Term
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Definition
Phenylalanine deaminase
tests for phenylalanine deaminase production, digests phenylalanine
positive plate turns green
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Term
|
Definition
Urease Plate
positive turns pink |
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Term
|
Definition
Eosin Methylene Blue
green metalic- ecoli, lactose fermentation
black- lactose fermentation
clear- negative
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Term
|
Definition
red butt and slant: no fermentation
yellow butt and red slant: glucose fermentation
yellow butt and slant: lactose or sucrose fermentation
black butt and red slant: glucose ferment and H2S
black butt and yellow slant: lactose/sucrose fermentation and h2s
gas or no gas?
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Term
What is Eosin Methylene Blue selective for and why?
|
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Definition
Selective for gram negative bacteria
Methylene Blue toxic to gram positive |
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Term
| How is Eosin Methylene blue differential? |
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Definition
Lactose fermentaion:
colonies positive for lactose fermentation are black/ or green
non fermenters are clear |
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Term
| What is TSI and what does it test for? |
|
Definition
Triple Sugar Iron slant
tests for glucose fermentation
lactose/sucrose fermentation
H2S (hydrogen sulfide)
and gas production |
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Term
| what produces the black substance and gas in TSI slants |
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Definition
black: thiosulfate is converted to hydrogen sulfide
gas: thiosulfate to hydrogen gas
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Term
| what is the reaction that urease catalyzes |
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Definition
tests for urease
reaction: urea + water ---> ammonia c02
(NH2)2CO + H2O → CO2 + 2NH3 |
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Term
|
Definition
Mannitol Salt Agar
yellow is positive for mannitol fermentation
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Term
| what bacteria is Mannitol Salt Agar selective for? |
|
Definition
| High salt concentrations, selects for gram positive staphylococcus |
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Term
|
Definition
|
|
Term
What does each tube indicate? What test?
[image] |
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Definition
Sulfide Indole Motililty test
A) uninoculated
B) non motile and indole negative
C) Motle and indole positive
D) motile, indole positive, H2S producing |
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Term
| What must be added to SIM to test for indole? |
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Definition
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|
Term
What test is this? Which is positive
[image] |
|
Definition
Citrate test
can organism use citrate as sole carbon source? Blue is positive, can metabolyze ammonium salts |
|
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Term
What test? Positive and negative?
[image] |
|
Definition
MacConkey
Pink colonies Lac+
Clear colonies Lac -
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Term
| What is selective/ differenctial for MacConkey agar? |
|
Definition
selective for gram negative, contains bile salts and crystal violent
differential for lactose fermentation |
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|
Term
What plate is this and what does it show?
[image] |
|
Definition
Blood Agar
shows
Beta, Alpha, and Gamma hemolytic streptococcus |
|
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Term
| What are the four kinds of flagella arangements? |
|
Definition
[image]
Monotrichous
Lophotrichous
Peritrichous
Amphitrichous |
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Term
|
Definition
Methylene blue
stains all cells |
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Term
|
Definition
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Term
|
Definition
|
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Term
| How is the gram stain done? |
|
Definition
Apply crystal violet
set with iodine
decolorize with ethyl alcohol
apply safranin
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Term
| what does the gram stain differentiate for? |
|
Definition
peptidoglycan wall
thick in gram positive, retains crystal violet
thinner in gram negative, more lipid content in outter cell membrain gets washed away by alcohol |
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Term
What bacteria is acid fast used for?
what dye is used? |
|
Definition
Mycobacterium tuberculosis
kinyoun stain
acid alcohol
methylene blue |
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Term
|
Definition
2 H2O2 → 2 H2O + O2
hydrogen peroxide -> water and oxygen |
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Term
|
Definition
Kirby-Bauer test
zone of inhibition around antibiotic discs |
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|
Term
[image]
What test? what is a positive result? |
|
Definition
Starch Hydrolesis
Can bacteria break down starch?
Positive for amylase, clear zone when iodine is added. |
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|
Term
name the morphologies
round
rod
oval
chains
pairs
clusters |
|
Definition
coccus
bacillus
coccobacillus
stepto-
diplo-
staphylo- |
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Term
[image]
what test and what is A, B, C
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|
Definition
CAMP TEST
testing for camp factor
arrow is zone of enhanced hemolysis
A) Beta Streptococcus group B
B) Beta Streptococcus group A
C) Staphylococcus aureus
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Term
| What are the three groups of strep? |
|
Definition
Beta- streptococci
Viridans streptococci
Pneumococci |
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Term
| what pathogen causes pneumonia |
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Definition
|
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Term
| Which Beta steptococcus group is succestible to Bacitracin? |
|
Definition
Group B
group A is immune |
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Term
|
Definition
| latex agglutination test, latex coverered in antibodies that recognize different groups of streptococcus |
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|
Term
Streptococcus pneumoniae
catalase
Optochin
hemolysis
gram reaction
morphology
arangement
|
|
Definition
catalase negative
optochin sensitive
alpha hemolysis
gram positive
coccus
chains
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|
Term
Streptococcus mutans
catalase
Optochin
hemolysis
gram reaction
morphology
arangement
|
|
Definition
catalase negative
optochin resistant
hemolysis alpha
gram positive
coccus
chain |
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|
Term
| Coagulase turns what to what, used to differentiate what? |
|
Definition
|
|
Term
Beta Strep Group A
Catalase
Bacitracin
CAMP
Hemolysis
Gram
|
|
Definition
catalase negative
Bacitracin resistant
CAMP negative
hemolysis beta
gram positive |
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|
Term
Beta Strep Group B
Catalase
Bacitracin
CAMP
|
|
Definition
catalase negative
Bacitracin susseptible
camp positive |
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|
Term
Staphylococcus
gram?
catalase?
|
|
Definition
gram positive
catalase positive |
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|
Term
what tests differentiate Staphylococcus
aureus, epidermidis
saprophyticus |
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Definition
| Novobiocin, Manitol Salt Agar, Coagulase, DNAse, Hemolysis |
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Term
| Which Staph strains are susceptable to Novobiocin |
|
Definition
|
|
Term
| Which staph are susceptible to Novobiocin |
|
Definition
|
|
Term
What are the hemolytic properties
of
s. aureus, s. epidermidis, s. saprophyticus |
|
Definition
S aureus is Beta hemolytic
epidermidis and saprophyticus are not hemolytics |
|
|
Term
|
Definition
S. aureus
not
s staprophyticus and
s epidermidis |
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|
Term
| Which strain of staph has coagulase and DNAse |
|
Definition
|
|
Term
what are the differences between
streptococcus pneumoniae and step mutans
|
|
Definition
| rmutans is resistant to optochin |
|
|
Term
| what is the difference between group a and b Beta strep |
|
Definition
| group a doesn't produce CAMP, resistant to bacitracin |
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|
Term
Enterobacteriaceae
gram?
shape?
catalase? |
|
Definition
gram negative
rod
catalase positive |
|
|
Term
enterobacteriacea
staphylococcus
streptococcus
gram?
catalase?
shape?
|
|
Definition
enterobacteriaceae: single gram negative bacillus, catalase positive
staph: gram positive coccus clusters, catalase positive
strep; gram positive coccus chains, catalase negative |
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