
Definition
Innermost membrane (REQUIRED)
made of: lipid bilayer
selective permeability and osmotic protect
Term
Flagella
Definition
Long squirmy thing (OPTIONAL)
made of: flagellin/protein
for taxis (movement), rotary
Term
Pili
Definition
special fimbriae
transfers genetic info to other bacteria
Term
Fimbriae
Definition
Little hair like thing (OPTIONAL)
made of: pilin/protein
for attachment usually below waist infect
Term
Taxis
Definition
Movement/attraction
Term
Chemotaxis
Definition
movement/attraction to FOOD
Term
Phototaxis
Definition
movement/attraction to LIGHT
Term
Aerotaxis
Definition
movement/attraction to OXYGEN
Term
Osmotaxis
Definition
movement/attraction to WATER
Supporting users have an ad free experience!