Term
| Stability depends on (3 things) |
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Definition
1. The amount of free tubulin subunit
2. The rate of hydrolysis
3. GTP cap |
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Term
Destabilizing Proteins
Stathmin |
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Definition
| A tublin dymer that sequesters the free tublin in the cytoplasm. It promotes disassembly |
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Term
| Katanin- severing protein |
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Definition
| An ATPase that severs the MT in the middle. It uses the energy of ATP to disrupt end to end assocaiton. |
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Term
| Kinesin 13- a motor and destabilizing protein |
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Definition
| it binds to + ( beta) and pryse the protifilaments apart |
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Term
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Definition
MAP2 MAPs: it needs to be in its de(P) state in order to the side of the MT. It helps stablize structure.
Many disorder have (P) and have problems de(p)
eg. Alzheimer's |
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Term
| + Tip ( Stabilizing proteins) |
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Definition
they bind to the + end
they add tubulin to the + end and acts as a polymerase.
This helps in stabilization |
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Term
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Definition
| they bind to MT and they will move diffrent cargo in either the + or - direction |
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Term
Kinesin
Stucture and Function |
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Definition
-it is going to move cargo towards the + end
-they are composed of two heavy chains and two light chains
-they have four domains 1. Motor domain which are globular in nature
2. The stalk which is very flexiable
3. Short neck region between the head and the stalk
4. The c-terminal domani which is going to bind with the ligh chain( binds the cargo)
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Term
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Definition
| Adapter proteins and receptors on the vesicles |
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Term
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Definition
1. Take a slide and attach MT tracks
2. Use MT with know MOTC such as centersome
3. Use motor proteins with hybrid beads attached with a laser. |
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Term
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Definition
They move 8nm, they walk hand on hand in a progressive fashion
The important thing is to co-ordinate the ATP cycles of hydrolysis. No nucleotide leads to tight binding as is ATP and ADP is weak binding.
Both will be bound to ADP then on ADP gets relaesd and an ATP comes on which sends the other hand forward, as soon as that hand hits the MT it will hydrolyisis its ADP and then the other one will kinase its ADP to ATP. |
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Term
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Definition
this motor protein will move towards the minus end ( towards the MOTC)
- giant ATPase: cytoplamic and axonemal |
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Term
cytoplasmic
dynei stucture |
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Definition
| 1. A domain that binds to the MT 2. a short stalk ( coiled stalk) 3. large motor protein 4. long stem which connects to 5. C-terminus |
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Term
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Definition
multi protein complex that is goin t o connect o light and heacy and intermediat chains they help bind to cargo
The motor heads consists of 6ATPase and only one domain will hydrolyze the ATP |
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Term
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Definition
| to bind to the MT we need to be in an open state or no nt bound. NO MT is very tight binding, then ATP will bind, ATP will be hydrolyzed and we get immedaite relase of the ADP +pi. But the hydrolization is going to casue a counterclockwise shift, which is gonig to shift the stalk while the stem stays the same: this is gonig to lead to angular displacment. |
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