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BIOL 252 Lab
Blood Lab 1
34
Biology
Undergraduate 2
02/24/2008

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Cards

Term
Hematocrit
Definition
Percent volume of whole blood that is occupied by red blood cells
Term

Centrifuge blood in "hematocrit" capillary tubes

 

 

Definition
How to determine hematocrit
Term
Anemia
Definition
Hematocrit falling to 15% is known as
Term
Polycythemia
Definition
Condition where hematocrit rises to as high as 70%
Term

Avg: 46%

Range: 43-49% 

Definition

Normal Hct value for males:

Avg

Range 

Term

Avg: 41%

Rang: 36-45%

Definition

Normal Hct value for females:

Avg

Range

Term
[ht of red cells (mm)/ht of red cells+plasma(mm)] x 100
Definition
Formula for Hct % is:
Term

1. Oxygen transportation

2. Acid-base buffer

3. Destroying foreign organisms

4. Distribution of body heat

5. Coagulation 

Definition
5 Functions of Blood
Term
15 g
Definition
___ g of hemoglobin are in 100 ml of blood
Term

Cyanmethemoglobin test

potassium cyanide (KCN) 

 

Definition
______ test involves reaction of hemoglobin with a reagent containing ______
Term
Standard curve
Definition
The _________ is used to compare amts of light absorbed by a specimen compared to known concentration of hemoglobin
Term
(Total g Hb) x (1.34 ml O2/g Hb)
Definition
Total O2 carrying capacity=
Term

blood volume (ml) x hemoglobin concentration

                            (g Hb/ml blood)

Definition
Total blood Hb (g) =
Term
70 ml
Definition
A rat has approximately ____ ml of blood per kg body weight
Term
79 ml blood per kg body weight +/- 10%
Definition
Blood volume for human males
Term
65 ml blood per kg body weight +/- 10%
Definition
Blood volume for human females
Term
2.2 lbs = ____ kg
Definition
1 kg = ____ lbs
Term
7500/cubic mm on avg
Definition
Total leukocyte count per cubic mm
Term
Protozoan infections, malnutrition, aplastic anemia
Definition
Diseases associated with neutrophilic leukopenia
Term
Strenuous exercise, rheumatic fever, severe burns
Definition
Afflications assoc w/ Neutrophilic leukocytosis
Term
Mumps, German measles, whooping cough
Definition
Afflications assoc w/ lymphocytosis
Term
Scarlet fever, parasitic infections, allergic reactions
Definition
Afflications assoc w/ Eosinophilia
Term
Chronic diseases, such as tuberculosis & leukemia
Definition
Afflications assoc w/ Monocytosis
Term
Administration of glucocorticoid drugs
Definition
Afflictions associated w/ Lymphocytopenia
Term

aka Polymorphonuclear Leukocytes

1. Neutrophils

2. Eosinophils

3. Basophils 

Definition
3 types of Granulocytes (aka __________)
Term
Granulocytes
Definition
Polymorphonuclear leukocytes aka
Term

aka Mononuclear Leukocytes

1. Small lymphocytes

2. Large lymphocytes

3. Monocytes 

Definition

3 types of Agranulocytes (aka ____) 

Term

65%

10-12 microns

Multi-lobed (3 or more) nucleus

Small pink cytoplasmic granules

Purple nucleus 

Definition

Neutrophils make up _% of total WBC

____ microns diam.

______ nucleus

_____ cytoplasmic granules

_____ nucleus 

Term

2-4%

14 microns

Bilobed nucleus

Coarse red-orange granules

Blue-purple nucleus 

Definition

Eosinophils make up _% of total WBC

____ microns diam.

______ nucleus

_____ cytoplasmic granules

_____ nucleus

Term

0.5%

14 microns

Bilobed nucleus

Large, deep-blue or reddish-purple granules

Black nucles obscured by granules 

Definition

Basophils make up _% of total WBC

____ microns diam.

______ nucleus

_____ cytoplasmic granules

_____ nucleus

Term

20-25%

7 microns

Very large, spherical nucleus w/ thin cytoplasm

Light blue cytoplasm

Deep blue or purple nucleus 

Definition

Small lymphocytes make up _% of total WBC

____ microns diam.

______ nucleus

_____ cytoplasm (nongranular)

_____ nucleus

Term

3%

10 microns

Large oval, indented nucleus

Light blue cytoplasm

Dark purple nucleus 

Definition

Large lymphocytes make up _% of total WBC

____ microns diam.

______ nucleus

_____ cytoplasm (nongranular)

_____ nucleus

Term

3-7%

15 microns

Large, deeply indented nucleus (horseshoe-shaped)

Light blue-gray cytoplasm

Blue or purple nucleus 

Definition

Monocytes make up _% of total WBC

____ microns diam.

______ nucleus

_____ cytoplasm (nongranular)

_____ nucleus

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