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| the process of producing populations of genetically-identical individuals that occurs in nature when organisms such as bacteria, insects or plants reproduce asexually. |
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| recombinant DNA technology |
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Definition
A novel procedure of cloning or subcloning of any DNA fragment by inserting the special DNA fragment of interest into a special area of target DNA through interchange of the relevant DNA fragments.[1] This is a one-step reaction: simple, efficient, facilitating high throughput or automatic cloning and/or subcloning |
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| an enzyme that cuts double-stranded or single stranded DNA at specific recognition nucleotide sequences known as restriction sites. |
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| a form of synthetic DNA that is engineered through the combination or insertion of one or more DNA strands, thereby combining DNA sequences that would not normally occur together. |
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polymerase chain reaction (PCR) |
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Definition
| a technique widely used in molecular biology. It derives its name from one of its key components, a DNA polymerase used to amplify a piece of DNA by in vitro enzymatic replication. |
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| a technique employed by forensic scientists to assist in the identification of individuals on the basis of their respective DNA profiles. |
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| an organism whose genetic material has been altered using genetic engineering techniques. These techniques are generally known as recombinant DNA technology. |
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manufactured by recombinant DNA technology, one where genetic manipulation of cells is required, or a monoclonal antibody |
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Definition
| the study of an organism's entire genome. The field includes intensive efforts to determine the entire DNA sequence of organisms and fine-scale genetic mapping efforts. The field also includes studies of intragenomic phenomena such as heterosis, epistasis, pleiotropy and other interactions between loci and alleles within the genome. |
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Definition
| the large-scale study of proteins, particularly their structures and functions |
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| the application of information technology to the field of molecular biology. Bioinformatics entails the creation and advancement of databases, algorithms, computational and statistical techniques, and theory to solve formal and practical problems arising from the management and analysis of biological data. |
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Definition
| the insertion of genes into an individual's cells and tissues to treat a disease, and hereditary diseases in which a defective mutant allele is replaced with a functional one |
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