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BIOL LAB FINAL
final exam
47
Biology
Undergraduate 1
11/26/2012

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Term
Blank
Definition
contains everything but what is being measured
Term
Beer's law
Definition
as the concentration of a substance increases, the amount of light absorbed by the solution increases
Term
activation energy
Definition
the energy required to make the reaction happen
Term
enzyme
Definition
catalyst that speeds up a reaction by reducing the activation energy
Term
spectrophotometer
Definition
used to show the relationship between light and the concentration of substances
Term
standard deviation
Definition
how far something tends to stray from the average
Term
mean
Definition
represents the central value of data
Term
volumetric pipets
Definition
designed to deliver a single volume
Term
green pipet
Definition
10 mL
Term
blue pipet
Definition
1 mL
Term
diffraction grating
Definition
light dispersion device
Term
what makes up biological membranes
Definition
phospholipid bilayer
Term
diffusion
Definition
spontaneous NET movement of particles from an area of high concentration to an area of low concentration
Term
two factors that affect the movement of materials across a cell membrane
Definition
1) permeability
2) concentration
Term
higher permeability means less ____
Definition
time
Term
two phases of photosynthesis
Definition
1) light reactions
2) Calvin cycle
Term
mesophyll
Definition
layer of leaf cross section that serves as a place of gas exchange
Term
types of fermentation
Definition
lactate & alcohol
Term
pigments in descending order of quickness
Definition
xanthophylls > carotene > chlorophyll a > chlorophyll b
Term
mechanical pipets
Definition
used for small, high precision volume transfers
Term
steps to working spectrophotometer
Definition
1) set to 0% transmittance
2) place the blank
3) set to 100% transmittance
4) switch mode to absorbance
5) replace blank with sample
Term
steps of the scientific method
Definition
1) models - explanation or theories
2) null hypothesis
3) alternative hypothesis
4) experiment
5) observations
6) interpretation
Term
two isomers of catechol
Definition
1) resorcinol
2) hydroquinone
Term
catecholase
Definition
enzyme that catalyzes the reaction of benzoquinone
Term
activation site
Definition
substrate binding site of an enzyme
Term
osmosis
Definition
NET movement of water from an area of low concentration to an area of high concentration
Term
hypotonic
Definition
has less solute
Term
hypertonic
Definition
has more solute
Term
isotonic
Definition
has the same amount of solute
Term
hemolysis
Definition
rapid swelling and rupturing of a RBC
Term
crenation
Definition
shrinking and shriveling of RBC
Term
what does IRGA stand for?
Definition
infrared gas analyzer
Term
how is catechol transformed into benzoquinone?
Definition
oxidation reaction
Term
inputs of photosynthesis
Definition
light, CO2, H2O
Term
outputs of photosynthesis
Definition
O2 & a sugar molecule
Term
net rate of photosynthesis
Definition
oxygen produced minus oxygen consumed by aerobic respiration
Term
2 layers of mesophyll
Definition
1) palisade layer
2) spongy layer
Term
stoma
Definition
where carbon dioxide diffuses into the spongy layer of the mesophyll
Term
where is the stoma located
Definition
lower epidermis
Term
pigments
Definition
substances that absorb lights in light reaction of photosynthesis
Term
the color of an object is composed of wavelengths that are______
Definition
absorbed & transmitted
Term
why can you see red fluorescence with the green spinach extract when shining a flashlight?
Definition
when something becomes light saturated, red fluorescence is given off
Term
two phases of chromatographic systems
Definition
1) mobile
2) stationary
Term
in the fermentation reaction, pyruvates are converted into ______
Definition
ethanol & lactid acid
Term
is fermentation aerobic or aneorobic
Definition
aneorobic
Term
DNA has a ____ charge
Definition
negative
Term
phaeophytins
Definition
the grey bands between xanthophylls and carotene; breakdown product of chlorophyll a and b
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