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Cellular and Molecular Biology
Cellular and Molecular Biology
61
Biology
Undergraduate 2
10/15/2013

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Term
Plasma Membrane
Definition
Outer boundary of the cell that separates it from the world
Term
Compartmentalization (membrane function)
Definition
Membranes form continuous sheets that enclose intracellular compartments
Term
Scaffold/Biochemical activities (membrane function)
Definition
membranes provide framework that organizes enzymes for effective interaction
Term
Selectively Permeable Barrier (membrane function)
Definition
membranes allow regulate exchange of substances between compartments
Term
Transporting Solutes (membrane function)
Definition
membrane proteins facilitate the movement of substances between compartments
Term
Responding to External Signals (membrane function)
Definition
membrane receptors transduce signals from outside the cell in response to specific ligands
Term
Intracellular Interaction (membrane function)
Definition
membranes mediate recognition and interaction between adjacent cells
Term
Energy Transduction (membrane function)
Definition
membranes transduce photosynthetic energy, convert chemical energy to ATP, and store energy
Term
Importance of Lipid Bilayer
Definition
-composition can influence activity
-cohesion makes cells deformable and facilitates splitting and fusion of membranes
Term
Membrane Composition
Definition
-non covalent bonds
-membranes contain carbs as well
-protein:lipid ratios vary
Term
Amphipathic
Definition
containing both polar and non polar portions
Term
Phosphoglycerides
Definition
diglycerides with small functional groups linked to glycerol by phosphate ester bonds
Term
Sphingolipds
Definition
ceramides formed by the attachment of sphingosine to fatty acids
Term
Cholesterol
Definition
smaller and less amphipathic lipid only found in animals
Term
Integral Membrane Proteins
Definition
-penetrate and pass through bilayer
-20-30% encoded proteins
-amphipathic
Term
Peripheral Membrane Proteins
Definition
-attached to the membrane by weak bonds and easily solubilized
-entirely outside bilayer
Term
Lipid-anchored Membrane Proteins
Definition
-Glycophophatidylinositol (GPI)-linked proteins found on outer layer and can be released by inositol-specific phospholipases
Term
Ionic Sidedness (SDS)
Definition
denatures proteins
Term
Non-ionic Sidedness (Triton X-100)
Definition
does not alter proteins tertiary structure
Term
Site-directed Mutagenesis
Definition
-replaces specific amino acids with others
-Identifies some spatial relationships
Term
Fluorescence Recovery after Photobleaching (FRAP)+ Single Particle Tracking (SPT)
Definition
methods of labeling and tracking proteins
Term
Spectrin
Definition
-major component of the internal membrane skeleton
-attached by non covalent bonds
Term
Net Flux
Definition
-the difference between influx and efflux of materials
-can occur by passive diffusion and/or active transport
Term
Pore Domain
Definition
Permits selective passage of K+ ions
Term
Voltage Sensing Domain
Definition
Helices S1-S4 and senses voltage across the plasma membrane
Term
Co-transport
Definition
Coupling active transport to existing ion gradients
Term
Secondary Transport
Definition
the use of energy stored in an ionic gradient
Term
Resting Potential
Definition
the membrane potential of a nerve or muscle cell, subject to changes when activated
Term
Action Potential
Definition
when cells are stimulated, Na+ channels open, causing membrane depolarization
Term
Membrane Carbohydrates
Definition
-covalently linked to lipids and proteins extracellularly
-glycoproteins have short, branched carbs for interaction with other cells
-glycolipids have larger carbohydrate chains that may be cell-to-cell recognition sites
Term
Virion
Definition
a virus particle outside of a host cell
Term
The first step of glycolysis is facilitate by the enzyme ....
Definition
Hexokinase
Term
Fructose 1,6-bisphosphate
Definition
The compound that is cleaved into 2 three carbon molecules to synthesize pyruvate and ultimately ATP
Term
Uracil is formed from cytosine via a ...
Definition
Deamination reaction
Term
What kind of organism reaches equilibrium
Definition
A dead organism
Term
3 amino acids involved primarily in kinase reactions
Definition
Serine, Threonine, Tyrosine
Term
Proline
Definition
"Helix breaker" amino acid, due to its inflexible R-Group
Term
Components of a nucleotide
Definition
Adenine, Ribose, and a phosphate
Term
Aerobes
Definition
an organism that can survive and grow in an oxygenated environment
Term
Inner Boundary Membrane (mitochondrion)
Definition
forms internal compartments known as cristae, which allow greater space for the proteins such as cytochromes to function properly and efficiently
Term
Cristae
Definition
a fold in the inner membrane of a mitochondrion
Term
Mitochondrial Matrix
Definition
contains the mitochondria's DNA and ribosomes
Term
Outer Mitochondrial Membrane
Definition
has a protein-to-phospholipid ratio similar to the eukaryotic plasma membrane. It contains numerous integral proteins called porins, which contain a relatively large internal channel that is permeable to all molecules of 5000 daltons or less
Term
Intermembrane Space
Definition
the region between the inner membrane and the outer membrane of a mitochondrion or a chloroplast. The main function of the intermembrane space is oxidative phosphorylation
Term
Porin
Definition
beta barrel proteins that cross a cellular membrane and act as a pore through which molecules can diffuse
Term
Glycolysis
Definition
the metabolic pathway that converts glucose C6H12O6, into pyruvate, CH3COCOO− + H+
Term
Tricarboxylic Acid Cycle (TCA)
Definition
a series of chemical reactions used by all aerobic organisms to generate energy through the oxidation of acetate derived from carbohydrates, fats and proteins into carbon dioxide and chemical energy in the form of adenosine triphosphate (ATP)
Term
Chemiosmosis
Definition
the movement of ions across a selectively permeable membrane, down their electrochemical gradient
Term
Oxidative Phosphorylation
Definition
the metabolic pathway in which the mitochondria in cells use their structure, enzymes, and energy released by the oxidation of nutrients to reform ATP
Term
Redox Reactions
Definition
all chemical reactions in which atoms have their oxidation state changed—that is, redox reactions involve the transfer of electrons between species.
Term
Electron Transport Chain
Definition
couples electron transfer between an electron donor (such as NADH) and an electron acceptor (such as O2) with the transfer of H+ ions (protons) across a membrane
Term
Flavoproteins
Definition
involved in a wide array of biological processes, including, but by no means limited to, bioluminescence, removal of radicals contributing to oxidative stress, photosynthesis, DNA repair, and apoptosis; polypeptides bound together by FAD or FMN
Term
Cytochromes
Definition
membrane-bound (i.e. inner mitochondrial membrane) hemeproteins containing heme groups and are primarily responsible for the generation of ATP via electron transport; contain heme groups bearing Fe or Cu ions
Term
Three cooper atoms
Definition
located within a single protein complex and alternate between Cu2+ and Cu3+
Term
Ubiquinone
(coenzyme Q)
Definition
a lipid-soluble molecule made of five carbon isoprenoid units
Term
Iron-sulfur proteins
Definition
contain Fe in association with inorganic sulfur
Term
Cytochrome Oxidase
Definition
a large complex that adds 4 electrons to O2 to form two molecules of H2O
Term
Proton-motive force
Definition
energy present in both components of the gradients
Term
pH Gradient
Definition
Concentration gradient between matrix and intermembrane space
Term
Peroxisomes
Definition
Membrane bound vesicles that contain oxidative enzymes
Term
Glyoxisome
Definition
Special type of peroxisome specific to plants that can convert fatty acids to glucose by germinating seeds
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