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Biochemical tests
Biochemical testing for the lab
7
Microbiology
Undergraduate 2
05/04/2011

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Term
what is the medium and substrate in reduction of nitrates biochemical test?
Definition
the medium is nitrate broth and the substrate is KNO3, recall that after pottasium nitrate gets reduced it releases KNO2 potassium nitrite and ATP...
Term
What are the ingredients of Litmus Milk ?
Definition
Whole millk and the pH indicator Litmus, which is pink at 6.8pH or below, purple from 6.9 to 8.0 pH, and blue from 8.0 and above pH.
Term
What are the various results that may be observed from an organism's inoculation in Litmus milk ?
Definition
Fermentation of litmus, Production of acid curd, production of gas, alkalinization, Peptonization, production of rennet curd, and reduction of litmus.
Term
where does leucolitmus come from and what does it do ?
Definition
In the biochemical test Reduction of Litmus, some microorganisms reduce the litmus forming leucolitmus. Litmus acts as the final electron acceptor when oxygen is unavailable, as a result of this reaction, the litmus looses its litmus color which is purple or a purple hue...
Term
What is alkalinization and how does it change the pH of litmus milk?
Definition
Alkalinization is the incomplete hydrolysis of the milk protein casein into shorter polypeptide chains. This process produces alkaline end products which raise the pH of litmus changing its color to blue.
Term
What is the substrate in the fermentation reaction of litmus milk?
Definition
Lactose:  When lactose gets fermented, it produces various organic acids as well as ATP, the formation of this organic acids changes the pH of the pH indicator the Pink.
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