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Blood Bank Exam 3 Lecture 19 & 20
Compatibility Testing I & II
87
Biology
Undergraduate 3
02/21/2015

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Term
True or False:
Lactated Ringer's, Injection (USP) or other electrolyte solutions containing calcium should NEVER be added to or administered concurrently with blood or components collected in an anticoagulant containing citrate
Definition
True
Term
Blood and components must be inspected immediately prior to use. If upon visual inspection the container is not intact or the appearance is abnormal,do you use the blood?
Definition
No, it must NOT be used for transfusion
Term
What must the request form from the patient's floor have on it at minimum (3-4)?
Definition
First and last name of patient, identification number, and the physician name
Term
True or False:
Incomplete or illegible requests should NOT be accepted
Definition
True
Term
What must the request form provide to ensure that the patient receives the right specimen (2)?
Definition
What product is required
Date of transfusion
Term
Though verbal or phone orders are acceptable, the orders must be back up later with what?
Definition
Requisition
Term
If a patient has had pregnancy or transfusion within the last three months, the sample must be obtained within how many days of the scheduled transfusion to assure current immunologic status?
Definition
3 days
Term
Benefit of Serum over Plasma?
Definition
Serum does not contain fibrin strands which can be confused for agglutination
Term
Some labs add a drop of what to accelerate clotting?
Definition
Thrombin
Term
Benefit of EDTA Plasma over Plasma?
Definition
EDTA Plasma will not contain complement as calcium is needed for its activation
Term
True or False:
Some ABYs require C' to be detected, such as Jk^a
Definition
True
Term
What type of component should you use in order to have C' available?
Definition
Fresh Serum
Term
Why should you avoid hemolyzed samples?
Definition
May interfere with the detection of hemolysis from AGN/ABY reaction
Term
What must the tube that is labeled at the bedside contain at minimum?
Definition
Name
ID #
Date
ID of phlebotomist
Term
If the information on the tube does NOT match the requisition, what must happen?
Definition
The discrepancy must be resolved before continuing
Term
Tubes must be kept at refrigeration for a minimum of how many days in order to make there are no delayed transfusion reactions?
Definition
7 days
Term
How often should you collect a new sample from a patient?
Definition
Every 3 days
Term
True or False:
Patient records must be reviewed and documented
Definition
True
Term
What should you look for when reviewing a patient's record?
Definition
Blood type
Previous ABYs
Transfusion Reactions
Typing discrepancies
Term
If a patient had an ABY before, what type of units must you give them now?
Definition
AGN Negative units
Term
ABY testing must be performed at what temperatures?
Definition
37 degrees Celsius & AHG
Term
You must add check cells to what kind of tubes?
Definition
All negative tubes
Term
When trying to rule out AGNs, you want to use screening cells which have most of the AGNs in what form?
Definition
Homozygous
Term
ABY testing can be performed prior to and concurrently with what?
Definition
Crossmatch
Term
True or False:
If you use anti-IgG AHG, be sure to use screening cells that are homozygous for Kidd
Definition
True
Term
Why should you use screening cells that are homozygous for Kidd if you use anti-IgG AHG?
Definition
Kidd might only show up with C'
Term
If the IAT is performed correctly and is Negative, what is the percent chance that the crossmatch will be compatible?
Definition
99%
Term
When selecting blood to give to a patient what needs to be specific if possible?
Definition
ABO
Term
If the blood is not specific, what type of blood should be given?
Definition
Group compatible
Term
What is the acceptable Donor Cells for the following Patient's Blood:
A
Definition
A
O
Term
What is the acceptable Donor Cells for the following Patient's Blood:
B
Definition
B
O
Term
What is the acceptable Donor Cells for the following Patient's Blood:
AB
Definition
AB
A
B
O
Term
What is the acceptable Donor Cells for the following Patient's Blood:
O
Definition
O
Term
What is the acceptable Plasma type for the following Patient's Blood:
A
Definition
A
AB
Term
What is the acceptable Plasma type for the following Patient's Blood:
B
Definition
B
AB
Term
What is the acceptable Plasma type for the following Patient's Blood:
AB
Definition
AB
Term
What is the acceptable Plasma type for the following Patient's Blood:
O
Definition
O
A
B
AB
Term
What is the acceptable Rh type for the following Patient Rh:
Rh+
Definition
Rh +
Rh =
Term
What is the acceptable Rh type for the following Patient Rh:
Rh = Weak D =
Definition
Rh =
Term
What is the acceptable Rh type for the following Patient Rh:
Weak D +
Definition
Rh +
Rh =
Term
Define Major Crossmatch
Definition
Mix DONOR RBC with PATIENT SERUM
Term
True or False:
The major crossmatch may detect ABY in patient's serum that was not detected by the IAT due to ABY against a low incidence AGN not on screening cells
Definition
True
Term
True or False:
Major crossmatch will not always show agglutination even if patient IAT was positive
Definition
True
Term
Why will the Major crossmatch not always show agglutination even if patient IAT was positive?
Definition
The unit could lack the AGN
Term
What temperature is sufficient ONLY if the IAT is NEGATIVE AND the patient has NO history of previous significant ABY?
Definition
RT
Term
If IAT is POSITIVE or there is a history of ABY, incubate at what temperature and test through what?
Definition
37 Degrees Celsius
AHG
Term
What type of units do you give to a patient even if the crossmatch is now Compatible?
Definition
AGN Negative Units for those with ABYs
Term
What will the Major Crossmatch NOT detect or prevent?
Definition
ALL unexpected ABYs
Anamnestic Reactions
Rh errors
ALL ABO errors
Term
Define Minor Crossmatch
Definition
PATIENT CELLS with DONOR SERUM
Term
True or False:
Major Crossmatch is no longer performed since ABY screen is performed on donors
Definition
False
Term
True or False:
Minor Crossmatch is no longer performed since ABY screen is performed on donors
Definition
True
Term
What is the criteria to use Computer Crossmatch in place of RT/IS?
Definition
*Use only when ABO compatibility is required
*2 Independent ABOs on the patient
*CPU contains donor information
Term
Computer Crossmatch must be able to alert user to what?
Definition
discrepancies between donor and labeling
Confirmation of type and to ABO incompatibilities between donor and recipient
Term
What are the 2 examples for 2 Independent ABOs on a patient?
Definition
*1 on current sample and 1 by testing same sample by different technician
*1 on current sample and comparison with previous records
Term
What 7 things are needed on the label on blood unit?
Definition
Donor ID
ABO
Rh
Results of crossmatch
Initials of Tech
Date of Testing
Expirations of component
Term
Issuing blood to nursing staff is an elaborate system to check and double check all information to prevent what?
Definition
Clerical Errors
Term
All information is checked where against the requisition?
Definition
BB
Term
All information is checked again at patient bedside by whom?
Definition
2 nurses who sign form
Term
Name 7 Typing Problems
Definition
*Weak subgroups with or without an Anti-A1
*Acquired AGNs
*Nonspecific agglutination
*Mixed cell populations
*Disease states
*Immunosuppression
*Old Age
Term
What do you use to determine if there are weak subgroups with or without an Anti-A1
Definition
Lectin
A2 cells
Term
What do you need to help determine if a patient has acquired AGNs?
Definition
Good History
Term
If Everything agglutinates what do you do?
Definition
Wash in large amounts of saline
Term
What is one cause of everything agglutinating?
Definition
Wharton's jelly
Term
What can cause Mixed cell populations?
Definition
Previous transfusion with different blood group
Term
The following are 3 examples of what?
A group given O
Chimera
BMT
Definition
Mixed Cell populations
Term
What do disease states weaken?
Definition
A or B AGNS
Term
What are 2 things that can weaken ABYs?
Definition
Immunosuppression
Old Age
Term
What are 5 Causes for Incompatible crossmatches
Definition
Rouleaux
Technical Problems
Typing Problems
Alloantibodies
Autoantibodies
Term
How do you detect Alloantibodies?
Definition
IAT and/or previous records
Term
IgM, IgG, or undetermined specificity are 3 causes or types of what?
Definition
Alloantibodies
Term
If a patient has autoantibodies what will be positive in the donor?
Definition
DAT
Term
The following are what type of ABYs?
I, i, H, or IH
Definition
Cold autoantibodies
Term
Name 6 examples of technical problems
Definition
Dirty glassware
Bacterial contamination
Fibrin
Contamination of saline
Spinning
RBC suspension
Term
If a patient has chosen autologous blood what must you confirm?
Definition
ABO
Rh
Term
If a patient has chosen autologous blood what is optional to confirm?
Definition
IAT
Crossmatch
Term
For Intrauterine what must the blood be (4)?
Definition
Less than 5-7 days old
O Negative
Irradiated
CMV Negative
Term
True or False:
Under certain special circumstances you can type and screen instead of crossmatch because the crossmatch will be performed prior to issuing the blood later
Definition
True
Term
Explain the following:
+ IAT
= Auto
+ Major
Definition
Recipient has Allo that is also on donor cells
Term
Explain the following:
= IAT
= Auto
+ Major
Definition
If RT, check ABOo
If AHG check donor DAT or low incidence ABY in patient
Term
Explain the following:
+ IAT
+ Auto
+ Major
Definition
Auto or an Allo reacting with previously transfused cells
Rouleaux
Term
Explain the following:
Anti-A =
Anti-B =
A1C =
BC +
A2C =
IAT =
Definition
Ax
Term
Explain the following:
Anti-A +
Anti-B +
A1C +
BC +
A2C NT
IAT =
Definition
Polyagglutination
Term
Explain the following:
Anti-A 4+
Anti-B +
A1C =
BC +
A2C NT
IAT =
Definition
Acquired B
Term
Explain the following:
Anti-A =
Anti-B 4+
A1C =
BC =
A2C =
IAT =
Definition
Weak Anti
Term
Explain the following:
Major =
Minor =
IAT +
DAT =
Definition
Units lack AGN of patient
Term
Explain the following:
Major =
Minor +
IAT =
DAT +
Definition
Patient with + DAT
Term
Explain the following:
Major +
Minor =
IAT =
DAT =
Definition
Rare ABY or Donor with + DAT
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