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Biology 111 Lab Practical #1
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56
Biology
Undergraduate 1
02/10/2013

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Term
two main periods of eukaryotic cell life
Definition
interphase and mitosis
Term
G1
Definition
cell grows; cell is carrying out biosynthetic activities that are characteristic for that cell
Term
S
Definition
DNA is replicated
Term
G2
Definition
centrosomes replicate and chromosomes begin condensing
Term
Prophase
Definition
centrosomes move to opposite sides of nucleus, chromosomes finish condensing; mitotic spindle begins to form
Term
Prometaphase
Definition
nuclear membrane breaks down; spindle fibers begin connecting to chromosomes at kinetochore protein
Term
Metaphase
Definition
when the spindles have adjusted their length so that all of the chromosomes are on the metaphase plate
Term
Anaphase
Definition
cohesin is dissolved and chromatids separate
Term
Telophase
Definition
chromatids are at their respective poles; cytokinesis is occurring; nuclear membrane forms; chromosomes decondense
Term

2C DNA

(mitosis)

Definition
G1
Term

transition btwn. 2C-4C DNA

(mitosis and meiosis)

Definition
S Phase
Term

4C DNA

(mitosis)

Definition
G2, Prophase, Prometaphase, Anaphase, Telophase
Term
mean
Definition
ordinary average of observations
Term
standard deviation
Definition
measures how spread out the observations are, starting from mean as center
Term
variance
Definition
an average of the squared deviations of the individual oberservations from their mean
Term

null hypothesis

(H0)

Definition

says that there is no difference btwn. the means of the 2 populations that our samples represent; the 2 samples came from the same source

*chance alone causes the difference in data collected

Term

Alternative Hypothesis

(H1)

Definition

says that there is a difference in the mean weights of the 2 populations of beans; difference in weights for samples is not due to variation

*differences in data collected are due to a real difference or factor

Term
t ratio in a parametric test
Definition

t ratio is a comparison of the size of the difference btwn. the means of the 2 samples relative to how much uncertainty there is in the vaule of these means

*larger t ratio=more likely to reject null hypothesis

Term
how to calculate t ratio
Definition

x1-x2

su

 

 

 

 

Term
su in t ratio
Definition

standard error of difference

 

(s12+s22)1/2

(n1     n2 )   

s1=standard deviation of one sample

s2=standard deviation of other sample

n1=number in one sample

n1=number in other sample

Term
t value of <0.05:
Definition

strong evidence against null

*reject null

Term
t value >0.05:
Definition
accept null hypothesis
Term
degrees of freedom for t test:
Definition

# observations -2

 

n1+n2-2

 

n1=number beans

n2=number beans

Term
nominal measurements
Definition

result whe you simply categorize a thing or property

*t test is of no use for nominal measurements, only for ratio measurements

Term
Chi-Test
Definition
non-parametric test; used for nominal measurements
Term

Chi-Square

(X2)

Definition

(O-E)2

E

 

O=observed

E=expected

 

Add all values of the above to get X2

Term
large values of X2:
Definition

good evidence against null; large difference btwn O and E

*reject null

Term
Small values of X2:
Definition

Small difference btwn. O and E

*Accept null hypothesis

Term
Degrees of Freedom for Chi Test:
Definition
number of categories - 1
Term
transmission genetics
Definition
rules by which offspring inherit characteristics from their parents
Term
meiosis
Definition
process that produces cells which contain one chromosome from each pair of chromosomes that started the process
Term
gametes
Definition
egg and sperm
Term
spores
Definition
cells that grow into gametophytes of familiar plants
Term
fertilization
Definition
chromosomes of one gamete are added to the chromosomes of the other, restoring diploid number
Term
physical traits result from:
Definition
genes and the environment
Term
locus
Definition
location of gene on a chromosome
Term
homologous chromosomes
Definition
same size and shape; same loci in same order
Term
allele
Definition
two genes that occupy corresponding loci on homologous chromosomes
Term
street analogy
Definition

chromosomes are the street

the lots along the street is a locus

the lots contain houses, and the houses are alleles

Term

2C DNA

(meiosis)

Definition
G1, Interkinesis, PII, MII AII, TII
Term

1C DNA

(Meiosis)

Definition
Gamete Spores
Term

4C DNA

(meiosis)

Definition
G2, PI, MI, AI, TI
Term
standard phenotype for Drosophila
Definition

wild type

*red eye color pr+ (dominant)

* smooth wings W+  (recessive)

 

Term
mutant phenotype for Drosophila
Definition

purple eye color "pr" (recessive)

wrinkled wings "W" (dominant)

Term
difference btwn. male and female Drosophila
Definition

females are larger than males and have a fully striped abdomen

males have a solid black tipped body and have sex combs on their forelegs

Term
eye color genotypes of Drosophila
Definition

se+: red eye color

se: sepia eye color

Term
body color phenotype of Drosophila
Definition

brown: dominant

black: recessive

Term
wing genotype for Drosophila
Definition

vg+: wild type large wings

vg: mutant type vestigial wings

Term
pigment color of Brassica rapa
Definition

ANL: allows purple color to appear (dominant)

anl: blocks pigment (recessive)

Term
Height of Brassica rapa
Definition

D: tall plant (dominant)

d: short plant (recessive)

Term
Leaf development of Brassica rapa
Definition

Ragged egdes: Rg (dominant)

smooth edges: rg (recessive)

Term
recombination
Definition
produces gene combinations in the gametes of an organism that are different from the gene combinations that were in the gametes that fused to make that organism
Term
Percent Recombination
Definition

# recombinant / total # indiviauals X 100

PR=Map Distance cM

Term
seed color os Brassica rapa
Definition

normal seed color: C (dominant)

no seed color: c (recessive)

Term
Endosperm genotype of Brassica rapa
Definition

normal endosperm: Wx (dominant)

waxy endosperm: wx (recessive)

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