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Bridgemont microbiology chapter 3
Bridgemont microbiology chapter 3
73
Microbiology
Undergraduate 1
02/04/2013

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Term
Bacteria 
Definition
Prokaryotes
Term
Animal, plant, fungi, protozoa
Definition
Eukaryotes
Term
Prokaryotes usually considered much more simple in from compared to Eukaryotes 
Definition
True ( they where considered simple but they are not truely. They are only simpler in their structure not their function )
Term

Both cell types depend on homeostasis for cellular survival

(True/false)

Definition
True
Term

Organisms ability to maintain a stable I internal balance (internal stability)

regulated by metabolic (chemical) or stuctural change

-adapt or die 

Definition
Homeostasis
Term
Both call types communicate with each other cells a d respond to:
Definition
Stimuli
Term
Plant cells shrinking in response to lack of water is an example of?
Definition
Eukaryotes stimuli
Term
Animal producing sound to communicate is an example of?
Definition
Eukaryotes communication
Term
Prokaryotes forms micro-communities called?
Definition
Bioflims
Term
Collection of bacteria microorganisms living and interacting in a " multicellular state"
Definition
Biofilms
Term
5 steps to biofilm formation/ life cycle
Definition

1) reversible attachment

2) irreversible attrachment

3) maturation 1

4 maturation 2

5) dispersion 

Term
Prokaryotes within in a biofilm communication through?
Definition
Quorum sensing
Term

The ability for bactetia to produce and release chemical signals. 

-allows for "cell-cell communication."

-communicate and coordinate behaviors

Definition
Quorum sensing
Term

Singcall ell organisms in effect become multicellular organisms and can act together.

-"multicellular start"; simulate multicellular functions 

Definition
Quorum sensing
Term
Examples of harmful biofilms ( disease causing)
Definition

plaque

some lung/ ear infections

Term
examples of useful biofilms 
Definition

Sewage treatment

bioremediation: clean up of chemically contaminated environments 

Term
Prokaryotes/Eukaryotes are both surrounded by outer?
Definition
Cell menebrane (know as plasma menebrane in  Eukaryotes
Term
Allows fluid and waste to exchange between the cell and its environment 
Definition
Cell membrane 
Term
Prokaryotes/Eukaryotes both allow for _____ within individual cells
Definition
Metabolic process
Term
Both Prokaryotes/Eukaryotes preform ______ to produce stucntural and functional parts of the cell
Definition
Protein synthesis
Term
Both Prokaryotes/Eukaryotes make proteins by using_____.
Definition
Ribosomes
Term
Both Prokaryotes/Eukaryotes have ____ as genetic material 
Definition
DNA
Term
DNA is genetic material in _____.
Definition

Chromosomes

(chromosomes structure and # vary)

Term
No membrane bound nucleus/organelles present
Definition
Prokaryotes
Term
DNA in nucleoid; region/location of genetic material inside cell w/in cytoplasm
Definition
Prokaryotes
Term

DNA; single, circular strand

(double strand helix)

Definition
Prokaryotes
Term
Menebrane bounds organelles w/in cytoplasm
Definition
Eukaryotes
Term
DNA in nucleus ( menebrane bound)
Definition
Eukaryotes
Term
DNA; multiple, linear strands (double bound)
Definition
Eukaryotes
Term

Eukaryotes cell approx 10 X larger than  Prokaryotes


true/false

Definition
True
Term
Prokaryotes chromosome;
Definition
Single circular
Term
Eukaryotes chromosomes are;
Definition
Multiple linear 
Term
Metabic processes; both Prokaryotes/Eukaryotes perform_____.
Definition
Cellular respiration
Term
Cells convert chemical energy into usable cellular energy  is
Definition
Cellular respiration 
Term
metabolic process: with in Eukaryotes cellular respiration take place
Definition
In mitochondria (organelle) and /or chloroplasts (organelle in photosynthetic)
Term
Metabolic processes: within Prokaryotes takes place in
Definition

In cytosol (liquid inside cell within Cytoplasm)

along cell membrane 

Term
Is science of classifying organisms based on natural or logical relationships. 
Definition
Taxonomy
Term
Historically living organisms including bacteria were grouped according to______.
Definition
Visual similarities 
Term
Laid the foundation for modern taxonomy
Definition
Carolus Linnaeus
Term
Carolus Linnaeus devised a 2 name system to classify organisms: it was called
Definition
Binomial Nomenclature
Term
Two words used to classify an organism
Definition
Genus/ species
Term
Classification rank compose of a group of one or more species of organisms with similar characteristics. ( used alone or with species/ specific epithet name. 
Definition
Genus
Term
Further describes the genus name; least inclusive classification rank; based on morphological and biological similarities. 
Definition
Species
Term
Used classify organisms based on successive leveLos if similarities 
Definition
Hierarchical System of classification 
Term
Old Hierarchical system of classificatio
Definition

-domain

- kingdom

- Phylum

-class

- order 

- family

-genus

- species 

Term
The 3 domain system
Definition

- Eukarya (plantea, animal, fungi, Protista)

- Bacteria ( eubacteria, true bactetia, old)

- Archaea ( extre bacteria )

Term
5 kingdom systems
Definition

-animal

-fungi

-plantea

-protista

-monera ( bacteria)

Term
Using staining reaction to determine morphology of cells   (Shape, size and arrangement)
Definition
Physical characteristics 
Term

Look at metabolic processes used different species

-Use a narrow down potential pathogens

Definition
Biochemical test
Term
Testing of blood serum/watery part; antigen-antibody interaction
Definition
Serological Test
Term

Look and compares bacterial ribosomal RNA (rRNA)

-basis for the separation of the bacteria for on the Archaea 

Definition
nucleic acid analysis
Term

A method of determining the identity of an unknown species used a series of positive or negative test results/ procedures

- is not an individual test

Definition
Dichotomous key
Term
Why use stain to see bacteria?
Definition

-because bacteria cells are very tiny and colorless

-diffical to see contrast between cell and backgrown of the microscope field

Term

-Make a wet mount of specimen

-Heat fix specimen

-Add Basic Stains (cationic dye)(only one stain used)

 

Definition
Simple Stain Technique
Term

-Opposite of simple stain

-Negatively charged acidic (anionic) dye used

-No heat fixation required (room temp)

Definition
Negative stain technique
Term

Allows cells to be differentiated visually into groups based on staining differences

-use 2 stains and a decolorizer

Definition
differential staining technique
Term
the method is named after its inventor, the Danish scientist Hans Christian Gram
Definition
gram stain technique
Term

-prepare sample/ smear-air dry-heat fix

-Crystal Violet 1 min-rinse

-Gram's iodine 1 min-rinse

-Decolorizer 10-15 sec-rinse

-Safranin 1 min-rinse

view results under oil immersion

Definition
Steps for gram stain technique
Term

Purple cells

thick cell wall retains original stain

Definition
Gram +
Term

red cells

thin cell wall releases primary stain and requires a counterstain for viewing

Definition
Gram -
Term
Cells retain red/pink prink primary stain after acid-alcohol wash
Definition
Acid Fast
Term
Cells appear blue following acid-alcohol wash
Definition
Non Acid Fast
Term
Another physical characteristic for studying microorganisms and viruses
Definition
size
Term

Based of the metric system

-Standard unit of length is the_______.

Definition
meters (m)
Term

Micrometer (um)

1um=

Definition
one millionth of a meter or .000001
Term
Bacterial size range
Definition

100 um= very large cells

1um = small cells

Term
Majority of bacterial cells are
Definition

1um - 5um

(blood or spore cells are approx .10um)

Term

virus size measured in

 

Definition
Nanometers (nm)
Term
primary tool used to observe microorganisms?
Definition
Microscope
Term

uses visible light rays and convex lenses

-used to increase magnification

Definition
light microscope/compound microscope
Term

used to inrease resolving power of micreoscope

- ability to view closely spaced objects clearly

Definition
oil immersion
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