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Cell Fizz 1
chemical and enzymatic mechanisms for polypeptide cleavage
15
Biology
Undergraduate 4
09/06/2010

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Term
Sanger's Reagent (FDNB: Flourodinitrobenzene)
Definition
N-terminal residue
Term
Dansyl Chloride
Definition
N-terminal residue
Term
Edmand degradation (phenylisothiocynate)
Definition
N-terminal residue
Term
Cyanogen Bromide
Definition
COOH side of Met (Methionine)
Term
O-Iodosobenzoate
Definition
COOH side of Trp (Tryptophan)
Term
Hydroxylamine
Definition
Asn-Gly bonds (asparagine-Glycine only in this order)
Term
2-Nitro-5-thiocyanobenzoate "2-5"
Definition
NH2 side of Cys (Cysteine) "NH2"
Term
Hydrazine
Definition
"C" terminal
Term
Lithium Aluminumhydride
Definition
"C" terminal
Term
Pepsin
Definition
COOH side of Phe and Leu (Phenylalanine and Leucine)
Term
Trypsin
Definition
COOH side of Lys and Arg (Lysine and Arginine)
Term
Clostripain
Definition
COOH side of Arg (Arginine)
Term
Staphloccocal Protease
Definition
COOH side of Asp (Aspartic Acid)
Term
Chymotrypsin
Definition
COOH side of Phe, Tyr, and Trp (Phenylalinine, Tyrosine, Tryptophan)
Term
Carboxypeptidase
Definition
C terminal only: best if C residue is Bulky or aromatic. Won't work if C is preceded by Pro (Proline)
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