Term
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Definition
cloning a segment of DNA of interest
- cut vector with a restriction enzyme
-add ligase to add sequence of interest
-put the recombinant DNA in a bacterial cell (transformation)
-Amplify the DNA- make multiple copies in a culture
-Isolate the recombinant DNA
-Cut with restriction enzymes |
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Term
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Definition
| Carrier molecules that are used in DNA cloning, self replication DNA molecules that are used to transfer foreign DNA segments between host cells |
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Term
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Definition
| -small circular DNA molecules that exist in the cytoplasm of bacterial cells |
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Term
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Definition
| -origin of replication, allows for replication of plasmid DNA, a selectable marker- allows for selection for cells that have plasmid DNA |
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Definition
| target for a restriction enzyme- where the insert will be inserted |
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Term
| Screening for bateria with recombinant DNA |
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Definition
| pBR322 vector: adding the insert disrupts a gene that otherwise would provide tetrocyclin resistance. Cells carrying recombinant DNA die in the presence of tetracycline maintain ampR |
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Term
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Definition
adding insert dirsupts the lacZ gene. Lac Z encodes beta-galactosidase
X-Gal--> (beta gal) X + gal
want the white ones, not the blue |
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Term
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Definition
contains a collection of recombinant DNA that is transcribed gene of interest
-copy of a fully processed mRNA |
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Definition
Gene transfered into somatic cells of the body; single target tissue
-not passed on to the next generation
-doesn't last |
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Term
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Definition
-destroying of white blood cells leads to a severe immune deficiency
-Bone marrow transplant is a cure- but could cause an attack on the patients tissue if not a match |
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Definition
-Genetic engineering of organisms
-add new gene that is incorporated into the all of the organisms cells
-Change passed on to the next generation |
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Term
| Modification in embryonic cells |
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Definition
- produces a chimera- not all cells have the same genotype
-inject cells into the early embryo |
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Term
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Definition
| - add gene to the male pronucleus before sygamy( where the cell is feterilized) |
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Term
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Definition
-undifferentiated cells (callus)
-add in particular thing to the plant
-bacterium uses a tumor (Ti) vector to introduce trans-gene |
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Term
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Definition
| - can give rise to all the cell types in the adult body and cells in supportive tissues- to the whole organism |
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Definition
| Present in the inner cell mass- able to give rise to all of the cell types in the body but cannot form an individual |
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Term
| multipotent (adult stem cells-somatic stem cells) |
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Definition
| Present in many tissues- tissue specific |
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