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Biochemical Tests
N/A
8
Microbiology
Undergraduate 4
11/30/2010

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Cards

Term
Carbohydrate Test
Definition
- bromocresol purple
- fementation of simple carbs
Term
Catalase Test
Definition
- 2 H2O2 --> H2O + O2
- catalase is only produced by viable cells
Term
Citrate Usage
Definition
- tests ability to use citrate as sole carbon source
- bromothymol blue
- sodium carbonate produced --> PH lowers
Term
Coagulase Test
Definition
- used to differentiate S. aureus strains (+) from other
- coagulase and/or clumping factor
-
Term
Hugh-Leifson O/F
Definition
- bromothymol blue (yellow if acid produced)
- blue if peptone deaminated
Term
Indole
Definition
- by product of metabolic break down of tryptophan
- Kovac's reagent with indole --> red
-
Term
Methyl Red
Definition
- differentiate gram-negative enteric bacteria
- butylene glycol pathway of glucose metabolism
- identify bacteria that use the mixed-acid pathway
- metabolize pyruvic acid
Term
Vogues-Proskauer
Definition
- butylene glycol pathway of glucose metabolism
- detects acetoin
- positive --> red
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