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biochem
biochem 1
59
Biology
Graduate
10/25/2009

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Term
ribose
Definition
A pentose sugar, C5H10O5, occurring as a component of riboflavin, nucleotides, and nucleic acids.
Term
chromatin
Definition
A complex of nucleic acids and proteins, primarily histones, in the cell nucleus that stains readily with basic dyes and condenses to form chromosomes during cell division.
Term
hybridization
Definition
To form base pairs between complementary regions of (two strands of DNA that were not originally paired)
Term
codon
Definition
A sequence of three adjacent nucleotides constituting the genetic code that determines the insertion of a specific amino acid in a polypeptide chain during protein synthesis or the signal to stop protein synthesis
Term
nucleotide
Definition
Any of various compounds consisting of a nucleoside combined with a phosphate group and forming the basic constituent of DNA and RNA
Term
deoxyribose
Definition
a pentose sugar obtained by the hydrolysis of DNA. Formula: C5H10O4
Term
complementary
Definition
Of or relating to the specific pairing of the purines and pyrimidines between strands of a DNA or an RNA molecule
Term
ribosome
Definition
A minute round particle composed of RNA and protein that is found in the cytoplasm of living cells and serves as the site of assembly for polypeptides encoded by messenger RNA
Term
nucleosome
Definition
Any of the repeating subunits of chromatin, consisting of a DNA chain coiled around a core of histones
Term
nucleoside
Definition
Any of various compounds consisting of a nucleoside combined with a phosphate group and forming the basic constituent of DNA and RNA
Term
nucleoside (good)
Definition
Any of various compounds consisting of a sugar, usually ribose or deoxyribose, and a purine or pyrimidine base, especially a compound obtained by hydrolysis of a nucleic acid, such as adenosine or guanine
Term
poly (A) tail
Definition
sequence of adenine nucleotides at the 3' end of mRNA
Term
anticodon
Definition
A sequence of three adjacent nucleotides in transfer RNA that binds to a corresponding codon in messenger RNA and designates a specific amino acid during protein synthesis
Term
histone
Definition
Any of several small, basic proteins most commonly found in association with the DNA in the chromatin of eukaryotes
Term
antiparallel
Definition
parallel but oppositely directed
Term
transcription
Definition
The process by which messenger RNA is synthesized from a DNA template resulting in the transfer of genetic information from the DNA molecule to the messenger RNA
Term
template strand
Definition
is the strand of DNA that the RNA polymerase uses as a guide to build complementary mRNA. This is the reverse complement of the coding strand
Term
coding strand
Definition
is the DNA strand which has the same base sequence as the RNA transcript produced (although with thymine replaced by uracil)
Term
promotor sequence
Definition
a promoter is a regulatory region of DNA located upstream (towards the 5' region) of a gene, providing a control point for regulated gene transcription
Term
primary transcript
Definition
is an RNA molecule that has not yet undergone any modification after its synthesis. After splicing the transcript forms messenger RNAs, ribosomal RNAs, transfer RNAs, or ribozymes
Term
upstream
Definition
Relative to the position on the strand, upstream is the region towards the 5' end of the strand.
Term
downstream
Definition
Relative to the position on the strand, downstream is the region towards the 3' end of the strand.
Term
free radical
Definition
A molecule with an unpaired electron. Because they have a free electron, such molecules are highly reactive
Term
reactive oxygen species
Definition
Species such as superoxide, hydrogen peroxide, and hydroxyl radical. At low levels, these species may function in cell signalling processes. At higher levels, these species may damage cellular macromolecules (such as DNA and RNA) and participate in apoptosis
Term
acid
Definition
hydrogen ion donors
Term
base
Definition
hydrogen ion acceptors
Term
weak acid
Definition
small % of total molecules dissociate
Term
strong acid
Definition
dissociate and release their hydrogen ions (protons)
Term
buffer
Definition
mixture of an undissociated acid and its conjugate base (the form of an acid that has lost its proton); resist changes in pH
Term
2 things determine effictivness of buffer
Definition
its pKa relative to pH and and its concentration of solution
Term
sodium is pumped in or out of cell?
Definition
out
Term
potassium is pumped out or into cell?
Definition
into the cell
Term
major cation/anion ECF
Definition
sodium and chloride
Term
major cation/anion ICF
Definition
potassium, inorganic phosphate
Term
pH
Definition
-log[H+]
Term
Ka
Definition
tendency of an acid to dissociate and donate a H ion to solution
Term
domain
Definition
a structurally compact portion of a protein molecule
Term
motif
Definition
relatively small arrangements of 2nd structure that are recognized in many different proteins
Term
fold
Definition
relatively large patterns of 3-d structure that have been recognized in many proteins; have characteristic activity
Term
protomer
Definition
unit structure composed of non identical subunits (quaternary stucture)
Term
oligomer
Definition
multisubunit protein composed of identical subunits
Term
myoglobin
Definition
8 alpha helix; higher affinity than hemoglobin; hyperbolic curve
Term
prosthetic group
Definition
nonprotein ligands bound tightly
Term
holoprotein
Definition
protein bound to prosthetic group
Term
aprotein
Definition
protein not bound to prosthetic group
Term
enzyme
Definition
Any of numerous proteins or conjugated proteins produced by living organisms and functioning as biochemical catalysts
Term
active site
Definition
The part of an enzyme at which catalysis of the substrate occurs
Term
coenzyme
Definition
A nonproteinaceous organic substance that usually contains a vitamin or mineral and combines with a specific protein, the apoenzyme, to form an active enzyme system
Term
cofactor
Definition
is a substance that needs to be present in addition to an enzyme for a certain reaction to take place.
Term
endocrine hormone
Definition
enters blood and exerts its actions on specific target cells
Term
paracrine hormone
Definition
performed on nearby cells (acetylcholine)
Term
autocrine hormone
Definition
acts on the cell that secreted it
Term
topoisomerases
Definition
An enzyme which introduces or removes overwinding or underwinding of the DNA circular duplex by causing a nick, rotating the strands, and then ligating them
Term
solonoid
Definition
a complex made up of lots nuclesomes (histone complexes)
Term
helicase
Definition
motor proteins that move directionally along a nucleic acid phosphodiester backbone, separating two annealed nucleic acid strands (i.e. DNA, RNA, or RNA-DNA hybrid) using energy derived from nucleotide hydrolysis
Term
processitivity
Definition
how long DNA polymerase will work before it falls off
Term
fidelity
Definition
making mistakes during replication
Term
AP site
Definition
apurinic or apyrimidine site (lack base in DNA chain)
Term
superantigens
Definition
a substance (as an enterotoxin) that acts as an antigen capable of stimulating much larger numbers of T cells than an ordinary antigen
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